Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (6): 295-304.doi: 10.13560/j.cnki.biotech.bull.1985.2020-1502
Previous Articles Next Articles
ZHANG Ying-cai(), HUANG Yue, HAI Yuan, ZHANG Yuan, HU Ya-jie, ZHAO Meng-yi
Received:
2020-12-11
Online:
2021-06-26
Published:
2021-07-08
ZHANG Ying-cai, HUANG Yue, HAI Yuan, ZHANG Yuan, HU Ya-jie, ZHAO Meng-yi. Method Application of Leading Fluorescent Tracers into the Vascular Tissues of Ziziphus jujuba Mill cv. Lingwuchangzao Fruits[J]. Biotechnology Bulletin, 2021, 37(6): 295-304.
Fig.1 Photos showing the introduction method 1 of CFDA A:A cotton thread passes through the short branch phloem from up to down with a fine needle. B:Fix the Eppendorf tube with fine iron wire and keep it upright. C:Fix the cotton thread around the short branch and inject CFDA diluent into the Eppendorf tube with microinjector. D:The Eppendorf tube is closed with the lid and wrapped in silver paper
Fig.2 Photos showing the introduction method 2 of CFDA A:Intertwine the cotton dough around short branch near fruit stalk after rubbing with sandcloth. B:Inject CFDA into the cotton dough with a microinjector after partly sealing up the cotton dough with sealing film. C:Completely seal up the cotton dough with sealing film and applying vaseline on both ends. D:Completely wrap the sealed cotton dough with silver paper
Fig.3 Photos showing the simultaneous introduction meth-ods of CFDA and digitonin A:A cotton thread is passed through the phloem of short branch and the appropriate amount of EDTA into and out of the needle is dripped. B:Fix the Eppendorf tube with a fine iron wire and keep it upright,fix the cotton thread around the short branch,intertwine the cotton dough around the short branch near the Eppendorf tube after rubbing with sandcloth. C:Inject digitonin into the cotton dough with a microinjector after partly sealing up the cotton dough with sealing film,then completely sealing up. D:Inject CFDA diluent into the Eppendorf tube with a microinjector. E:The Eppendorf tube is closed with a lid rapidly after injecting CFDA diluent. F:Seal the cotton dough and completely wrap the Eppendorf tube with silver paper
Fig.4 Photos showing the introduction method of Texas-Red A:Photo showing the introduction of fruit stalk downward Texas-Red during the rapid enlargement period. B:Photo showing the introduction of fruit stalk downward Texas-Red during the maturation period
Fig.5 Vascular bundles characteristic of fruit during the early bulking period A:Vascular bundles of mesocarp. B:Vascular bundles and other structures of mesocarp. C:Endocarp and external vascular bundles. D:Vascular bundle structures of fruit X:Xylem. P:Phloem. VC:Vascular bundle cambium. VB:Vascular bundle. MC:Mucous cavity. CA:Cavity. ME:Mesocarp. EN:Endocarp. SE:Inner epidermis. Bar=50 μm. The same below
Fig.6 Vascular bundles characteristic of fruit during the rapid enlargement period A:Vascular bundles and other structures of mesocarp. B:Vascular bundle structures of fruit. C-D:Vascular bundle structures and branches of fruit
Fig.7 Vascular bundles characteristic of fruit during the coloring period A:Structures of mesocarp and vascular bundles in fruit. B:Branch structures of two vascular bundles of mesocarp. C:Branch structures of multiple vascular bundles of mesocarp. D:Structures connected by multiple vascular bundles
Fig.8 Vascular bundles characteristic of fruit during the maturation period A:Structures of mesocarp and vascular bundles in fruit. B:Vascular bundles structures of mesocarp. C:Branch structures of multiple vascular bundles. D:Branches of vascular bundles and length cutting and cross section structures
