Regulation of Fusarium wilt Resistance in Cotton by Exogenous Melatonin

doi:10.13560/j.cnki.biotech.bull.1985.2022-0419

Abstract

Abstract:

To investigate the effect and mechanism of exogenous melatonin on cotton resistance against Fusarium wilt, Gossypium barbadense Xinhai 14 treated with foliar application of various concentrations of melatonin（0, 10, 25, 50, 75 and 100 μmol/L）was used to identify disease resistance, to identify the activity of disease resistance related enzymes, and to analyze gene expression in cotton seedlings after inoculation with Fusarium oxysporum f.sp. vasinfectum（Fov）. The results showed that exogenous melatonin did not inhibit the growth of cotton blight, and they 10-100 μmol/L all increased the cotton resistance to Fov to some extent, the best with 50 μmol/L melatonin. Compared with control, 50 μmol/L melatonin pretreatment reduced the hydrogen peroxide（H₂O₂）content and superoxide anion（O₂^·-）production rate in the cotton leaves after inoculation, and enhanced the expressions of peroxidase（POD）, catalase（CAT）, superoxide dismutase（SOD）, ascorbate peroxidase（APX）, chitinase（CHT）, β-1,3 glucanase（GLU）, polyphenol oxidase（PPO）, phenylalanine ammonia lyase（PAL）activities, and significantly increased the expressions of key genes of the lignin synthesis pathway（GbPAL, Gb4CL and GbCAD）and key genes of the flavonoid synthesis pathway（GbCHI, GbDFR and GbTT7）. This showed that 50 μmol/L melatonin treatment may improve the antioxidant capacity of cotton after inoculation, enhance the activities of defense enzymes, regulate the expressions of genes related to the lignin and flavonoid synthesis pathways, and thus improve the resistance of cotton to Fusarium wilt.

Key words: melatonin, Fusarium wilt, cotton, antioxidant enzymes, disease resistance

ZHU Jin-cheng, YANG Yang, LOU Hui, ZHANG Wei. Regulation of Fusarium wilt Resistance in Cotton by Exogenous Melatonin[J]. Biotechnology Bulletin, 2023, 39(1): 243-252.

Figures/Tables 6

Table 1 Primer sequences for quantitative PCR

引物名称Primer name	引物序列Primer sequence（5'-3'）
引物名称Primer name	正向Forward（F）	反向Reverse（R）
GbPAL-F/R	CCAATGGTGAACTCCACCCGTC	TCGTCGGCGTAGGCATAGAGAT
Gb4CL-F/R	CGACGTCGTAGCGCTTCCTTAT	CTCGTGACCAAACCTTTGTGCG
GbCAD-F/R	CCCGAGAAACTAGCACCAGAGC	GCCGCATTAGAGCTCACAAGGA
GbCHI-F/R	CAATGACTGTGGAGCCGACA	CCCAGAGATGATGATTCAGTGG
GbDFR-F/R	GTTCCCACCAAGTTCAAAGATG	GAAGCAGTCCCTTTTCTCGG
GbTT7-F/R	GCTTGCGAGATCAATGGCTACT	CAGCACCAAATGGAATCAACTC
GhUBQ7-F/R	GAAGGCATTCCACCTGACCAAC	CTTGACCTTCTTCTTCTTGTGCTTG

Fig. 1 Effects of exogenous melatonin pretreatment at different concentration on the cotton resistance to Fusarium oxysporum f.sp. vasinfectum（Fov） A: Disease phenotypes of cotton with different treatments at 22 d. B: Disease phenotypes in stems of cotton treated with different treatments at 22 d. C: Cotton disease index at different time points of plants treated with different treatments. Different letters indicate significant difference（P <0.05）, the same below

Fig. 2 Effects of exogenous melatonin at different concentration on the growth of Fov A: Fov colonies 7 d after inoculation on PDA medium. B: Fov colony diameter 7 d after inoculation on PDA medium

Fig. 3 Effects of melatonin pretreatment on cotton antioxidant system after Fov inoculated * indicates significant difference（P < 0.05）, ** indicates extremely significant difference（P < 0.01）, the same below

Fig. 4 Effects of melatonin pretreatment on the cotton defense enzyme activity after Fov inoculated

Fig. 5 Effects of melatonin pretreatment on the cotton expressions of genes related to phenylpropane metabolic pathway related genes after Fov inoculated

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