Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (6): 295-304.doi: 10.13560/j.cnki.biotech.bull.1985.2020-1502

Previous Articles     Next Articles

Method Application of Leading Fluorescent Tracers into the Vascular Tissues of Ziziphus jujuba Mill cv. Lingwuchangzao Fruits

ZHANG Ying-cai(), HUANG Yue, HAI Yuan, ZHANG Yuan, HU Ya-jie, ZHAO Meng-yi   

  1. School of life Sciences,Ningxia University,Yinchuan 750021
  • Received:2020-12-11 Online:2021-06-26 Published:2021-07-08

Abstract:

The fruits of Ziziphus jujuba Mill cv. Lingwuchangzao in different developmental stages were used as experimental materials,the microstructure and change laws of vascular bundles in the fruits of different developmental stages can be clarified using paraffin section method. Based on these results and applying methods of leading fluorescent tracers into vascular tissues of Z. jujuba Mill cv. Lingwuchangzao fruits,the techniques of fluorescent dye tracer in living cell were used to explore the research methods of the unloading path of photosynthates in vascular bundles phloem in the Z. jujuba Mill cv. Lingwuchangzao fruit timely. The results showed that:1)The vascular bundles(especially the vascular bundles nearby endocarp)in the fruit was concentrated and abundant in the early bulking period,the vascular bundles were single or two back-to-back gathered together to be developing. In the rapid enlargement period fruit,the parenchyma cells in the mesocarp developed rapidly and arranged loosely,they gradually increased from the outer to the inner volume,some even arranged into a reticular structure,surrounded by a larger cavity,the number of vascular bundles decreased in the same visual field,and the vascular bundles were mostly two back-to-back,three or four gathered together to be developing. The cell cavities were further enlarged and the number increased in the pericarp in the coloring period,compared with the previous two stages,the number of vascular bundles was fewer in the same visual field,and formed multi-branch structures by multiple vascular bundles. Compared with the coloring period,the cavity space formed by parenchyma cells continued to increase,and the number of vascular bundles did not change significantly,multi-branch structures of multiple vascular bundles were slightly different in the maturation period fruit. 2)By using the techniques of fluorescent dye tracer in living cell,the fluorescent tracer carboxyfluorescein diacetate and plasma membrane penetrating reagent digitonin were led into the phloem of vascular bundles,and the fluorescent tracer Texas-Red were led into the vascular bundles xylem of Z. jujuba Mill cv. Lingwuchangzao fruit in different developmental stages,the fluorescence characteristics were observed by CLSM,the research methods of the phloem unloading path of photosynthates in Z. jujuba Mill cv. Lingwuchangzao fruit were gained,which provides scientific bases for photosynthates accumulation and quality regulation for Z. jujuba Mill cv. Lingwuchangzao fruit.

Key words: Ziziphus jujuba Mill cv. Lingwuchangzao, fruit, vascular tissues, fluorescent tracers, leading into