Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (2): 203-210.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0582
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WANG Feng-ting(), WANG Yan, SUN Ying, CUI Wen-jing, QIAO Kai-bin, PAN Hong-yu, LIU Jin-liang()
Received:
2022-05-09
Online:
2023-02-26
Published:
2023-03-07
WANG Feng-ting, WANG Yan, SUN Ying, CUI Wen-jing, QIAO Kai-bin, PAN Hong-yu, LIU Jin-liang. Isolation and Identification of Saline-alkali Tolerant Aspergillus terreus SYAT-1 and Its Activities Against Plant Pathogenic Fungi[J]. Biotechnology Bulletin, 2023, 39(2): 203-210.
土壤编号 Soil No. | 采样时间 Collection time | 菌株编号 Strain code | 土壤编号 Soil No. | 采样时间 Collection time | 菌株编号 Strain code | |
---|---|---|---|---|---|---|
SY-1 | 2020.8.10 | SYAT-1 | SY-8 | 2019.9.16 | SYAT-4 | |
SYA-2 | SY-9 | 2019.9.16 | SYA-1 | |||
SY-2 | 2020.8.10 | SYAT-3 | SYAO-3 | |||
SY-3 | 2020.8.10 | SYTA-1 | SY-10 | 2019.9.16 | SYAT-2 | |
SYA-5 | SYA-4 | |||||
SY-4 | 2020.8.10 | SYTH-1 | SYAN-1 | |||
SYA-3 | SY-11 | 2019.9.16 | SYTH-2 | |||
SY-5 | 2020.8.10 | SYAO-1 | SYAN-2 | |||
SY-6 | 2020.8.10 | SYTA-3 | SY-12 | 2019.9.16 | SYTA-2 | |
SY-7 | 2020.8.10 | SYA-6 | SYA-6 | |||
SYAO-2 |
Table 1 Collected and isolated fungal strains
土壤编号 Soil No. | 采样时间 Collection time | 菌株编号 Strain code | 土壤编号 Soil No. | 采样时间 Collection time | 菌株编号 Strain code | |
---|---|---|---|---|---|---|
SY-1 | 2020.8.10 | SYAT-1 | SY-8 | 2019.9.16 | SYAT-4 | |
SYA-2 | SY-9 | 2019.9.16 | SYA-1 | |||
SY-2 | 2020.8.10 | SYAT-3 | SYAO-3 | |||
SY-3 | 2020.8.10 | SYTA-1 | SY-10 | 2019.9.16 | SYAT-2 | |
SYA-5 | SYA-4 | |||||
SY-4 | 2020.8.10 | SYTH-1 | SYAN-1 | |||
SYA-3 | SY-11 | 2019.9.16 | SYTH-2 | |||
SY-5 | 2020.8.10 | SYAO-1 | SYAN-2 | |||
SY-6 | 2020.8.10 | SYTA-3 | SY-12 | 2019.9.16 | SYTA-2 | |
SY-7 | 2020.8.10 | SYA-6 | SYA-6 | |||
SYAO-2 |
Fig. 2 Phylogenetic tree constructed based on ITS-rDNA nucleotide sequences Bootstrap values(%)> 50 were displayed. The individual strain in the system tree was represented as:GenBank number-Strain name-Strain number
Fig. 6 Antagonistic effect of strain SYAT-1 against diffe-rent pathogenic fungi (a):Sclerotinia sclerotiorum;(b):Fusarium graminearum;(c):Botrytis cinerea;(d):Cochliobolus heterostrophus;(e):Setosphaeria turcica;(f):Magnaporthe oryzae
植物病原真菌Plant pathogenic fungus | 抑菌率Inhibition rate/% |
---|---|
Sclerotinia sclerotiorum | 34.12±0.81d |
Fusarium graminearum | 35.29±0.69d |
Botrytis cinerea | 42.35±0.65c |
Cochliobolus heterostrophus | 47.89±0.33b |
Setosphaeria turcica | 50.59±0.74a |
Magnaporthe oryzae | 21.57±0.73e |
Table2 Inhibition rates of strain SYAT-1 against six plant pathogenic fungi
植物病原真菌Plant pathogenic fungus | 抑菌率Inhibition rate/% |
---|---|
Sclerotinia sclerotiorum | 34.12±0.81d |
Fusarium graminearum | 35.29±0.69d |
Botrytis cinerea | 42.35±0.65c |
Cochliobolus heterostrophus | 47.89±0.33b |
Setosphaeria turcica | 50.59±0.74a |
Magnaporthe oryzae | 21.57±0.73e |
Fig. 7 Potential pathogenicity test of the strain SYAT-1 A:Tobacco,tomato and soybean leaves inoculated with S. sclerotiorum. B:Tobacco,tomato and soybean leaves inoculated with A. terreus SYAT-1. C1,C2:Maize leaves inoculated with spore suspension of S. turcica. C3,C4:Maize leaves inoculated with spore suspension of A. terreus SYAT-1. C5:Maize leaves inoculation with 20% Tween-20 as control
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