QTL Mapping of Sorghum Plant Height and Internode Numbers Based on Super-GBS Technique

doi:10.13560/j.cnki.biotech.bull.1985.2022-1510

Abstract

Abstract:

Plant height and its relative traits are key factors that affect plant type and yield of sorghum. Mapping major QTL for these traits may provide a basis for genetic improvement in sorghum. In this study, a RI population including 205 lines obtained from a cross between American cultivar BTx623 and Guizhou liquor-brewing cultivar Hongyingzi was used to investigate plant height and internode numbers under five environments. The genetic map was constructed based on genotype data from Super-GBS technology, and major QTLs were identified by inclusive composite interval mapping（ICIM）. Totally, eighteen major QTLs were detected on chromosome 1, 3, 4, 8, and 9, of which 7 and 11 QTLs were related to plant height and internode numbers, respectively. Ten major QTLs were repeatedly detected in multiple environments or two traits, of which qPH9.1 was consistent with the known plant high gene Dw1, while qPH1.2, qPH3.2, qIN3.2 and qIN8.1 were colocalized with four homologous genes involving in plant height or internode elongation in rice.

Key words: sorghum, plant height, number of internodes, QTL mapping, candidate genes

XU Jian-xia, DING Yan-qing, FENG Zhou, CAO Ning, CHENG Bin, GAO Xu, ZOU Gui-hua, ZHANG Li-yi. QTL Mapping of Sorghum Plant Height and Internode Numbers Based on Super-GBS Technique[J]. Biotechnology Bulletin, 2023, 39(7): 185-194.

Figures/Tables 7

References 42

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性状 Trait	环境 Environment	亲本Parents		RIL群体RIL population
性状 Trait	环境 Environment	BTx623	红缨子 Hongyingzi	最小值 Minimum	最大值 Maximum	平均值 Mean	标准差 SD	变异系数 CV/%	偏度 Skewness	峰度 Kurtosis
株高 PH/cm	2020GY	120.33	266.33	101.00	360.67	207.37	56.94	27.46	0.176	-0.769
	2020AS	132.67	284.00	106.67	320.33	197.99	47.66	24.07	0.192	-0.773
	2020LD	122.00	196.33	81.67	254.00	178.02	31.65	17.78	-0.213	-0.453
	2021GY	124.33	215.00	105.00	316.67	201.16	43.32	21.53	0.070	-0.513
	2021AS	147.00	238.67	107.33	321.67	196.15	42.99	21.92	0.188	-0.671
节间数 IN	2020GY	8.67	10.67	5.67	13.33	9.73	1.19	12.22	0.144	0.404
	2020AS	7.67	10.33	6.00	12.67	8.75	1.27	14.52	0.509	-0.248
	2020LD	7.00	9.33	4.67	11.00	7.48	0.98	13.07	0.402	1.609
	2021GY	7.00	8.67	5.33	11.00	8.19	0.98	12.02	0.016	0.673
	2021AS	7.00	9.67	5.33	12.67	8.49	1.41	16.64	0.377	-0.087

性状 Trait	环境 Environment	亲本Parents		RIL群体RIL population
性状 Trait	环境 Environment	BTx623	红缨子 Hongyingzi	最小值 Minimum	最大值 Maximum	平均值 Mean	标准差 SD	变异系数 CV/%	偏度 Skewness	峰度 Kurtosis
株高 PH/cm	2020GY	120.33	266.33	101.00	360.67	207.37	56.94	27.46	0.176	-0.769
	2020AS	132.67	284.00	106.67	320.33	197.99	47.66	24.07	0.192	-0.773
	2020LD	122.00	196.33	81.67	254.00	178.02	31.65	17.78	-0.213	-0.453
	2021GY	124.33	215.00	105.00	316.67	201.16	43.32	21.53	0.070	-0.513
	2021AS	147.00	238.67	107.33	321.67	196.15	42.99	21.92	0.188	-0.671
节间数 IN	2020GY	8.67	10.67	5.67	13.33	9.73	1.19	12.22	0.144	0.404
	2020AS	7.67	10.33	6.00	12.67	8.75	1.27	14.52	0.509	-0.248
	2020LD	7.00	9.33	4.67	11.00	7.48	0.98	13.07	0.402	1.609
	2021GY	7.00	8.67	5.33	11.00	8.19	0.98	12.02	0.016	0.673
	2021AS	7.00	9.67	5.33	12.67	8.49	1.41	16.64	0.377	-0.087

性状Trait	2020GY-PH	2020AS-PH	2020SY-PH	2021GY-PH	2021AS-PH	2020GY-IN	2020AS-IN	2020SY-IN	2021GY-IN	2021AS-IN
2020GY-PH	1.000
2020AS-PH	0.832**	1.000
2020SY-PH	0.884**	0.783**	1.000
2021GY-PH	0.860**	0.826**	0.856**	1.000
2021AS-PH	0.844**	0.760**	0.814**	0.861**	1.000
2020GY-IN	0.474**	0.457**	0.387**	0.418**	0.381**	1.000
2020AS-IN	0.399**	0.584**	0.317**	0.383**	0.380**	0.651**	1.000
2020SY-IN	0.431**	0.422**	0.491**	0.428**	0.367**	0.671**	0.500**	1.000
2021GY-IN	0.380**	0.472**	0.302**	0.472**	0.390**	0.684**	0.665**	0.575**	1.000
2021AS-IN	0.475**	0.512**	0.432**	0.515**	0.618**	0.567**	0.633**	0.508**	0.599**	1.000

性状Trait	2020GY-PH	2020AS-PH	2020SY-PH	2021GY-PH	2021AS-PH	2020GY-IN	2020AS-IN	2020SY-IN	2021GY-IN	2021AS-IN
2020GY-PH	1.000
2020AS-PH	0.832**	1.000
2020SY-PH	0.884**	0.783**	1.000
2021GY-PH	0.860**	0.826**	0.856**	1.000
2021AS-PH	0.844**	0.760**	0.814**	0.861**	1.000
2020GY-IN	0.474**	0.457**	0.387**	0.418**	0.381**	1.000
2020AS-IN	0.399**	0.584**	0.317**	0.383**	0.380**	0.651**	1.000
2020SY-IN	0.431**	0.422**	0.491**	0.428**	0.367**	0.671**	0.500**	1.000
2021GY-IN	0.380**	0.472**	0.302**	0.472**	0.390**	0.684**	0.665**	0.575**	1.000
2021AS-IN	0.475**	0.512**	0.432**	0.515**	0.618**	0.567**	0.633**	0.508**	0.599**	1.000

性状 Trait	均方 Mean square			F值F value			广义遗传率 h²_B/%
性状 Trait	基因型Genotype	环境Environment	基因型iro G×E	基因型Genotype	环境Environment	基因型iro G×E	广义遗传率 h²_B/%
株高PH	26 001.18	75 159.27	1 238.43	224.52**	649.01**	10.69**	95.23
节间数IN	14.05	419.71	1.70	43.24**	1 291.97**	5.23**	85.78