Roles of African Swine Fever Virus Structural Proteins in Viral Infection

doi:10.13560/j.cnki.biotech.bull.1985.2018-0968

Abstract

Abstract: African swine fever（ASF）is an acute,lethal,and hemorrhagic porcine infectious disease caused by African swine fever virus（ASFV）which has been caused serious economic losses to the pig industry. ASFV is a double-stranded DNA virus. The viral genome contains between 150 and 167 open reading frames（ORFs）,and encodes for more 200 proteins,around 50 of them are structural proteins. The structural proteins service as the major composition of viral particles,and play important roles in the assembly of virions,viral adsorption,entry and replication. This paper reviews the roles of ASFV structural proteins in viral infection,and provides guidelines for further studies in the structural proteins of ASFV.

Key words: African swine fever virus, structural proteins, pathogenic mechanism, infection

OU Yun-wen, LIU Li-jun, DAI Jun-fei, MA Bing, ZHANG Yong-guang, ZHANG Jie. Roles of African Swine Fever Virus Structural Proteins in Viral Infection[J]. Biotechnology Bulletin, 2019, 35(6): 156-163.

References

[1] Costard S, Mur L, Lubroth J, et al.Epidemiology of African swine fever virus[J]. Virus Res, 2013, 173(1):191-197.
[2] Galindo-Cardiel I, Ballester M, Solanes D, et al.Standardization of pathological investigations in the framework of experimental ASFV infections[J]. Virus Res, 2013, 173(1):180-190.
[3] Lubisi BA, Bastos ADS, Dwarka RM, et al.Molecular epidemiology of African swine fever in east Africa[J]. Arch Virol, 2005, 150
(12):2439-2452.
[4] Woźniakowski G, Kozak E, Kowalczyk A, et al.Current status of African swine fever virus in a population of wild boar in eastern Poland(2014-2015)[J]. Arch Virol, 2016, 161(1):189-195.
[5] Misinzo G, Kwavi DE, Sikombe CD, et al.Molecular characterization of African swine fever virus from domestic pigs in northern Tanzania during an outbreak in 2013[J]. Trop Anim Health Pro, 2014, 46
(7):1199-1207.
[6] Penrith ML, Vosloo W, Jori F, et al.African swine fever virus eradication in Africa[J]. Virus Res, 2013, 173(1):228-246.
[7] José M, Sánchez V, Lina M.African swine fever(ASF):five years around Europe[J]. Vet Microbiol, 2013, 165(1-2):45-50.
[8] Śmietanka K, Woźniakowski, G, Kozak, E, et al.African swine fever epidemic, Poland, 2014-2015[J]. Emerg Infect Dis, 2016, 22(7):1201-1207.
[9] African Swine Fever(ASF)report N°1-6[R]. OIE’s World Animal Health Information Department, 2018.
[10] Ge S, Li J, Fan X, et al.Molecular characterization of African swine fever virus, China[J]. Emerg Infect Dis, 2018, 24(11):2131-2133.
[11] Chapman DAG, Darby AC, Silva MD, et al.Genomic analysis of highly virulent Georgia 2007/1 isolate of African swine fever virus[J]. Emerg Infect Dis, 2011, 17(4):599-605.
[12] Muñoz MR, Galindo I, Cuesta-Geijo MÁ, et al.Host cell targets for African swine fever virus[J]. Virus Res, 2015, 209:118-127.
[13] Galindo I, Cuesta-Geijo MA, Hlavova K, et al.African swine fever virus infects macrophages, the natural host cells, via clathrin- and cholesterol-dependent endocytosis[J]. Virus Res, 2015, 200:45-55.
[14] Linda KD, David AG, Chapman L, et al.African swine fever virus replication and genomics[J]. Virus Res, 2013, 173(1):3-14.
[15] de Villiers EP, Gallardo C, Arias M, et al. Phylogenomic analysis of 11 complete African swine fever virus genome sequences[J]. Virology, 2010, 400(1):128-136.
