Expressions of Genes Related to Polyamine Metabolism and Muscle Development in Muscle Tissues of Duck at Different Ages

doi:10.13560/j.cnki.biotech.bull.1985.2021-1303

Abstract

Abstract:

In order to study and clarify the expression changes of genes related to polyamine metabolism and muscle development in ducks’ muscle tissue,high performance liquid chromatography and RT-qPCR method were used to detect the polyamine contents and the expressions of polyamine metabolism and muscle development-related genes in the breast and leg muscle tissues during duck postnatal stage on day 0 to 120,and then to analyze the correlation between the expressions of muscle development-related gene and polyamine metabolism. Results showed that the contents of putrescine,spermidine and spermine in the duck breast and leg muscle at 0 days were the highest,and the polyamine metabolism genes in the breast and leg muscle tissue showed regular changes,respectively. In pectoral muscle tissue,the expressions of MyoD1 and Myf5 were the highest in 0 days,MyoG and IGF-1 were the highest at 30 days,and MSTN had the highest expression at 90 days,and MyoD1 and Myf5 genes were significantly and positively correlated with SPMS,SPDS,SSAT,and SMO genes. In leg muscle,ODC was significantly and positively correlated with MyoD1 and MSTN gene. The above results suggest that the changes in polyamine content,polyamine metabolism and muscle development-related gene expression in duck muscle tissues present a certain similarity,so as to provide the reference for studying polyamine regulating duck muscle development action mechanisms.

Key words: polyamine, metabolism, muscle development related genes, duck

ZHOU Xue-min, KANG Li-juan, GUO Yong-ni, YANG Xiao-han, JIANG Yi-long, WANG Ze-long, SUN Qian, KANG bo. Expressions of Genes Related to Polyamine Metabolism and Muscle Development in Muscle Tissues of Duck at Different Ages[J]. Biotechnology Bulletin, 2022, 38(8): 244-251.

Figures/Tables 9

References 33

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基因 Gene		相关系数 Correlation coefficient	P值 P value
MyoD1	SPMS	0.733 24	6.827 11×10^-4
MyoD1	SPDS	0.846 36	3.594 06×10^-7
MyoD1	SSAT	0.810 69	5.133 09×10^-7
MyoD1	SMO	0.820 04	1.720 74×10^-5
Myf5	SPMS	0.946 4	7.152 42×10^-10
Myf5	SPDS	0.827 96	3.259×10^-7
Myf5	SSAT	0.945 63	0.001 08
Myf5	SMO	0.916 11	3.682 54×10^-8

基因 Gene		相关系数 Correlation coefficient	P值 P value
MyoD1	SPMS	0.733 24	6.827 11×10^-4
MyoD1	SPDS	0.846 36	3.594 06×10^-7
MyoD1	SSAT	0.810 69	5.133 09×10^-7
MyoD1	SMO	0.820 04	1.720 74×10^-5
Myf5	SPMS	0.946 4	7.152 42×10^-10
Myf5	SPDS	0.827 96	3.259×10^-7
Myf5	SSAT	0.945 63	0.001 08
Myf5	SMO	0.916 11	3.682 54×10^-8

基因 Gene		相关系数 Correlation coefficient	P值 P value
MyoD1	ODC	0.746 32	0.005 53
MSTN	ODC	0.919 39	4.878 05×10^-12

基因 Gene		相关系数 Correlation coefficient	P值 P value
MyoD1	ODC	0.746 32	0.005 53
MSTN	ODC	0.919 39	4.878 05×10^-12