Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (2): 203-210.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0582

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Isolation and Identification of Saline-alkali Tolerant Aspergillus terreus SYAT-1 and Its Activities Against Plant Pathogenic Fungi

WANG Feng-ting(), WANG Yan, SUN Ying, CUI Wen-jing, QIAO Kai-bin, PAN Hong-yu, LIU Jin-liang()   

  1. College of Plant Sciences,Jilin University,Changchun 130062
  • Received:2022-05-09 Online:2023-02-26 Published:2023-03-07

Abstract:

Microorganisms co-exist and evolve in saline environment for a long time and have unique adaptability and biological properties, which make them the first choice in the microbial improvement of saline land. A saline-alkali tolerant fungal strain SYAT-1 was isolated from a typical soda saline soil in Songyuan city, Jilin province. It was identified by morphological characteristics observation and ITS-rDNA sequence analysis. The growth characteristics at different pH, salt tolerance, antagonistic activity against phytopathogenic fungi and potential pathogenicity of the strain were investigated. The results showed that it was identified as Aspergillus terreus and grew under alkaline(pH=7.0-12.0)medium conditions, with an optimal pH range of 7.0-9.0. It grew well on both 0-17.5 mg/mL NaCl PDA medium and 0-4mg/mL NaHCO3 PDA medium. Antagonistic phytopathogenic fungi assay results revealed that strain SYAT-1 presented some inhibitory effect on the test phytopathogenic fungi. Potential pathogenicity test results demonstrated that strain SYAT-1 had no potential pathogenicity to the test plants. In sum, A. terreus strain SYAT-1 belongs to a fungus with high salt tolerance characteristics and suppressing phytopathogenic fungi. This study provides new strain resources and theoretical support for the use of microorganisms to improve saline soils and biological control of plant diseases.

Key words: saline-alkali tolerance, Aspergillus terreus, isolation and identification, pathogenic fungus, inhibition