Biotechnology Bulletin ›› 2020, Vol. 36 ›› Issue (12): 91-97.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0833
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HUANG Yang-tian(), LU Yu-biao, HUANG Yi-tie, MENG Fan-long, XU Kai-wen, LI Peng()
Received:
2020-07-06
Online:
2020-12-26
Published:
2020-12-22
Contact:
LI Peng
E-mail:lpqp3818081@163.com;43677860@163.com
HUANG Yang-tian, LU Yu-biao, HUANG Yi-tie, MENG Fan-long, XU Kai-wen, LI Peng. Screening and Identification of Marine Electricity-producing and Lipase-producing Bacteria and Preliminary Study on Its Culture Conditions[J]. Biotechnology Bulletin, 2020, 36(12): 91-97.
操作步骤 | 空白对照/mL | 样品/mL(3个重复) |
---|---|---|
0.05 mol/L pH8.0 Tris-HCl缓冲溶液 | 5 | 5 |
橄榄油乳化液 | 4 | 4 |
60℃水浴预热5-10 min | ||
95%无水乙醇 | 15 | 0 |
发酵液 | 1.0(灭活) | 1.0 |
60℃水浴、震荡10 min | ||
95%无水乙醇 | 0 | 15 |
1%酚酞 | 0.025 | 0.025 |
0.05 mol/L NaOH溶液滴定至终点 | V空 | V样 |
操作步骤 | 空白对照/mL | 样品/mL(3个重复) |
---|---|---|
0.05 mol/L pH8.0 Tris-HCl缓冲溶液 | 5 | 5 |
橄榄油乳化液 | 4 | 4 |
60℃水浴预热5-10 min | ||
95%无水乙醇 | 15 | 0 |
发酵液 | 1.0(灭活) | 1.0 |
60℃水浴、震荡10 min | ||
95%无水乙醇 | 0 | 15 |
1%酚酞 | 0.025 | 0.025 |
0.05 mol/L NaOH溶液滴定至终点 | V空 | V样 |
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