Isolation and Characterization of the Chlorpyrifos-degrading Trichoderma Strains from the Vegetable Soil in Greenhouse

doi:10.13560/j.cnki.biotech.bull.1985.2016.06.030

Abstract

Abstract: To explore and protect the Trichoderma resources that can degrade chlorpyrifos，six different types of Trichoderma strains with high activity of degrading chlorpyrifos were screened from the long-term organophosphorus pesticide contaminated soil. Analyses of morphological characteristics combined with internal transcribed spacer(ITS)rDNA sequences were used to identify them as T. harzianum, T. viride, T. koningii, T. koningiopsis, T. longibrachiatum，and T. brevicompactum，respectively. T. longibrachiatum TC5 with the highest degradation activity was selected to investigate the characterization of degrading chlorpyrifos under the culture condition including extra carbon source，pesticide concentration，inoculum density and pH. Under neutral or alkaline conditions，the strains TC5 had solid degradation activity to chlorpyrifos. Either adding carbon or increasing the inoculum concentration promoted the degradation rate. Degradation activity gradually increased with the increasing of chlorpyrifos concentration in the range of 50-300 mg/L. In the pot experiment，Trichoderma TC5 remained the high degradation activity to chlorpyrifos，but the degradation activity in the natural soil was significantly lower than in sterilized soil. The Trichoderma strains have great potential application in remedying the pesticide-contaminated soil.

Key words: Trichoderma, chlorpyrifos, biodegradation

ZHANG Guang-zhi, ZHANG Xin-jian, LI Hong-mei, GUO Kai, YANG He-tong. Isolation and Characterization of the Chlorpyrifos-degrading Trichoderma Strains from the Vegetable Soil in Greenhouse[J]. Biotechnology Bulletin, 2016, 32(6): 205-210.

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