Research Progress on Male Sterility of Pepper

doi:10.13560/j.cnki.biotech.bull.1985.2017-0207

Abstract

Abstract: As a common and special physiological phenomenon in the plant kingdom，male sterility has high research values in basic application and practical production. In this paper，we review the pepper genic male sterility，cytoplasmic male sterility and the different abortion ways of pollen，as well as different periods of microspore abortion，and then compare some of the pollen development physiological and biochemical phenomena，mainly related to material metabolism and energy metabolism. Further，we introduce the relationship between male sterility and mitochondria from the aspects of mitochondrial metabolism，the structure and number of mitochondria，and the molecular basis of mitochondria，as well as the relevant studies on the markers and proteomics of male sterile genes using molecular technologies，so as to provide references for pepper breeding researches.

Key words: pepper, male sterility, gene marker, proteomics, mitochondrial metabolism

SHAO Gui-fang, ZHANG Fan, WANG Jiao, ZHAO Kai, MO Yun-rong, DENG Ming-hua. Research Progress on Male Sterility of Pepper[J]. Biotechnology Bulletin, 2017, 33(8): 7-13.

References

[1] 吴峰, 刘玉梅, 孙德岭, 等. 辣椒胞质雄性不育系与保持系生化特性研究[J] . 天津农业科学, 2008, 14（2）：50-52.
[2] 石雅丽, 张锐, 任茂智, 等. 棉花雄性不育的研究进展[J] . 生物技术进展, 2013, （5）：328-335.
[3] 杨世周, 赵雪云. 辣椒8021A雄性不育系的选育及三系配套[J] . 中国蔬菜, 1984, 1（3）：4-0.
[4] 邹学校, 周群初, 戴雄泽, 等. 辣椒雄性不育系9704A的选育[J] . 湖南农业科学, 2000（5）：39-40.
[5] Hu J, Huang W, Huang Q, et al. Mitochondria and cytoplasmic male sterility in plants[J] . Mitochondrion, 2014, 19 Pt B：282-288.
[6] 吴鹤鸣, 佘建明, 周邗扬, 等. 羊角椒雄性不育系及保持系的细胞学观察[J] . 江苏农业学报, 1988（2）：35-38.
[7] 耿三省, 王志源, 蒋健箴, 等. 辣椒雄性不育系小孢子发生的细胞学观察[J] . 园艺学报, 1994（2）：165-169.
[8] 逯红栋, 巩振辉, 王晓敏, 等. 辣椒雄性不育材料小孢子发生的细胞形态学观察[J] . 西北植物学报, 2006, 26（9）：1842-1845.
[9] 彭婧, 巩振辉, 黄炜, 等. 辣椒雄性不育材料H9A小孢子败育机理[J] . 植物学报, 2010, 45（1）：44-51.
[10] 张玲玲, 张晓芬, 陈斌, 等. 辣椒细胞质雄性不育系FS1030A小孢子发生的细胞形态学观察[J] . 华北农学报, 2012, 27（5）：122-126.
[11] Hirose T, Fujime Y. Studies on hybrid seed production using a new male sterility line in C. annuum[J] . Jap Soc Hort Sci, 1981, 50（1）：66-70.
[12] 石太渊, 印东生. 辣椒雄性不育系小孢子发生的细胞形态学及减数分裂观察[J] . 辽宁农业科学, 1999（6）：7-10.
[13] 王述彬, 罗向东, 戴亮芳, 等. 辣（甜）椒细胞质雄性不育系减数分裂和雄配子发生过程[J] . 园艺学报, 2004, 31（6）：807-810.
[14] 王述彬, 罗向东, 戴亮芳, 等. 甜椒胞质雄性不育雄配子发育的解剖学和超微结构研究[J] . 植物科学学报, 2004, 22（6）：557-560.
[15] 李莹莹, 魏佑营, 张瑞华, 等. 辣椒雄性不育系与可育系小孢子发生的细胞学观察[J] . 植物研究, 2006, 26（4）：411-415.
[16] 王兰兰, 王晓林, 魏兵强, 等. 辣椒雄性不育系及保持系小孢子发育的细胞学比较[J] . 西北农业学报, 2015, 24（1）：115-118.
[17] 魏兵强, 张淼, 王兰兰, 等. 辣椒胞质雄性不育及其育性恢复研究进展[J] . 生物技术通报, 2016, 32（4）：1-5.
[18] 孙立全, 霍治军, 常彩涛, 等. 辣椒雄性不育系小孢子发育及脯氨酸等含量的研究[J] . 华北农学报, 2003, 18（4）：39-41.
[19] 刘莉, 蒋苑, 马静, 等. 水稻细胞质雄性不育花药和叶片中的活性氧代谢研究[J] . 中国农学通报, 2016（12）：6-12.
[20] 张子学, 侯喜林. 辣椒细胞质雄性不育与活性氧代谢的关系[J] . 西北植物学报, 2005, 25（4）：799-802.
[21] 李莹莹, 魏佑营, 张瑞华, 等. 辣椒雄性不育“三系”花蕾中3种同工酶活性的动态变化[J] . 中国农学通报, 2005, 21（4）：226-229.
[22] 王永勤, 曹家树, 虞慧芳, 等. 白菜核雄性不育两用系生理生化特性的分析[J] . 园艺学报, 2003, 30（2）：212-214.