[16] Alejo A, Matamoros T, Guerra M, et al.A proteomic atlas of the African swine fever virus particle[J]. J Virol, 2018, 92(3):1681-1690.
[17] Catharina K, Jan HF, Günther MK, et al.The intracellular proteome of African swine fever virus[J]. Sci Rep, 2018, 8(1):1-9.
[18] Andrés G, Alejo A, Simon MC, et al.African swine fever virus protease, a new viral member of the SUMO-1-specific protease family[J]. J Bio Chem, 2001, 276(1):780-787.
[19] Alí A, Germán A, María LS.African swine fever virus proteinase is essential for core maturation and infectivity[J]. J Virol, 2003, 77(10):5571-5577.
[20] Salas ML, Andrés G.African swine fever virus morphogenesis[J]. Virus Res, 2013, 173(1):29-41.
[21] Andrés G, Alejo A, Salas J, et al.African swine fever virus polyproteins pp220 and pp62 assemble into the core shell[J]. J Virol, 2002, 76(24):12473-12482.
[22] Gallardo C, Blanco E, Rodríguez JM, et al.Antigenic properties and diagnostic potential of African swine fever virus protein pp62 expressed in insect cells[J]. J Clin Microbiol, 2006, 44(3):950-956.
[23] Heath CM, Windsor M, Wileman T.Membrane association facilitates the correct processing of pp220 during production of the major matrix proteins of African swine fever virus[J]. J Virol, 2003, 77(3):1682-1690.
[24] Suárez C, Salas ML, Rodríguez JM.African swine fever virus polyprotein pp62 is essential for viral core development[J]. J Virol, 2010, 84(1):176-187.
[25] Eulálio A, Nunes CI, Carvalho AL, et al.Two African swine fever virus proteins derived from a common precursor exhibit different nucleocytoplasmic transport activities[J]. J Virol, 2004, 78(18):9731-9739.
[26] Eulálio A, Nunes CI, Salas J, et al.African swine fever virus p37 structural protein is localized in nuclear foci containing the viral DNA at early post-infection times[J]. Virus Res, 2007, 130(1):18-27.
[27] Eulálio A, Nunes CI, Carvalho AL, et al.Nuclear export of African swine fever virus p37 protein occurs through two distinct pathways and is mediated by three independent signals[J]. J Virol, 2006, 80(3):1393-404.
[28] Kolontsov AA, Ustin AV, Shubina NG, et al.Polypeptides p14 and p31 of the African swine fever virus-early proteins located on the membrane of the infected cell[J]. Vopr Virusoli, 1992, 37(3):165-168.
[29] Simón MC, Andrés G, Viñuela E.Polyprotein processing in African swine fever virus:a novel gene expression strategy for a DNA virus[J]. Embo Journal, 1993, 12(7):2977-2987.
[30] Simón MC, Andrés G, Almazán F, et al.Proteolytic processing in African swine fever virus:evidence for a new structural polyprotein, pp62[J]. J Virol, 1997, 71(8):5799-5804.
[31] Hernáez B, Escribano JM, Alonso C.Visualization of the African swine fever virus infection in living cells by incorporation into the virus particle of green fluorescent protein-p54 membrane protein chimera[J]. Virology, 2006, 350(1):1-14.
[32] Rodriguez JM, Garcia ER, Salas ML, et al.African swine fever virus structural protein p54 is essential for the recruitment of envelope precursors to assembly sites[J]. J Virol, 2004, 78(8):4299-4313.
[33] Hernáez B, Gema DG, Mónica GG, et al.The African swine fever virus dynein-binding protein p54 induces infected cell apoptosis[J]. FEBS Lett, 2004, 569:224-228.
[34] Alonso C, Miskin J, Hernáez B, et al.African swine fever virus protein p54 interacts with the microtubular motor complex through direct binding to light-chain dynein[J]. J Virol, 2001, 75(20):9819-9827.
