Advancements in Quantitative Proteomics Technologies Based on Mass Spectrometry

doi:10.13560/j.cnki.biotech.bull.1985.2017-0343

Abstract

Abstract: Quantitative proteomic analysis is one of the key technologies in proteomics research. With the development of quantitative techniques,quantitative proteomics based on mass spectrometry has become an important branch of proteomics research. Quantitative proteomics technology is generally classified into two categories,untargeted quantitative proteomics and targeted quantitative proteomics. Targeted quantitative technology includes MRM and PRM models,and untargeted quantitative technology includes label-free and in vivo or vitro labeling. The most commonly used isotope labeling reagents are iTRAQ and TMT. According to the data collection model,the quantitative technology can be grouped into DDA and DIA. Through the collection and analysis of relevant literature,this article systematically introduces the main characteristics and current situation of quantitative proteomics based on mass spectrometry,aiming at providing help for the researchers on life science to better apply the quantitative proteomics technologies.

Key words: quantitative proteomics, target quantitative, stable isotope labeling, label-free, post-translational modification

MU Yong-ying,GU Pei-ming,MA Bo,YAN Wen-xiu,WANG Dao-ping,PAN Ying-hong. Advancements in Quantitative Proteomics Technologies Based on Mass Spectrometry[J]. Biotechnology Bulletin, 2017, 33(9): 73-84.

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