Research Progress on S1 Nuclease-mediated Functional Nucleic Acid Biosensors

doi:10.13560/j.cnki.biotech.bull.1985.2018-0387

Abstract

Abstract: The S1 nuclease is a highly single-stranded and specific endonuclease that degrades single-stranded DNA or RNA under optimal enzymatic conditions to produce single nucleotides or oligonucleotides with 5'-phosphate. It is relatively insensitive to double-stranded DNA,double-stranded RNA,and DNA-RNA hybrids. At present,based on the activity of S1 nuclease for carrying different signal outputs and amplification methods,a series of biosensors have been constructed to detect metal ions,single-stranded nucleotides,amino acids and other substances. Moreover,the S1 nuclease can be applied to the purification of nucleic acid reaction system,the construction of a multi-dimensional gene Library. In this paper,S1 nuclease is introduced from the aspects of structure to functions,and the representative biosensors mediated by S1 nuclease are summarized from the view of establishment and application. Then,based on the different target substances detected,various S1 nuclease-mediated sensors are classified and introduced. Finally,the current research status of S1 nucleases is analyzed,and the future development direction of S1 nuclease-mediated biosensors is prospected.

Key words: S1 nuclease, single-stranded DNA, fluorescence, biosensor

ZHANG Yuan, TIAN Jing-jing, LUO Yun-bo, TIAN Hong-tao, XU Wen-tao. Research Progress on S1 Nuclease-mediated Functional Nucleic Acid Biosensors[J]. Biotechnology Bulletin, 2019, 35(1): 151-160.

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