[35] Gallardo C, Mwaengo DM, Macharia JM, et al.Enhanced discrimination of African swine fever virus isolates through nucleotide sequencing of the p54, p72, and pB602L(CVR)genes[J]. Virus Gen, 2009, 38(1):85-95.
[36] Garcíamayoral MF, Rodríguezcrespo I, Bruix M.Structural models of DYNLL1 with interacting partners:African swine fever virus protein p54 and postsynaptic scaffolding protein gephyrin[J]. FEBS Lett, 2011, 585(1):53-57.
[37] Cubillos C, Gómez SS, Moreno N, et al.African swine fever virus serodiagnosis:a general review with a focus on the analyses of African serum samples[J]. Virus Res, 2013, 173(1):159-167.
[38] Barderas MG, Rodríguez F, Gómez PP, et al.Antigenic and immunogenic properties of a chimera of two immunodominant African swine fever virus proteins[J]. Arch Virol, 2001, 146(9):1681-1691.
[39] 曹琛福, 梁云浩, 花群俊, 等. 非洲猪瘟病毒p54蛋白抗原表位预测及鉴定[J]. 中国预防兽医学报, 2014, 36(11):848-851.
[40] Neilan JG, Zsak L, Lu Z, et al.Neutralizing antibodies to African swine fever virus proteins p30, p54, and p72 are not sufficient for antibody-mediated protection[J]. Virology, 2004, 319(2):337-342.
[41] Irene R, María LN, Modesto RR, et al.The African swine fever virus virion membrane protein pE248R is required for virus infectivity and an early postentry event[J]. J Virol, 2009, 83(23):12290-300.
[42] Bruno H, Guerra M, María LS, et al.African swine fever virus undergoes outer envelope disruption, capsid disassembly and inner envelope fusion before core release from multivesicular endosomes[J]. Plos Pathog, 2016, 12(4):1-32.
[43] Senkevich TG, Ojeda S, Townsley A, et al., Poxvirus multiprotein entry-fusion complex[J]. Proc Natl Acad Sci USA, 2005, 102
(51):18572-18577.
[44] Brookes SM, Sun H, Dixon LK, et al.Characterization of African swine fever virion proteins j5R and j13L:immuno-localization in virus particles and assembly sites[J]. J Gen Virol. 1998, 79:1179-1188.
[45] Suárez C, Gutiérrez BJ, Andrés G, et al.African swine fever virus protein p17 is essential for the progression of viral membrane precursors toward icosahedral intermediates[J]. J Virol, 2010, 84(15):7484-7499.
[46] Angulo A, Viñuela E, Alcamí A.Inhibition of African swine fever virus binding and infectivity by purified recombinant virus attachment protein p12[J]. J Virol, 1993, 67(9):5463-5469.
[47] Camacho A, Viñuela E.Protein p22 of African swine fever virus:an early structural protein that is incorporated into the membrane of infected cells[J]. Virology, 1991, 181(1):251-257.
[48] 孙怀昌, Dixon LK, Arkhouse RME .非洲猪瘟病毒j5R膜蛋白的电脑预测和实验证实[J]. 中国病毒学, 1999, 14(3):236-243.
[49] Lithgow P, Takamatsu H, Werling D, et al.Correlation of cell surface marker expression with African swine fever virus infection[J]. Vet Microbiol, 2014, 168(2-4):413-419.
[50] Sánchez EG, Quintas A, Nogal M, et al.African swine fever virus controls the host transcription and cellular machinery of protein synthesis[J]. Virus Res, 2013, 173(1):58-75.
[51] Alexandre L, Malur A, Cornelis P, et al.Identification of a 25-aminoacid sequence from the major African swine fever virus structural protein VP72 recognised by porcine cytotoxic T lymphocytes using a lipoprotein based expression system[J]. J Virol Methods, 1998, 75(1):113-120.
[52] Keita D, Heath L, Albina E.Control of African swine fever virus replication by small interfering RNA targeting the A151R and VP72 genes[J]. Antivir Ther, 2010, 15(5):727-736.
