Research Progresses on the Role of Transport Proteins NupC and NupG During the Nucleoside Secretion Processing in Microbial Cells

doi:10.13560/j.cnki.biotech.bull.1985.2017.02.004

Abstract

Abstract: The nucleosides are used extensively,and microbial fermentation is one of main methods of producing it. The secretion of nucleoside in microbial cells plays a critical role for high-yield nucleoside. There are many transport proteins as the role of transportation in the cell membrane,which are involved in the secretion of metabolites. Here,this article mainly elaborates the role of nucleoside transport proteins such as NupG and NupC during the nucleoside secretion process in microbial cells. Additionally,the effects of modification for transport protein on nucleoside biosynthesis were introduced,aiming at providing evidence for the breeding strategy of engineering high-yield strains.

Key words: microbes, nucleoside transporter, NupC, NupG

WANG Meng-jiao, FANG Hai-tian, LIU Hui-yan, HE Xiao-guang, WU Qing, ZHAO Bei-bei. Research Progresses on the Role of Transport Proteins NupC and NupG During the Nucleoside Secretion Processing in Microbial Cells[J]. Biotechnology Bulletin, 2017, 33(2): 24-29.

References

[1] 刘永康, 陈国民. 细胞膜对大分子物质及颗粒物质的跨膜转运[J]. 现代医药卫生, 2006（5）：674-675.
[2] 马蓉, 张立军, 丁锐, 等. 大肠杆菌氨基酸转运蛋白的研究进展[J]. 科技通报, 2012, 3：49-56, 99.
[3] 王晶敏. 核苷转运蛋白在细胞水平跨膜转运作用的研究进展[J]. 国外医学·药学分册, 2003, 1：17-22.
[4] 李剑, 李光永, 王道文. 核苷转运蛋白的研究进展[J]. 科学通报, 2002, 7：481-484.
[5] Martinussen J, Willemoës M, Kilstrup M. Nucleotide metabolism [M]. Second Edition. Comprehensive Biotechnology, 2011：91-107.
[6] Charles L. Turnbough Jr, Robert L, et al. Regulation of pyrimidine biosynthetic gene expression in bacteria：repress ion without repressors[J]. Microbiology and Molecular Biology Reviews, 2008, 72（2）：266-300.
[7] Paulsen IT, Sliwinski MK, Saier MH. Microbial genome analyses：Global comparisons of transport capabilities based on phylogenies, bioenergetics and substrate specificities[J]. J Mol Biol, 1998, 277：573-592.
[8] http//bioweb. pasteur. fr/seqanal/interfaces/toppred. html
[9] Madej T, Lanczycki CJ, Zhang D, et al. MMDB and VAST+：tracking structural similarities between macromolecular complexes[J]. Nucleic Acids Res, 2014, 42（Database issue）：D297-303.
[10] Villas-Boas S, Han TL, Liu T, et al. What is the relationship between intracellular and extracellular metabolites? The theory of “metabolic overflow” put into test[J]. New Biotechnology, 2014, 31：S28-S29.
[11] 张星元. 发酵原理[M]. 北京：科学出版社, 2011.
[12] Baranyi J, Metris A, George SM. Bacterial economics：Adaptation to stress conditions via stage-wise changes in the response mechanism[J]. Food Microbiology, 2015, 45：162-166.
[13] Chokkathukalam A, Kim DH, Barrett MP, et al. Stable isotope-labeling studies in metabolomics：new insights into structure and dynamics of metabolic networks[J]. Bioanalysis, 2014, 6（4）：511-524.
[14] 张星元, 李秀敏, 杨毅, 等. 谷氨酸棒杆菌的氨基酸输送系统的存在、功能及其在氨基酸生产上的重要性[J]. 无锡轻工大学学报, 2004, 23（3）：105-110.
[15] 姚辉, 张建华, 毛忠贵. 谷氨酸棒状杆菌的谷氨酸分泌模式初探[J]. 食品与发酵工业, 2013, 39（5）：54-58.
[16] Eggeling L, Krumbach K, Shahm H. L-Glutamate efflux with Corynebacterium glutamicum：why is penicillin treatment or Tween addition doing the same[J]. J Mol Microbiol Biotechnol, 2001, 3（1）：67-68.
[17] Puech V, Chami M, Lemassu A, et al. Structure of the cell envelope of corynebacteria：importance of the non-covalently bound lipids in the formation of the cell wall permeability barrier and fracture plane[J]. Microbioloy, 2001, 147：1365-1382.
