Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (8): 141-151.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0068

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Identification and Tissue Specific Expression Analysis of AKR Gene Family in Grape

MA Ya-nan1(), LU Xu2, WEI Yun-chun1, LI Kang1, WEI Ruo-nan1, LI Sheng1(), MA Shao-ying3()   

  1. 1. College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070
    2. College of horticulture,Gansu Agricultural University,Lanzhou 730070
    3. Basic Experimental Teaching Center of Gansu Agricultural University,Lanzhou 730070
  • Received:2021-01-17 Online:2021-08-26 Published:2021-09-10
  • Contact: LI Sheng,MA Shao-ying E-mail:m568518983@qq.com;lish@gsau.edu.cn;mashy@gsau.edu.cn

Abstract:

Aldo-Keto Reductase(AKR)is a kind of NADP-dependent oxidoreductase. Through the identification and analysis of grape AKR genes,the aim is to reveal the molecular biological function and genetic regulation mechanism of AKR genes. Based on the latest assembly reference genome data of grape in 2020,bioinformatics was used to analyze the physicochemical properties,gene structure,Motif analysis,cis-acting elements,subcellular localization,secondary structure prediction,and tissue-specific expression analysis of grape AKR genes. Finally,fluorescence quantitative analysis was used to analyze the change of grape AKR gene expressions under salt stress. Thirteen members of AKR gene family were identified at the genome-wide level and divided into 2 subfamilies. Genetic structure analysis demonstrated that the number and location of exons among different members varied. Motif analysis showed that the conserved domains of AKR protein were highly similar. The cis-acting element analysis showed that the upstream promoter elements of this gene not only had core elements and enhancement elements,but also had various hormone and stress response elements,organ specific elements and light regulatory elements. The prediction of secondary structure and subcellular localization indicated that the gene family was mainly expressed in the nucleus,and was dominated by α-helix and irregular coil. Analysis via gene chip expression profile showed that the expression levels of AKR family members were significantly different and had tissue specificity. Fluorescence quantitative results showed that the expression levels of VvAKR02,VvAKR05,VvAKR07,VvAKR11,VvAKR12 and VvAKR13 were up-regulated under salt stress. In conclusion,13 AKR family genes were identified from grape and their expression levels under salt stress were analyzed,which may provide a theoretical and experimental basis for mining salt-tolerant genes in AKR family of grape.

Key words: grape, the AKR gene family, bioinformatics, gene chip expression profile, salt stress