Physiological Responses of Soybean to Phenanthrene and Its Tolerance Mechanism

doi:10.13560/j.cnki.biotech.bull.1985.2019-1167

Abstract

Abstract:

In order to investigate the tolerance mechanism and physiological response of plants under phenanthrene stress,foliar application experiment to soybean（Glycine max. L）was conducted,and the effects of different concentrations of phenanthrene to the growth of soybean seedlings,including phenotypic difference,biomass,oxidative damage and antioxidant enzyme activities,were explored. The results showed that the biomass of leaves increased under low concentration of phenanthrene stress（≤50 μmol/L）. Also the content of chlorophyll and carotenoid increased. The content malondialdehyde（MDA）,the activity of proline,superoxide dismutase（SOD）,and peroxidase（POD）were significantly higher than those in control group. Under the concentration of phenanthrene（75-100 μmol/L）,the content of MDA increased along with the increasing of phenanthrene concentration,while the biomass of the soybean seedlings,content of chlorophyll,the content of permeable stress substance,the activity of SOD were inhibited,and the carotenoid content and POD activity in the leaves were high. The above results reveal that plants eliminate the effects of applying phenanthrene in leaves on the biomass of the plants,content of chlorophyll,and oxidative stress by increasing POD and SOD activities as well as the contents of carotenoid and permeable stress substance. In addition,POD and carotenoid maintain high levels under high concentration of phenanthrene,suggesting that they play a more stable role in the corresponding phenanthrene stress.

Key words: soybean, phenanthrene stress, antioxidase, photosynthetic pigments, proline

YANG Dan, WANG Gang, WANG Wu-teng, FAN Ya-jun, XIAO Wei-wei, ZHANG Si-qi, LI Qian, JI Jing. Physiological Responses of Soybean to Phenanthrene and Its Tolerance Mechanism[J]. Biotechnology Bulletin, 2020, 36(10): 1-7.

Figures/Tables 6

References 29

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菲浓度/ （μmol?L^-1）	叶片		根
菲浓度/ （μmol?L^-1）	鲜重/g	干重/g	鲜重/g	干重/g
CK	1.28±0.15 b	0.32±0.07 b	0.94± 0.07 a	0.38±0.08 a
P25	1.49±0.05 a	0.45±0.03 a	0.91±0.05 a	0.39±0.07 a
P50	1.54±0.11 a	0.48±0.07 a	0.85±0.12 a	0.38±0.04 a
P75	1.68±0.05 a	0.55±0.09 a	0.85±0.07 a	0.35±0.07 a
P100	1.05±0.11 c	0.18±0.04 c	0.80±0.06 a	0.32±0.02 a

菲浓度/ （μmol?L^-1）	叶片		根
菲浓度/ （μmol?L^-1）	鲜重/g	干重/g	鲜重/g	干重/g
CK	1.28±0.15 b	0.32±0.07 b	0.94± 0.07 a	0.38±0.08 a
P25	1.49±0.05 a	0.45±0.03 a	0.91±0.05 a	0.39±0.07 a
P50	1.54±0.11 a	0.48±0.07 a	0.85±0.12 a	0.38±0.04 a
P75	1.68±0.05 a	0.55±0.09 a	0.85±0.07 a	0.35±0.07 a
P100	1.05±0.11 c	0.18±0.04 c	0.80±0.06 a	0.32±0.02 a