[23] Villarreal F, Martín V, Colaneri A, et al. Ectopic expression of mitochondrial gamma carbonic anhydrase 2 causes male sterility by anther indehiscence[J] . Plant Molecular Biology, 2009, 70（4）：471-85.
[24] 吕俊恒, 文锦芬, 莫云容, 等. 辣椒雄性不育系葡萄糖-6-磷酸脱氢酶基因的克隆及表达分析[J] . 西北植物学报, 2015, 35（4）：682-687.
[25] 邓明华, 文锦芬, 赵凯, 等. 辣椒苹果酸脱氢酶基因的表达及其与雄性不育的关系[J] . 园艺学报, 2014, 41（S）：2703.
[26] Touzet P, Meyer EH. Cytoplasmic male sterility and mitochondrial metabolism in plants[J] . Mitochondrion, 2014, 19 Pt B：166-171.
[27] Flavell R. A model for the mechanism of cytoplasmic male sterility in plants, with special reference to maize[J] . Plant Science Letters, 1974, 3（4）：259-263.
[28] Geisler DA, Papke C, Obata T, et al. Downregulation of the delta-subunit reducesmitochondrial ATP synthase levels, alters respiration, and restricts growthand gametophyte development in Arabidopsis[J] Plant Cell, 2012, 24（7）：2792-81.
[29] Wen L, Ruesch KL, Ortega VM, et al. A nuclear restorer-of-fertility mutation disrupts accumulation of mitochondrial ATP synthase subunit α in developing pollen of S male-sterile maize[J] . Genetics, 2003, 165（2）：771-779.
[30] Meyer EH, Tomaz T, Carroll AJ, et al. Remodeled respiration in ndufs4 with low phosphorylation efficiency suppresses Arabidopsis germination and growth and alters control of metabolism at night[J] . Plant Physiology, 2009, 151（2）：603-619.
[31] Robison MM, Ling X, Smid MP, et al. Antisense expression of mitochondrial ATP synthase subunits OSCP（ATP5）and（ATP3）alters leaf morphology, metabolism and gene expression in Arabidopsis[J] . Plant & Cell Physiology, 2009, 50（10）：1840-1850.
[32] 胡适宜, 王摸善, 徐丽云. 小麦雄性不育系与保持系的小孢子发育的电子显微镜研究[J] . 植物学报, 1977, 19（3）：167-171.
[33] 王俐, 祁新红, 刘根齐, 等. 高梁雄性不育系热激前后线粒体的变化与育性的关系[J] . 遗传学报, 2000, 27（9）：834-838.
[34] Horn R. Recombination：Cytoplasmic male sterility and fertility restoration in higher plants[M] // Progress in Botany. Springer Berlin Heidelberg, 2006（67）：31-52.
[35] Hanson MR, Bentolila S. Interactions of mitochondrial and nuclear genes that affect male gametophyte development[J] . Plant Cell, 2004, 16（Suppl）：154-169.
[36] Chase CD. Cytoplasmic male sterility：a window to the world of plant mitochondrial-nuclear interactions[J] . Trends in Genetics Tig, 2007, 23（2）：81-90.
[37] Dewey RE, Timothy DH, Levings CS. A mitochondrial protein associated with cytoplasmic male sterility in the T cytoplasm of maize[J] . Proceedings of the National Academy of Sciences, 1987, 84（15）：5374-5378.
[38] Kim S, Lim H, Park S, et al. Identification of a novel mitochondrial genome type and development of molecular markers for cytoplasm classification in radish（Raphanus sativus L.）[J] . Theoretical and Applied Genetics, 2007, 115（8）：1137-1145.
[39] Yang JH, Liu XY, Yang XD, et al. Mitochondrially-targeted expression of a cytoplasmic male sterility-associated orf220 gene causes male sterility in Brassica juncea[J] . BMC Plant Biology, 2010, 10（1）：231.
[40] Ji JJ, Huang W, Li DW, et al. A CMS-related gene, ψatp6-2, causes iMncreased ATP hydrolysis activity of the mitochondrial F1 F o-ATP synthase and induces male sterility in pepper（Capsicum annuum L.）[J] . Plant Molecular Biology Reporter, 2014, 32（4）：888-899.
