Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (11): 162-174.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0321
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CAO Ying-hui1(), HU Mei-juan1, TONG Yan1, ZHANG Yan-ping1, ZHAO Kai2, PENG Dong-hui1, ZHOU Yu-zhen1()
Received:
2022-03-14
Online:
2022-11-26
Published:
2022-12-01
Contact:
ZHOU Yu-zhen
E-mail:cyhfred0078@163.com;zhouyuzhencn@163.com
CAO Ying-hui, HU Mei-juan, TONG Yan, ZHANG Yan-ping, ZHAO Kai, PENG Dong-hui, ZHOU Yu-zhen. Identification of the ABC Gene Family and Expression Pattern Analysis During Flower Development in Cymbidium ensifolium[J]. Biotechnology Bulletin, 2022, 38(11): 162-174.
基因名称Gene name | 上游引物Forward primer(5'-3') | 下游引物 Reverse primer(5'-3') |
---|---|---|
CeABCB6 | GGAGCTACCCTTGTTCGAGA | TGGACTCTGAGCCTTGTGAG |
CeABCB30 | AGGCCCTTCGTAACCTTTCA | CAAACTTACGCACGACCACA |
CeABCG3 | AGGGAAAGAGCAGCAGGAAT | TGGCAGCTGTCCATTCAAAC |
CeABCG54 | ATCAAGCACACAAGGCCAAC | AAGGTGAGGGAAAGTGGCAT |
CeABCI7 | TGGGGAAGGATATCAGCGAC | CCATGTGCTTGCCTCCAAAT |
TUB | GCAGTTTACGGCGATGTTCA | ACTCTTCCTCGTCAGCTGTG |
Table 1 Primers used for quantitative real-time PCR
基因名称Gene name | 上游引物Forward primer(5'-3') | 下游引物 Reverse primer(5'-3') |
---|---|---|
CeABCB6 | GGAGCTACCCTTGTTCGAGA | TGGACTCTGAGCCTTGTGAG |
CeABCB30 | AGGCCCTTCGTAACCTTTCA | CAAACTTACGCACGACCACA |
CeABCG3 | AGGGAAAGAGCAGCAGGAAT | TGGCAGCTGTCCATTCAAAC |
CeABCG54 | ATCAAGCACACAAGGCCAAC | AAGGTGAGGGAAAGTGGCAT |
CeABCI7 | TGGGGAAGGATATCAGCGAC | CCATGTGCTTGCCTCCAAAT |
TUB | GCAGTTTACGGCGATGTTCA | ACTCTTCCTCGTCAGCTGTG |
Fig. 4 Phylogenetic analysis of ABC gene family in C. ensifolium,O. sativa and A. thaliana The red font refers to C. ensifolium ABC transporter proteins,the blue font refers to O. sativa ABC transporter proteins,the black font refers to A. thaliana ABC transporter proteins
基因名称Gene name | 基因名称Gene name | 复制类型Duplication type | 非同义替换率Ka | 同义替换率Ks | 非同义替换率/同义替换率Ka/Ks |
---|---|---|---|---|---|
CeABCG31 | CeABCG29 | 片段复制 | 0.148 156 999 | 0.608 282 47 | 0.243 566 117 |
CeABCG19 | CeABCG25 | 片段复制 | 0.368 169 066 | NaN | NaN |
CeABCB18 | CeABCB16 | 片段复制 | 0.069 986 027 | 0.674 387 106 | 0.103 777 231 |
CeABCG10 | CeABCG46 | 串联复制 | 0.017 153 167 | 0.023 289 543 | 0.736 517 994 |
CeABCG5 | CeABCG8 | 串联复制 | 0.108 286 131 | 0.156 200 883 | 0.693 249 159 |
CeABCG8 | CeABCG50 | 串联复制 | 0.016 788 187 | 0.022 178 207 | 0.756 967 701 |
CeABCG47 | CeABCG1 | 串联复制 | 0.207 255 225 | 2.066 524 894 | 0.100 291 666 |
CeABCC4 | CeABCC3 | 串联复制 | 0.212 818 781 | 1.459 333 628 | 0.145 832 849 |
CeABCG18 | CeABCG21 | 串联复制 | 0.006 383 502 | 0.021 424 752 | 0.297 949 888 |