Fig.9 Fluorescence tracing map of vascular bundles in jujube fruit A1,A2,and A3 shows vascular bundles fluorography,the corresponding bright field,the overlaid pictures from fluorescence and bright field,respectively after CFDA was introduced into the phloem of the early bulking period fruit for 48 h. B1 andB2 shows vascular bundles fluorography when only CFDA and CFDA and digitonin introduced simultaneously into the phloem of the rapid enlargement period fruit after treatment with 48 h.C1 and C2 shows vascular bundles fluorography when only CFDA and CFDA and digitonin introduced simultaneously into the phloem of the coloring period fruit after treatment with 48 h. D1 and D2 shows vascular bundles fluorography when only CFDA and CFDA and Texas-Red were introduced simultaneously into the maturation period fruit(CF green fluorescence shows the phloem and Texas-Red red fluorescence shows the xylem). X:Xylem. P:Phloem. A1-A3,Bar=100 μm. B1,B2,C1,C2,D1,D2,Bar=250 μm
[1] | 李进伟, 丁绍东, 李苹苹, 等. 五种枣成分及功能研究[J]. 食品工业科技, 2009, 30(7):294-296. |
Li JW, Ding SD, Li PP, et al. Study on composition and function of five cultivars of Chinese jujube[J]. Sci Technol Food Ind, 2009, 30(7):294-296. | |
[2] | 刘鑫, 杨翠花, 雍鹏, 等. 枣成熟期果皮解剖结构特性研究[J]. 山西林业科技, 2013, 42(3):12-14. |
Liu X, Yang CH, Yong P, et al. Study on pericarp anatomical structure of jujuba in mature stage[J]. Shanxi For Sci Technol, 2013, 42(3):12-14. | |
[3] | 边媛, 狄龙, 李定红, 等. 枣果实维管束解剖结构研究[J]. 果树学报, 2015, 32(3):448-452, 523. |
Bian Y, Di L, Li DH, et al. Vascular anatomy of Chinese jujube fruit[J]. J Fruit Sci, 2015, 32(3):448-452, 523. | |
[4] | 刘世鹏, 刘申, 文欣. 枣果肉解剖结构及其裂果性研究[J]. 北方园艺, 2017(14):32-38. |
Liu SP, Liu S, Wen X. Relations between anatomical structure and fruit cracking of jujube fruit[J]. North Hortic, 2017(14):32-38. | |
[5] | 李彦玲, 杨爱珍, 孟泽, 等. 枣果皮组织结构与裂果关系研究[J]. 北京农学院学报, 2016, 31(2):34-41. |
Li YL, Yang AZ, Meng Z, et al. Study on the relationship between jujube peel histological structure and fruit cracking[J]. J Beijing Univ Agric, 2016, 31(2):34-41. | |
[6] | 丁改秀, 王保明, 王小原, 等. 壶瓶枣不同发育期果皮显微结构与裂果的关系[J]. 山西农业科学, 2014, 42(9):948-951. |
Ding GX, Wang BM, Wang XY, et al. Study on the relationship between histological structure and fruit cracking during development in Huping jujube[J]. J Shanxi Agric Sci, 2014, 42(9):948-951. | |
[7] | 黑淑梅, 冯晓东, 常海飞. 枣果实组织结构影响裂果发生的研究进展[J]. 山西农业科学, 2015, 43(7):916-918. |
Hei SM, Feng XD, Chang HF. Research progress in jujube fruit cracking affected by anatomical structure of pericarp[J]. J Shanxi Agric Sci, 2015, 43(7):916-918. | |
[8] | 寇晓虹, 王文生, 吴彩娥, 等. 鲜枣果实解剖结构与其耐藏性关系的研究[J]. 食品科技, 2001, 26(5):67-68. |
Kou XH, Wang WS, Wu CE, et al. Study on the relationship between anatomical structure and storage life of fresh jujube[J]. Food Sci Technol, 2001, 26(5):67-68. | |
[9] |
Braun DM, Wang L, Ruan YL. Understanding and manipulating sucrose phloem loading, unloading, metabolism, and signalling to enhance crop yield and food security[J]. J Exp Bot, 2014, 65(7):1713-1735.
doi: 10.1093/jxb/ert416 URL |
[10] |
Viola R, Roberts AG, Haupt S, et al. Tuberization in potato involves a switch from apoplastic to symplastic phloem unloading[J]. Plant Cell, 2001, 13(2):385-398.
pmid: 11226192 |
[11] |
Oparka KJ, Duckett CM, Prior DAM, et al. Real-time imaging of phloem unloading in the root tip of Arabidopsis[J]. Plant J, 1994, 6(5):759-766.
doi: 10.1046/j.1365-313X.1994.6050759.x URL |
[12] |
Liesche J, Martens HJ, Schulz A. Symplasmic transport and phloem loading in gymnosperm leaves[J]. Protoplasma, 2011, 248(1):181-190.
