Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (10): 132-139.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1493
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KONG De-zhen(), NIE Ying-bin(), XU Hong-jun, CUI Feng-juan, MU Pei-yuan, TIAN Xiao-ming()
Received:
2021-12-02
Online:
2022-10-26
Published:
2022-11-11
Contact:
NIE Ying-bin,TIAN Xiao-ming
E-mail:kongdezhen1746@163.com;nieyingbin@126.com;txming729@163.com
KONG De-zhen, NIE Ying-bin, XU Hong-jun, CUI Feng-juan, MU Pei-yuan, TIAN Xiao-ming. Effects of Blend Seeding on the Yield,Purity and Yield Advantage of F1 in Three-line Hybrid Wheat[J]. Biotechnology Bulletin, 2022, 38(10): 132-139.
杂交种理论纯度Theo- retical purity of hybrids/% | 混杂恢复系粒数Number of blended restorer lines | 杂交种粒数Hybrid seed number |
---|---|---|
100 | 0 | 100 |
97 | 3 | 97 |
94 | 6 | 94 |
91 | 9 | 91 |
88 | 12 | 88 |
85 | 15 | 85 |
82 | 18 | 82 |
Table 1 Artificial blended restorer line test design
杂交种理论纯度Theo- retical purity of hybrids/% | 混杂恢复系粒数Number of blended restorer lines | 杂交种粒数Hybrid seed number |
---|---|---|
100 | 0 | 100 |
97 | 3 | 97 |
94 | 6 | 94 |
91 | 9 | 91 |
88 | 12 | 88 |
85 | 15 | 85 |
82 | 18 | 82 |
混播比例 Mixed sowing proportion | 出苗率 Emergence ratio/% | ♂ 理论株数 ♂Theoretical plant number | ♂ 收获株数 ♂Harvested Plant number | 小区总产量 Total plot yield/g | ♂ 种子产量/g ♂ Seed yield/g | 杂交种产量 Hybrid yield/g | 比CK增产 Increased rate compared to CK/% | 杂交种比例 Hybrid proportion/% |
---|---|---|---|---|---|---|---|---|
3% | 51 | 36 | 29 | 1180.6 | 100.34 | 1080.26 | -16.19 | 91.50 |
6% | 54 | 72 | 58 | 1836 | 200.68 | 1635.32 | 26.86 | 89.07 |
9% | 56 | 108 | 87 | 2339 | 301.02 | 2037.98 | 58.1 | 87.13 |
12% | 56 | 144 | 117 | 1983 | 404.82 | 1578.18 | 22.43 | 79.59 |
15% | 54 | 180 | 146 | 2376 | 505.16 | 1870.84 | 45.14 | 78.74 |
CK | 53 | 210 | 170 | 1878 | 589 | 1289 | — | 100.00 |
Table 2 Effects of different mixed sowing ratios of male parents in combination 1 on the yield of seed production via mixed sowing and mixed harvesting(2018)
混播比例 Mixed sowing proportion | 出苗率 Emergence ratio/% | ♂ 理论株数 ♂Theoretical plant number | ♂ 收获株数 ♂Harvested Plant number | 小区总产量 Total plot yield/g | ♂ 种子产量/g ♂ Seed yield/g | 杂交种产量 Hybrid yield/g | 比CK增产 Increased rate compared to CK/% | 杂交种比例 Hybrid proportion/% |
---|---|---|---|---|---|---|---|---|
3% | 51 | 36 | 29 | 1180.6 | 100.34 | 1080.26 | -16.19 | 91.50 |
6% | 54 | 72 | 58 | 1836 | 200.68 | 1635.32 | 26.86 | 89.07 |
9% | 56 | 108 | 87 | 2339 | 301.02 | 2037.98 | 58.1 | 87.13 |
12% | 56 | 144 | 117 | 1983 | 404.82 | 1578.18 | 22.43 | 79.59 |
15% | 54 | 180 | 146 | 2376 | 505.16 | 1870.84 | 45.14 | 78.74 |
CK | 53 | 210 | 170 | 1878 | 589 | 1289 | — | 100.00 |
混播比例 Mixing proportion | 出苗率 Emergence ratio/% | ♂收获穗数♂ Harvested spikelets | ♀收获穗数 ♀ Harvested spikelets | F0田间纯度 F0 field purity/% | ♀千粒重 ♀ Weight of 1 000 grains/g | ♂千粒重 ♂ Weight of 1 000 grains/g | 杂交制种产量 Hybrid seed yield/g | 比CK增产 Increase rate compared to CK/% |
---|---|---|---|---|---|---|---|---|
3% | 57.0 | 39 | 3 659 | 98.94 | 43.99 | 39.26 | 166.00 | 40.68 |
6% | 53.4 | 77 | 3 381 | 97.77 | 41.71 | 37.6 | 171.35 | 45.21 |