CeABCI2 | CeABCI3 | 串联复制 | 0.005 878 541 | NaN | NaN |
CeABCC1 | CeABCC5 | 串联复制 | 0.291 628 981 | NaN | NaN |
CeABCB12 | CeABCB21 | 串联复制 | 0.024 644 867 | 0.050 781 874 | 0.485 308 334 |
CeABCB17 | CeABCB16 | 串联复制 | 0.644 437 783 | NaN | NaN |
CeABCB10 | CeABCB31 | 串联复制 | 0.039 511 484 | 0.03 561 5 | 1.109 405 7 |
CeABCG30 | CeABCG39 | 串联复制 | 0.106 510 279 | 0.544 293 256 | 0.195 685 466 |
CeABCG24 | CeABCG27 | 串联复制 | 0.040 625 407 | 0.058 053 477 | 0.699 792 83 |
CeABCG32 | CeABCG44 | 串联复制 | 0.064 248 894 | 0.099 652 01 | 0.644 732 544 |
Table 2 Nonsynonymous and synonymous substitution ratio calculation of ABC hologous genes in C. ensifolium
基因名称Gene name | 基因名称Gene name | 复制类型Duplication type | 非同义替换率Ka | 同义替换率Ks | 非同义替换率/同义替换率Ka/Ks |
---|---|---|---|---|---|
CeABCG31 | CeABCG29 | 片段复制 | 0.148 156 999 | 0.608 282 47 | 0.243 566 117 |
CeABCG19 | CeABCG25 | 片段复制 | 0.368 169 066 | NaN | NaN |
CeABCB18 | CeABCB16 | 片段复制 | 0.069 986 027 | 0.674 387 106 | 0.103 777 231 |
CeABCG10 | CeABCG46 | 串联复制 | 0.017 153 167 | 0.023 289 543 | 0.736 517 994 |
CeABCG5 | CeABCG8 | 串联复制 | 0.108 286 131 | 0.156 200 883 | 0.693 249 159 |
CeABCG8 | CeABCG50 | 串联复制 | 0.016 788 187 | 0.022 178 207 | 0.756 967 701 |
CeABCG47 | CeABCG1 | 串联复制 | 0.207 255 225 | 2.066 524 894 | 0.100 291 666 |
CeABCC4 | CeABCC3 | 串联复制 | 0.212 818 781 | 1.459 333 628 | 0.145 832 849 |
CeABCG18 | CeABCG21 | 串联复制 | 0.006 383 502 | 0.021 424 752 | 0.297 949 888 |
CeABCI2 | CeABCI3 | 串联复制 | 0.005 878 541 | NaN | NaN |
CeABCC1 | CeABCC5 | 串联复制 | 0.291 628 981 | NaN | NaN |
CeABCB12 | CeABCB21 | 串联复制 | 0.024 644 867 | 0.050 781 874 | 0.485 308 334 |
CeABCB17 | CeABCB16 | 串联复制 | 0.644 437 783 | NaN | NaN |
CeABCB10 | CeABCB31 | 串联复制 | 0.039 511 484 | 0.03 561 5 | 1.109 405 7 |
CeABCG30 | CeABCG39 | 串联复制 | 0.106 510 279 | 0.544 293 256 | 0.195 685 466 |
CeABCG24 | CeABCG27 | 串联复制 | 0.040 625 407 | 0.058 053 477 | 0.699 792 83 |
CeABCG32 | CeABCG44 | 串联复制 | 0.064 248 894 | 0.099 652 01 | 0.644 732 544 |
Fig. 6 Collinearity analysis of ABC genes between C. ensifolium and A. thaliana,O. sativa,P. aphrodite Red line indicates the collinearity of pair of ABC genes
Fig. 8 Expression profiles of ABC genes of C. ensifolium in flower development A:Expression patterns related to ABC subfamilies. B:Hierarchical clusters based on gene expression. ZL:Budding flower. CK:Half flowering stage. SK:Full bloomed stage. SB:Senescence stage
Fig. 9 Express analysis of CeABC genes during flowering development XL:Small bud stage. ZL:Medium bud stage. DL:Big bud stage. CK:Half flowering stage. SK:Full bloomed stage. SB:Senescence stage
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