[53] Muangkram Y, Sukmak M, Wajjwalku W.Phylogeographic analysis of African swine fever virus based on the p72 gene sequence[J]. Genet Mol Res, 2015, 14(2):4566-4574.
[54] Chen X, Yang J, Ji Y, et al.Recombinant Newcastle disease virus expressing African swine fever virus protein 72 is safe and immunogenic in mice[J]. Virol Sin, 2016, 31(2):1-10.
[55] 李秋霞, 滕达, 童德文, 等. 非洲猪瘟病毒VP73基因主要抗原表位区的融合原核表达[J]. 中国生物工程杂志, 2010, 30(2):60-65.
[56] 靳雯雯, 杨晓红, 朱碧波, 等. 非洲猪瘟病毒VP73基因在哺乳动物细胞中的表达[J]. 中国预防兽医学报, 2014, 36(7):574-576.
[57] Sastre P, Pérez T, Costa S, et al.Development of a duplex lateral flow assay for simultaneous detection of antibodies against African and Classical swine fever viruses[J]. J Vet Diagn Invest, 2016, 28(5):543-549.
[58] 李倩, 姚淑霞. 非洲猪瘟病毒VP73蛋白的B细胞表位预测
[J]. 农业科学与技术, 2008, 36(1):7680-7682.
[59] Andrés G, Garcíaescudero R, Viñuela E, et al.African swine fever virus structural protein pE120R is essential for virus transport from assembly sites to plasma membrane but not for infectivity[J]. J Virol, 2001, 75(15):6758-6768.
[60] Alfonso P, José I, Quetglas, et al. Protein pE120R of African swine fever virus is post-translationally acetylated as revealed by post-source decay MALDI mass spectrometry[J]. Virus Gen, 2007, 35(1):81-85.
[61] Epifano C, Krijnse LJ, Salas ML, et al.Generation of filamentous instead of icosahedral particles by repression of African swine fever virus structural protein pB438L[J]. J Virol, 2006, 80(23):11456-11466.
[62] Galindo I, Uela EV, Carrascosa AL.Characterization of the African swine fever virus protein p49:a new late structural polypeptide[J]. J Gen Virol, 2000, 81(1):59-65.
[63] Goatley LC, Dixon LK.Processing and localization of the African swine fever virus CD2v transmembrane protein[J]. J Virol, 2011, 85(7):3294-3305.
[64] Jackson PC, Goatley LC, Cox L, et al.The CD2v protein of African swine fever virus interacts with the actin-binding adaptor protein SH3P7[J]. J Gen Virol, 2004, 85(1):119-130.
[65] Rowlands RJ, Duarte MM, Boinas F, et al.The CD2v protein enhances African swine fever virus replication in the tick vector, Ornithodoros erraticus[J]. Virology, 2009, 393(2):319-328.
[66] Burmakina G, Malogolovkin A, Tulman ER, et al.African swine fever virus serotype-specific proteins are significant protective antigens for African swine fever[J]. J Gen Virol, 2016, 97(7):1670-1675.
[67] Sanna G, Dei GS, Bacciu D, et al.Improved strategy for molecular characterization of African swine fever viruses from Sardinia, based on analysis of p30, CD2V and I73R/I329L variable regions[J]. Trans Emerg Dis, 2017, 64(4):1280-1286.
[68] Malogolovkin A, Burmakina G, Tulman ER, et al.African swine fever virus CD2v and C-type lectin gene loci mediate serological specificity[J]. J Gen Virol, 2015, 96(4):866-873.
[69] Galindo I, Viñuela E, Carrascosa AL.Protein cell receptors mediate the saturable interaction of African swine fever virus attachment protein p12 with the surface of permissive cells[J]. Virus Res, 1997, 49(2):193-204.
[70] 陈腾, 张守峰, 周鑫韬, 等. 我国首次非洲猪瘟疫情的发现和流行分析[J]. 中国兽医学报, 2018(9):1831-1832.
[71] Dennis N .Arrival of deadly pig disease could spell disaster for China[J]. Science, 2018, 361(6404):741-741.
[72] 欧云文, 马小元, 王俊, 等. 非洲猪瘟分子病原学及分子流行病学研究进展[J]. 中国兽医学报, 2018(2):416-420.
[73] 欧云文, 阎传忠, 张杰, 等. 非洲猪瘟病毒的分子病原学及致病机理研究进展[J]. 中国畜牧兽医, 2017, 44(7):2139-2146.
[74] 王西西, 陈青, 陈鸿军, 等. 非洲猪瘟病毒免疫逃逸相关蛋白研究进展[J]. 病毒学报, 2018(6):929-935.
[75] 戈胜强, 吴晓东, 张志诚, 等. 非洲猪瘟疫苗研究进展[J]. 畜牧兽医学报, 2016, 47(1):10-15.