doi: 10.1007/s00709-010-0239-0 URL |
[13] | Zanon L, Falchi R, Santi S, et al. Sucrose transport and phloem unloading in peach fruit:potential role of two transporters localized in different cell types[J]. Physiol Plant, 2015, 154(2):179-193. |
[14] |
Nie PX, Wang XY, Hu LP, et al. The predominance of the apoplasmic phloem-unloading pathway is interrupted by a symplasmic pathway during Chinese jujube fruit development[J]. Plant Cell Physiol, 2010, 51(6):1007-1018.
doi: 10.1093/pcp/pcq054 URL |
[15] | 侯思皓, 边媛, 牛辉陵, 等. 枣和酸枣果实韧皮部糖分卸载途径及其积累研究[J]. 果树学报, 2017, 34(12):1580-1589. |
Hou SH, Bian Y, Niu HL, et al. Phloem unloading and sugar accumulation in jujube fruits[J]. J Fruit Sci, 2017, 34(12):1580-1589. | |
[16] | 李春丽, 侯柄竹, 张晓燕, 等. 无花果果实韧皮部卸载路径由共质体向质外体途径转变[J]. 科学通报, 2016, 61(8):835-843. |
Li CL, Hou BZ, Zhang XY, et al. A shift of phloem unloading from symplasmic to apoplasmic pathway during fig fruit development[J]. Chin Sci Bull, 2016, 61(8):835-843.
doi: 10.1360/N972015-01056 URL |
|
[17] | 张鹤华, 李艳芳, 聂佩显, 等. 蓝莓果实同化物韧皮部卸载路径与糖代谢酶活性[J]. 林业科学, 2017, 53(3):40-48. |
Zhang HH, Li YF, Nie PX, et al. Phloem unloading pathway of photosynthates and sucrose-metabolizing enzymes activities in Vaccinium corymbosum fruit[J]. Sci Silvae Sin, 2017, 53(3):40-48. | |
[18] | 章英才, 柴雅红, 曹金霞. 灵武长枣果实多糖中单糖组成分析[J]. 干旱地区农业研究, 2018, 36(2):144-152. |
Zhang YC, Chai YH, Cao JX. Monosaccharide composition of polysaccharides in Ziziphus jujuba Mill cv. lingwuchangzao fruit[J]. Agric Res Arid Areas, 2018, 36(2):144-152. | |
[19] |
Clearwater MJ, Luo Z, Ong SE, et al. Vascular functioning and the water balance of ripening kiwifruit(Actinidia chinensis)berries[J]. J Exp Bot, 2012, 63(5):1835-1847.
doi: 10.1093/jxb/err352 pmid: 22155631 |
[20] | 景红霞, 章英才. 灵武长枣果实发育结构特征研究[J]. 广西植物, 2014, 34(4):565-569, 556. |
Jing HX, Zhang YC. Structure characteristic of developmental fruit of Ziziphus jujuba cv. Lingwuchangzao[J]. Guihaia, 2014, 34(4):565-569, 556. | |
[21] | 杨淑娟, 章英才, 郑国琦, 等. 灵武长枣正常果与裂果解剖结构的比较研究[J]. 北方园艺, 2010(22):15-18. |
Yang SJ, Zhang YC, Zheng GQ, et al. Comparative study on dissected structures of normal fruits and cracking ones of Lingwu long-jujube[J]. North Hortic, 2010(22):15-18. | |
[22] |
Chatelet DS, Rost TL, Matthews MA, et al. The peripheral xylem of grapevine(Vitis vinifera)berries. 2. Anatomy and development[J]. J Exp Bot, 2008, 59(8):1997-2007.
