Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (11): 308-317.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0096
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ZHANG Yu-juan1(), LI Dong-hua2, GONG Hui-hui1, CUI Xin-xiao1, GAO Chun-hua1, ZHANG Xiu-rong1, YOU Jun2(), ZHAO Jun-sheng1()
Received:
2023-02-08
Online:
2023-11-26
Published:
2023-12-20
Contact:
YOU Jun, ZHAO Jun-sheng
E-mail:723949246@qq.com;junyou@caas.cn;zhaojunshengsd@163.com
ZHANG Yu-juan, LI Dong-hua, GONG Hui-hui, CUI Xin-xiao, GAO Chun-hua, ZHANG Xiu-rong, YOU Jun, ZHAO Jun-sheng. Cloning and Salt-tolerance Analysis of NAC Transcription Factor SiNAC77 from Sesamum indicum L.[J]. Biotechnology Bulletin, 2023, 39(11): 308-317.
引物名称Primer name | 引物序列Primer sequence(5'-3') | 用途Purpose |
---|---|---|
NAC77-BamH I-F | tgtctagactcgagggatccATGGAACAGTTGGCCGGTTTTG | 基因克隆Gene clone |
NAC77-BamH I-R | agcctgcagccatggggatccTCAGTAGTTCCACAAGCAGTC | |
35s-F | GACGCACAATCCCACTATCC | 转基因植株检测Transgenic plant detection |
NAC77-R | TCAGTAGTTCCACAAGCAGTC | |
Actin-F | CGGCGAGTCATCGTCATCAATGT | 拟南芥内参引物Internal primer of Arabidopsis thaliana |
Actin-R | GTTCGGGATTACGGTGGTGGGA | |
NAC77-Fq | CTCCACTCACGGACTTGTCA | 荧光定量PCR引物Fluorescent quantitative PCR primer |
NAC77-Rq | AGGAAAGGAGGGTTGGATTG |
Table 1 Primer sequence used in this study
引物名称Primer name | 引物序列Primer sequence(5'-3') | 用途Purpose |
---|---|---|
NAC77-BamH I-F | tgtctagactcgagggatccATGGAACAGTTGGCCGGTTTTG | 基因克隆Gene clone |
NAC77-BamH I-R | agcctgcagccatggggatccTCAGTAGTTCCACAAGCAGTC | |
35s-F | GACGCACAATCCCACTATCC | 转基因植株检测Transgenic plant detection |
NAC77-R | TCAGTAGTTCCACAAGCAGTC | |
Actin-F | CGGCGAGTCATCGTCATCAATGT | 拟南芥内参引物Internal primer of Arabidopsis thaliana |
Actin-R | GTTCGGGATTACGGTGGTGGGA | |
NAC77-Fq | CTCCACTCACGGACTTGTCA | 荧光定量PCR引物Fluorescent quantitative PCR primer |
NAC77-Rq | AGGAAAGGAGGGTTGGATTG |
Fig. 4 Identification of transgenic Arabidopsis plants A: Identification of T1 transgenic Arabidopsis plants by kanamycin. B: Identification of T1 transgenic Arabidopsis plants, 1-12 indicate transgenic lines, “-” indicates pure water blank control, “+” indicates plasmid control, WT indicates wild type of Arabidopsis plants control, and M indicates DL2000 marker. C: Expression of SiNAC77in transgenic Arabidopsis. OE-1, OE-2, and OE-3 were obtained from T1(4, 5 and 8)transgenic plants. * indicate significant difference at P<0.05 level; **indicates significant difference at P<0.01 level. The same below
Fig. 5 Resistance characteristics of transgenic Arabidopsis plants to salt stress A: Seed germination of transgenic Arabidopsis under salt stress. B: The elongation of the primary root of transgenic Arabidopsis under salt stress. C: Fresh weight of transgenic Arabidopsis under salt stress. D: Phenotypes of wild type and transgenic Arabidopsis plants under salt stress
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