Identification and Construction of RCAS RNA Interference Vector for Chicken Vezf1

doi:10.13560/j.cnki.biotech.bull.1985.2015.08.015

Abstract

Abstract:

In order to study the role of Vezf1 during the earlier development of chicken embryo, RNA interference vector for the chicken Vezf1 was constructed based on RCAS viral vector system, and the gene silencing of chicken Vezf1 was carried out in both embryonic cells and chicken embryos, and the mRNA expression was measured by Real-time PCR and in situ hybridization. Results showed that this interference vector from RCAS virus could keep Vezf1 expression silencing successfully in chicken embryo fibroblasts and chick embryos. This study provides a fundamental data for studying the Vezf1’s role during early embryonic development using the chicken model.

Key words: RCAS vector, RNAi, Vezf1, chicken embryo

Zhang Chao, Ge Jun, Yuan Mingjing, Ye Jun, Yuan Li. Identification and Construction of RCAS RNA Interference Vector for Chicken Vezf1[J]. Biotechnology Bulletin, 2015, 31(8): 102-107.

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