Extraction of Scutellaria baicalensis Polysaccharides and Its Antioxidant and Antitumor Activities

doi:10.13560/j.cnki.biotech.bull.1985.2020-0764

Abstract

Abstract:

The optimal method of extracting Scutellaria baicalensis Georgi polysaccharides（SBP）from was selected after comparing the extraction rates of SBP by water extraction and alcohol precipitation method,microwave extraction method and ultrasonic extraction method. After the purification of SBP,the ability of SBP scavenging hydroxyl radical and superoxide anion and its total antioxidant capacity were tested by antioxidant assays in vitro. Effects of SBP on the proliferation of gastric cancer cell MGC80-3 was detected by MTT assay. Effects of SBP on the morphology of MGC80-3 cells were evaluated by morphological observation. Then the potential molecular mechanism was explored by Western blot. The results showed that the yield of SBP obtained by microwave extraction was the highest（7.26%）. SBP demonstrated obvious scavenging ability to hydroxyl radical and superoxide anion,and had ideal total antioxidant capacity. SBP effectively inhibited the proliferation of MGC80-3 cells and affected their cell morphologies;SBP at low concentration improved Akt and p-IκB α levels. IKK β and pro-caspase 3 levels,LC3 B/LC3 A ratio and cleave-caspase 3/pro-caspase 3 ratio were positively correlated with SBP concentration. Therefore,SBP presents strong antioxidation and antitumor activities,and has potential value in the development of antioxidants and antitumor drugs.

Key words: Scutellaria baicalensis Georgi, polysaccharides, extraction, antioxidant, antitumor

LI Ping, HU Jian-ran, SHI Bao-zhong, ZHAO Jing-lei. Extraction of Scutellaria baicalensis Polysaccharides and Its Antioxidant and Antitumor Activities[J]. Biotechnology Bulletin, 2021, 37(4): 155-163.

Figures/Tables 8

Fig.1 Hydroxyl radical scavenging capacity of SBP *P<0.05,**P<0.01

Fig.2 Superoxide anion radical scavenging capacity of SBP

Fig.3 T-AOC detection of SBP

Fig.4 Inhibitory effects of SBP on the cell proliferation of MGC80-3 cells

Fig.5 Effects of SBP on the morphology of MGC80-3 cells

Fig.6 Western blot assay

Fig.7 Expression level analysis of associated proteins **P<0.01

Fig.8 Content analysis of active forms of associated proteins **P<0.01

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