[18] Ramadan A, Naydenova Z, Stevanovic K, et al. The adenosine transport ENT1 in cardiomyocytes is sensitive to inhibition by ethanol in akinase-dependent manner：implications for ethanol-dependent cardioprotection and nucleoside analog drug cytotoxicity[J]. Purinergic Signalling, 2014, 10（2）：305-312.
[19] Zhu H, Yang SM, Yuan ZM, et al. Metabolic and genetic factors affecting the productivity of pyrimidine nucleoside in Bacillus subtilis[J]. Microbial Cell Factories, 2015, 14：54.
[20] Song BH, Jan N. Chromosomal location, cloning and nucleotide sequence of the Bacillus subtilis cdd gene encoding cytidine/deoxycytidine deaminase[J]. Mol Gen Genet, 1989;216：462-468.
[21] Saxild HH, Andersen LN, Hammer K. Dra-nupC-pdp operon of Bacillus subtilis：nucleotide sequence, induction by deoxyribonucleosides, and transcriptional regulation by the deoR-encoded DeoR repressor protein[J]. J Bacteriol, 1996, 178：424-434.
[22] Henderson PJ, Herbert RB. The nucleoside transport proteins, NupC and NupG, from Escherichia coli：specific structural motifs necessary for the binding of ligands[J]. Org Biomol Chem, 2005, 3：462-470.
[23] Karatza P, Frillingos S. Cloning and functional characterization of two bacterial members of the NAT/NCS2 family in Escherichia coli[J]. Mol Membr Biol, 2005, 22（3）：251-261.
[24] Munch-Petersen A, Jensen N, et al. Cloning and expression of genes encoding the nupG nucleoside transport system in Escherichia coli[J]. The Cell Membran, 1984, 411（2）：85-99.
[25] Zhu H, Yang SM, Yuana ZM, et al. Metabolic and genetic factors affecting the productivity of pyrimidine nucleoside in Bacillus subtilis[J]. Microbial Cell Factories, 2015, 14（54）：2-12 .
[26] Jardetzky O. Simple allosteric model for membrane pumps[J]. Nature, 1966, 211：969-970.
[27] Mitchell P. A general theory of membrane transport from studies of bacteria[J]. Nature, 1957, 180（4577）：134-136.
[28] Smirnova I, Kasho V, Choe JY, et al. Sugar binding induces an outward facing conformation of LacY[J]. Proc Natl Acad Sci USA, 2007, 104：16504-16509.
[29] Monné M, Chan KW, Slotboom DJ, et al . Functional expression of eukaryotic membrane proteins in Lactococcus lactis[J]. Protein Sci, 2005, 14（12）：3048-3056.
[30] Abramson J, Smirnova I, Kasho V, et al . Structure and mechanism of the lactose permease of Escherichia coli[J]. Science, 2003, 301（5633）：610-615.
[31] Huang Y, Lemieux MJ, Song JM. Structure and mechanism of theglycerol-3-phosphate transporter from Escherichia coli[J]. Science, 2003, 301（5633）：616-620.
[32] 赵伟睿, 胡升, 黄俊, 等. 微生物细胞通透性改善方法与策略[J]. 中国生物工程杂志, 2014, 34（3）：125-131.
[33] Yamada S, Awano N, Inubushi K, et al. Effect of drug transporter genes on cysteine export and overproduction in Escherichia coli[J]. Applied and Environmental Microbiology, 2006, 72（7）：4735-4742.
[34] Kuhn JG. Fluorouracil and the new oral fluorinated pyrimidines[J]. Ann Pharmacother, 2001, 35（2）：217-227.
[35] Mohanty AK, Wiener MC. Membrane protein expression andproduction：effects of polyhistidine tag length and position[J]. Protein Expr Purif, 2004, 33（2）：311-325.
[36] Sheremet AS, Gronskiy SV, Akhmadyshin RA, et al. Enhancement of extrancellular purine nucleoside accumulation by Bacillus strains through genetic modifications of genes involved in nucleoside export[J]. Jlnd Microbiol Biotechnol, 2011, 38（1）：65-70.