[41] Kim DH, Kang JG, Kim S, et al. Identification of coxⅡand atp6 region as associated to CMS in Capsicum annuum by using RFLP and long and accurate PCR[J] . J Kor Soc Hort SCI, 2001, 42：121-127.
[42] Kim DH, Kim BD. Development of SCAR markers for early identification of cytoplasmic male sterility genotype in chili pepper（Capsicum annuum L.）[J] . Mol Cells, 2005, 20（3）：416-422.
[43] Kim DH, Kim BD. The organization of mitochondrial atp6 gene region in male fertile and CMS lines of pepper（Capsicum annuum L.）[J] . Curr Genet, 2006, 49（1）：59-67.
[44] Kim DH, Kang JG, Kim BD. Isolation and characterization of the cytoplasmic male sterility-associated orf456 gene of chili pepper（Capsicum annuum L.）. [J] . Plant Molecular Biology, 2007, 63（4）：519-532.
[45] Horn R, Gupta K J, Colombo N. Mitochondrion role in molecular basis of cytoplasmic male sterility. [J] . Mitochondrion, 2014, 19（3）：198.
[46] Hayward AC, Tollenaere R, Daltonmorgan J, et al. Molecular marker applications in plants. [J] . Methods in Molecular Biology, 2015, 1245：13-27.
[47] 王述彬, 罗向东, 戴亮芳, 等. 辣椒细胞质雄性不育系与其保持系线粒体DNA的RAPD分析[J] . 江苏农业学报, 2008, 24（1）：44-47.
[48] 刘科伟, 王述彬, 刘金兵, 等. 辣椒细胞质雄性不育基因的AFLP分析[J] . 分子植物育种, 2009, 7（4）：736-742.
[49] 魏兵强, 王兰兰, 陈灵芝. 辣椒胞质雄性不育基因的分子标记[J] . 西北农业学报, 2010, 19（10）：166-168.
[50] 袁稳. 辣椒细胞质雄性不育相关基因的克隆及表达研究[D] . 南京：南京农业大学, 2012.
[51] 颜秀娟, 何鑫, 王学梅, 等. 宁夏羊角椒雄性不育基因ISSR的分子标记[J] . 北方园艺, 2015（17）：82-84.
[52] 郭爽, 马宁, 杨文才, 等. 辣椒花器官发育MADS-box基因的克隆与表达分析[J] . 园艺学报, 2010, 37（10）：1591-1597.
[53] 马宁, 鲍顺淑, 连青龙, 等. 辣椒花器官发育相关PAP3基因的克隆与生物信息学分析简[J] . 江苏农业科学, 2016, 44（7）：52-54.
[54] 马宁, 刘辰, 王茹芳, 等. 辣椒PAP3和PPI蛋白相互作用的鉴定[J] . 园艺学报, 2016, 43（3）：564-570.
[55] 林文娟, 彭少丹, 艾丽君, 等. 植物雄性不育的蛋白质组学研究进展[J] . 中国农学通报, 2013（33）：277-280.
[56] Zhang XF, Chen B, Zhang LY, et al. Identification of proteins associated with cytoplasmic male sterility in pepper（Capsicum annuum L.）[J] . South African Journal of Botany, 2015, 100：1-6.
[57] 吕晓菡, 方献平, 柴伟国, 等. 辣椒胞质不育系与保持系花药的细胞学和蛋白质组学差异分析[J] . 浙江大学学报：农业与生命科学版, 2015, 41（1）：44-55.
[58] Liu C, Ning M, Wang PY, et al. Transcriptome sequencing and de novo analysis of a cytoplasmic male sterile line and its near-iso-genic restorer line in chili pepper[J] . PLoS One, 2013, 8（6）：65209.
[59] Balk J, Leaver CJ. The PET1-CMS mitochondrial mutation in sunflower is associated with premature programmed cell death and cytochrome c release[J] . Plant Cell, 2001, 13（8）：1803.
[60] Ji JJ, Huang W, Yin CC, et al. Mitochondrial cytochrome c oxidase and F₁F_o-ATPase dysfunction in peppers（Capsicum annuum L.）with cytoplasmic male sterility and its association with orf507 and Ψatp6-2 genes[J] . International Journal of Molecular Sciences, 2013, 14（1）：1050-1068.
[61] Wan C, Li S, Wen L, et al. Damage of oxidative stress on mitochon-dria during microspores development in Honglian CMS line of rice[J] . Plant Cell Reports, 2007, 26（3）：373-382.
[62] Liu G, Tian H, Huang Y Q, et al. Alterations of mitochondrial protein assembly and jasmonic acid biosynthesis pathway in Honglian（HL）-type cytoplasmic male sterility rice[J] . Journal of Biolo-gical Chemistry, 2012, 287（47）：40051-40060.