Screen of Poly-γ-gamma Glutamic Acid Producing Bacteria and Their Glutamic Acid Fermentation Broth’s Function on the Drought Resistance of Maize

doi:10.13560/j.cnki.biotech.bull.1985.2017-0699

Abstract

Abstract: As a novel biological water absorbent，poly-γ-gamma glutamic acid（γ-PGA）has a significant application value on water-saving agricultural production. Two γ-PGA-producing strains，Y2 and P1，were isolated and screened from natto and fermented soya bean samples. By analyzing their morphological and physic-chemical characters，and 16S rRNA sequence，they were identified as Bacillus subtilis. Then，the effects of γ-PGA fermentation broth on the soil water-holding capacity and the drought resistance of maize seedling under water stress were also studied. The results showed that the fermentation broth of Y2 and P1 significantly decreased the soil water evaporation. Compared with the control treatment，adding fermentation broth from γ-PGA-producing strain promoted the soil water content by 4%. At the same time，the addition of γ-PGA fermentation broth enhanced the drought resistance of maize seedlings，and significantly increased aboveground and underground biomass of maize seedlings by 40.70% and 19.59%，respectively. The result of biologECO showed that the γ-PGA fermentation broth from Y2 and P1 had insignificant effect on microbial diversity and evenness. Thus，the γ-PGA fermentation broth is an environmental friendly biological water absorbent，which can be used directly to enhance soil water retention and drought resistance of vegetation in field.

Key words: poly-γ-glutamic acid, drought resistance, drought stress, soil microbes

JIA Yan-ping, YIN Ai-ming, SUN Yan-mei, CHENG Shou-tao, WANG Xu-ming. Screen of Poly-γ-gamma Glutamic Acid Producing Bacteria and Their Glutamic Acid Fermentation Broth’s Function on the Drought Resistance of Maize[J]. Biotechnology Bulletin, 2017, 33(10): 135-142.

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