9% | 61.3 | 134 | 3 364 | 96.17 | 41.35 | 39.54 | 193.60 | 64.07 |
12% | 62.5 | 213 | 3 387 | 94.08 | 40.59 | 39.66 | 291.10 | 146.69 |
CK | 64.8 | 475 | 3 627 | 100.00 | 41.50 | 39.59 | 118.00 | — |
Table 3 Effects of mixing ratio of male and female parents on the seed production yield of hybrid line in combination 2 (2019)
混播比例 Mixing proportion | 出苗率 Emergence ratio/% | ♂收获穗数♂ Harvested spikelets | ♀收获穗数 ♀ Harvested spikelets | F0田间纯度 F0 field purity/% | ♀千粒重 ♀ Weight of 1 000 grains/g | ♂千粒重 ♂ Weight of 1 000 grains/g | 杂交制种产量 Hybrid seed yield/g | 比CK增产 Increase rate compared to CK/% |
---|---|---|---|---|---|---|---|---|
3% | 57.0 | 39 | 3 659 | 98.94 | 43.99 | 39.26 | 166.00 | 40.68 |
6% | 53.4 | 77 | 3 381 | 97.77 | 41.71 | 37.6 | 171.35 | 45.21 |
9% | 61.3 | 134 | 3 364 | 96.17 | 41.35 | 39.54 | 193.60 | 64.07 |
12% | 62.5 | 213 | 3 387 | 94.08 | 40.59 | 39.66 | 291.10 | 146.69 |
CK | 64.8 | 475 | 3 627 | 100.00 | 41.50 | 39.59 | 118.00 | — |
混种比例Mixing proportion | ♀粒数 ♀Grain number | ♂粒数 ♂Grain number | 总粒数 Total grain number | F1种子纯度F1 seed purity/% | 制种产量Hybrid seed yield/kg |
---|---|---|---|---|---|
3% | 87 816 | 1 794 | 89 610 | 97.99 | 3.83 |
4% | 85 078 | 2 217 | 87 295 | 97.46 | 3.83 |
5% | 84 412 | 3 105 | 87 517 | 96.45 | 3.84 |
6% | 81 144 | 3 542 | 84 686 | 95.82 | 3.62 |
9% | 80 736 | 6 164 | 86 900 | 92.91 | 3.71 |
12% | 81 288 | 9 798 | 91 086 | 89.24 | 3.89 |
CK 3♂∶9♀ | 87 048 | 100.00 | 3.61 |
Table 4 Seed production purity and seed yield of the sterile line of combination 2 calculated based on the 60% natural outcrossing rate and the number of ears harvested in the experiment
混种比例Mixing proportion | ♀粒数 ♀Grain number | ♂粒数 ♂Grain number | 总粒数 Total grain number | F1种子纯度F1 seed purity/% | 制种产量Hybrid seed yield/kg |
---|---|---|---|---|---|
3% | 87 816 | 1 794 | 89 610 | 97.99 | 3.83 |
4% | 85 078 | 2 217 | 87 295 | 97.46 | 3.83 |
5% | 84 412 | 3 105 | 87 517 | 96.45 | 3.84 |
6% | 81 144 | 3 542 | 84 686 | 95.82 | 3.62 |
9% | 80 736 | 6 164 | 86 900 | 92.91 | 3.71 |
12% | 81 288 | 9 798 | 91 086 | 89.24 | 3.89 |
CK 3♂∶9♀ | 87 048 | 100.00 | 3.61 |
杂种理论纯度 Theoretical purity of hybrid/% | 25粒混恢复系粒数理论值Theoretical value of 25 mixed restorer lines | 显恢复系带粒数实际值Actual value of restorer stripe |
---|---|---|
100 | 0 | 0 |
97 | 0.75 | 1 |
94 | 1.5 | 3 |
91 | 2.25 | 3 |
88 | 3 | 3 |
85 | 3.75 | 4 |
82 | 4.5 | 6 |
回归方程Regression equation y =0.285 7+1.142 8x 相关系数Correlation coefficient r = 0.948 6 * * |
Table 5 Molecular marker purity analysis of restorer mixed with different proportions of hybrid seeds
杂种理论纯度 Theoretical purity of hybrid/% | 25粒混恢复系粒数理论值Theoretical value of 25 mixed restorer lines | 显恢复系带粒数实际值Actual value of restorer stripe |
---|---|---|
100 | 0 | 0 |
97 | 0.75 | 1 |
94 | 1.5 | 3 |
91 | 2.25 | 3 |
88 | 3 | 3 |
85 | 3.75 | 4 |
82 | 4.5 | 6 |
回归方程Regression equation y =0.285 7+1.142 8x 相关系数Correlation coefficient r = 0.948 6 * * |
Fig. 3 Electrophoretic diagram of blend 6% restorer line detected by Wmc474 primer M:Marker1. 1:Hybrid seed. 2:Restorer 99AR144-1. 3-27:Hybrid samples
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