Effects of the Characteristic Changes of Mice Hematopoietic Stem/Progenitor Cells on the Aged Immune Imbalance in the Aging Process

doi:10.13560/j.cnki.biotech.bull.1985.2018-0149

Abstract

Abstract: This work is to explore the age-related changes of hematopoietic stem/progenitor cells（HSPCs）in the aging process and to reveal the occurrence and development mechanisms of immune “aging” in the aging process. Flow cytometry was used to measure the cell abundance and proliferation activity of hematopoietic stem cells（HSCs）and its downstream progenitor cells. The results showed that the abundances of HSCs and multipotent progenitor cell（MPP）and granuiocyte/macrophage progenitor（GMPs）cells in bone marrow cells increased significantly with the rise of age,and the number of cells while entering the phase of division（G1,G2/S/M）also increased. In addition,the myeloid-derived suppressor cells（MDSCs）in the downstream cells of GMPs accumulated. In conclusion,the results revealed that HSCs were activated in the aging process,promoting cell proliferation and the differentiation and accumulation of the myeloid suppressor cells,and inhibiting the immune function of the body. This study may provide reference for understanding the decline of immunity in the aging process.

Key words: aging, hematopoietic stem/ progenitor cells, medullary amplification, immunosuppression

ZHANG Li-jie, LI Xiao-yu, CEN You-fei, ZHOU Zu-ping, PU Shi-ming. Effects of the Characteristic Changes of Mice Hematopoietic Stem/Progenitor Cells on the Aged Immune Imbalance in the Aging Process[J]. Biotechnology Bulletin, 2018, 34(8): 199-203.

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