Application of Switch for Synthetic Biology in Metabolic Engineering

doi:10.13560/j.cnki.biotech.bull.1985.2017.01.006

Abstract

Abstract: Translational switches for synthetic biology are mainly used to regulate gene expression in the field of metabolic engineering research. Traditional metabolic engineering regulates the expression of specific genes by using knockout and overexpression. However,the knockout of genes usually leads to the decrease in growth,therefore,we need to close and activate the expression of specific gene at proper time,and translational switche for synthetic biology is the key tool to solve such issue. At present,light-controlled switch,temperature-induced switch,toggle switch,and riboswitch are commonly used in metabolic engineering,of which the toggle switch and riboswitch present a great advantage in the dynamic regulation of gene expression. In this paper,several kinds of translational switches for synthetic biology and their applications in metabolic engineering are reviewed.

Key words: synthetic biology, gene expression regulation, metabolic engineering, switch for synthesis biology, dynamic regulation

PANG Qing-xiao, LIANG Quan-feng, QI Qing-sheng. Application of Switch for Synthetic Biology in Metabolic Engineering[J]. Biotechnology Bulletin, 2017, 33(1): 58-64.

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