Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (9): 207-214.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1450
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LI Wen-shuo(), WANG Lin-song, DU Gui-cai, GUO Qun-qun, ZHANG Ting-ting, YANG Hong LI Rong-gui
Received:
2021-11-21
Online:
2022-09-26
Published:
2022-10-11
LI Wen-shuo, WANG Lin-song, DU Gui-cai, GUO Qun-qun, ZHANG Ting-ting, YANG Hong LI Rong-gui. Gene Cloning of an Aldehyde Dehydrogenase from Bursaphelenchus xylophilus and Biochemical Characterization[J]. Biotechnology Bulletin, 2022, 38(9): 207-214.
引物名称 Name of primer | 序列Sequence(5'-3') |
---|---|
F1 | aaaCATATGggacagctccagagtttggt |
R1 | aaaGGATCCtcactcgatggagatccgctttc |
Table 1 PCR primers used in this study
引物名称 Name of primer | 序列Sequence(5'-3') |
---|---|
F1 | aaaCATATGggacagctccagagtttggt |
R1 | aaaGGATCCtcactcgatggagatccgctttc |
Fig. 3 Expression and purification of the recombinant alde-hyde dehydrogenase M:Protein marker;1:total proteins of E. coli BL21(DE3);2:total proteins of E. coli BL21(DE3)harboring pET-15b-aldh;3:the purified recombinant aldehyde dehydrogenase
Fig. 4 Km of the recombinant aldehyde dehydrogenase calculated by Lineweaver-Burk plot The error line in the figure refers to the standard deviation. The lowercase letters indicate that the difference among different test reached a significant level(P <0.05). The same meaning is suitable for the rest of this paper
Fig.9 Multiple sequences alignments of primary structures of aldehyde dehydrogenases from different sources Bursaphelenchus xylophilus:GenBank NO.CAD5152421.1;Bursaphelenchus okinawaensis:GenBank NO.CAD5218566.1;Trypanosoma grayi:GenBank NO.XP_009312368.1;Caenorhabditis elegans:GenBank NO. NP_741553.1;Mus musculus:GenBank NO.NP_001318043.1;Strongyloides ratti:GenBank NO. XP_024502805.1;Pseudomonas sp. HR199:GenBank NO. CAA06926.1;Schistocerca gregaria:GenBank NO.QVD39485.1;Meloidogyne graminicola:GenBank NO. KAF7633361.1;Ancylostoma caninum:GenBank NO.RCN51637.1;Micrococcus luteus NCTC 2665:GenBank NO. ACS29670.1
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