Applications and Challenges of Gene Editing in Non-human Primate Models

doi:10.13560/j.cnki.biotech.bull.1985.2017-0891

Abstract

Abstract: There is a pressing urgency in the medical field to establish an effective animal model to study human diseases. Non-human primates,which are evolutionary,physiologically and pathologically closer to humans than any other animal,are thus considered to be excellent animal models for studies of human diseases. With the development of gene manipulation technologies,researchers have successfully created a variety of non-human primate models via gene editing. Yet,there are still some challenges we have to face,such as off-target,mosaic mutants,and low efficiency of gene knock-in in CRISPR/Cas9. In this paper,we summarize the applications of gene editing in non-human primate models,as well as the existing problems and improvements,aimed to provide a reference for further research in non-human primate models mimicking human diseases.

Key words: non-human primate models, TALENs, CRISPR/Cas9, off-target, mosaic mutants

BI Yan-zhen, XIAO Hong-wei, ZHANG Li-ping, REN Hong-yan, HUA Zai-dong, HUA Wen-jun, WANG Zheng, NIU Min-jie, LIN Zheng-yun, REN Xi-dong, SUN Li-hua, ZHENG Xin-min. Applications and Challenges of Gene Editing in Non-human Primate Models[J]. Biotechnology Bulletin, 2018, 34(5): 48-56.

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