doi: 10.1093/jxb/ern061 URL pmid: 18440930 |
[1] | ZHENG Huan, LIN Dong-mei, LIU Jun-yuan, ZHANG Yin-lian, LIN Biao-sheng, LIN Zhan-xi, LI Jing. Analysis of Amino Acid Metabolism Difference Between Fruiting Body and Mycelium of Taiwanofungus camphoratus by LC-QTOF-MS Metabonomics [J]. Biotechnology Bulletin, 2023, 39(5): 254-266. |
[2] | LAI Rui-lian, FENG Xin, GAO Min-xia, LU Yu-dan, LIU Xiao-chi, WU Ru-jian, CHEN Yi-ting. Genome-wide Identification of Catalase Family Genes and Expression Analysis in Kiwifruit [J]. Biotechnology Bulletin, 2023, 39(4): 136-147. |
[3] | ZHANG Le-le, WANG Guan, LIU Feng, HU Han-qiao, REN Lei. Isolation, Identification and Biocontrol Mechanism of an Antagonistic Bacterium Against Anthracnose on Mango Caused by Colletotrichum gloeosporioides [J]. Biotechnology Bulletin, 2023, 39(4): 277-287. |
[4] | CHEN Qiang, ZHOU Ming-kang, SONG Jia-min, ZHANG Chong, WU Long-kun. Identification and Analysis of LBD Gene Family and Expression Analysis of Fruit Development in Cucumis melo [J]. Biotechnology Bulletin, 2023, 39(3): 176-183. |
[5] | MAO Ke-xin, WANG Hai-rong, AN Miao, LIU Teng-fei, WANG Shi-jin, LI Jian, LI Guo-tian. Identification of GRAS Gene Family and Expression Analysis Under Low Temperature Stress in Actinidia chinensis [J]. Biotechnology Bulletin, 2023, 39(11): 297-307. |
[6] | LIU Yuan-yuan, WEI Chuan-zheng, XIE Yong-bo, TONG Zong-jun, HAN Xing, GAN Bing-cheng, XIE Bao-gui, YAN Jun-jie. Characteristics of Class II Peroxidase Gene Expression During Fruiting Body Development and Stress Response in Flammulina filiformis [J]. Biotechnology Bulletin, 2023, 39(11): 340-349. |
[7] | ZHANG Ling, ZHANG Rong-yi, LIU Sheng-ke, TAN Zhi-qiong. Screening of Antagonistic Bacteria for Bacterial Fruit Blotch of Cucurbits and Its Antibacterial Effects [J]. Biotechnology Bulletin, 2023, 39(1): 253-263. |
[8] | SUN Yan, WANG Jin-gang, ZANG Dan-dan, ZHAO Heng-tian, LIU Shu-hua. Transcriptome Analysis of Lonicera caerulea Fruits at Different Developmental Stages [J]. Biotechnology Bulletin, 2022, 38(12): 204-213. |
[9] | MA Qi, LI Ji-lian, XU Shou-zhen, CHEN Hong, LIU Wen-hao, NING Xinzhu, LIN Hai. Genetic Analysis of FBA Trait in Upland Cotton with Major Gene Plus Polygenes Mixed Genetic Model [J]. Biotechnology Bulletin, 2022, 38(10): 148-158. |
[10] | ZHU Hai-yun, MA Yu, KE Yang, LI Bo. Screening and Identification of an Antagonist Against the Pathogen of Kiwifruit Canker and Its Antifungal Activity to the Phytopathogenic Fungus [J]. Biotechnology Bulletin, 2021, 37(6): 66-72. |
[11] | WANG Lu-lu, GENG Xing-min, XU Shi-da. Ethylene Receptor in Fruit Ripening and Flower Senescence [J]. Biotechnology Bulletin, 2021, 37(3): 144-152. |
[12] | LI Ling, YANG Li-xia, GUO Mei. Function of Transcription Factor CNR in the Ripening Process of Tomato Fruit [J]. Biotechnology Bulletin, 2021, 37(2): 51-62. |
[13] | BAI Jin-ming, ZHAO Zi-xiang, QIN Jia-chen, LIU Jian-hui, LIANG Ai-bo. Effects of Tobacco Extracts on the Lifespan of Drosophila melanogaster and Related Gene Transcription [J]. Biotechnology Bulletin, 2020, 36(3): 162-167. |
[14] | YANG Li-juan, LI Shi-fang, LU Mei-guang. miRNA-mediated Regulation Involved in Plant Pathogen [J]. Biotechnology Bulletin, 2020, 36(1): 101-109. |
[15] | ZHANG Yuan-yuan, SHAO Dong-nan, CUI Bai-ming. Construction of Processing Tomato α-Man Mutant Based on CRISPR/Cas9 [J]. Biotechnology Bulletin, 2019, 35(6): 9-16. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||