Physiological Response of Elodea nuttallii to Uranium Stress and Its Enrichment Effect

doi:10.13560/j.cnki.biotech.bull.1985.2018-0773

Abstract

Abstract: In order to detect whether or not E. nuttallii has uranium enrichment capacity,hydroponic solution culture experiments were conducted to study the growth and enrichment characteristics of E. nutallii under different initial uranium concentrations. The experiment results showed that the enrichment of uranium in E. nuttallii increased with the increase of initial uranium concentration,and the uranium enrichment content reached the maximum of 14 mg/kg（DW）while the initial uranium concentration reached 30 mg/L. Meanwhile,the antioxidant enzyme activity was affected significantly,SOD decreased by 86.3%,POD increased by 202.4%,and CAT increased by 291.6%,compared with the control group. The cell membrane of E. nutallii was seriously damaged,MDA content increased by 117.8%. The photosynthetic pigments were affected significantly,chlorophyll-a,chlorophyll-b,and carotenoids decreased respectively by 27.7%,26.7%,and 35.6%;the PSII of E. nutallii was most affected,with fluorescence kinetics index F_v/F_m,PI_ABS,ABS/CS₀,RC/CS₀,TR₀/CS_0,and ET₀/CS₀ decreased by 47.7%,83.8%,68.7%,90.0%,87.7% and 87.3%,respectively;photosynthetic index P_n,G_a,and C_idecreased by 82.1%,20.5%,and 6.4%,respectively,compared with the control group. Therefore,it is concluded that E. nutallii has certain enrichment on uranium in waters and a significant physiological response to uranium.

Key words: uranium stress, Elodea nuttallii, antioxidant enzyme, fluorescence index, enrichment characteristics

YANG Hao, LUO Xue-gang, DING Han-lin, WANG Zhuo. Physiological Response of Elodea nuttallii to Uranium Stress and Its Enrichment Effect[J]. Biotechnology Bulletin, 2019, 35(3): 39-46.

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