Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (7): 298-306.doi: 10.13560/j.cnki.biotech.bull.1985.2022-1093
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DU Dong-dong(
), QIAN Jing, LI Si-qi, LIU Wen-fei, WEI Xiang-li, LIU Chang-yong, LUO Rui-feng, KANG Li-chao()
Received:2022-09-05
Online:2023-07-26
Published:2023-08-17
Contact:
KANG Li-chao
E-mail:1642237366 @qq.com;83511807@qq.com
DU Dong-dong, QIAN Jing, LI Si-qi, LIU Wen-fei, WEI Xiang-li, LIU Chang-yong, LUO Rui-feng, KANG Li-chao. Whole Genome Sequencing and Analysis of Listeria monocytogenes Strain LMXJ15[J]. Biotechnology Bulletin, 2023, 39(7): 298-306.
| Contig | 起始Start | 终止End | 重复数Repeat count | 重复长度Repeat length/bp | 间隔长度Spacer length/bp |
|---|---|---|---|---|---|
| NODE_2 | 270451 | 270928 | 8 | 29 | 35 |
Table 1 CRISPR sequences of strain LMXJ15
| Contig | 起始Start | 终止End | 重复数Repeat count | 重复长度Repeat length/bp | 间隔长度Spacer length/bp |
|---|---|---|---|---|---|
| NODE_2 | 270451 | 270928 | 8 | 29 | 35 |
Fig. 1 SeqLogo plot of CRISPR repeats
Fig. 2 GO functional classification of genes of strain LMXJ15 The horizontal axis is the secondary classification of GO, and the vertical axis is the number of genes in the classification（right）and its percentage of the total number of genes annotated（left）. Different colors represent different Ortholog
| 功能Function | 基因Genes |
|---|---|
| Regulation of virulence | prfA、virR、agrA、lisR、perR、gmar、Rli55、Rli60、lhrC、secA2 |
| Tolerance to environmental stress | sigB、gadA、gadB、gadC、bsh、clpB、clpc、clpE、clpp、hfq、degU、mogR、Rsbv、biLE、btlB、htrA、ctsR、perR、secA2、tcsA、tig |
| Escape host immunity | sod、pgdA、oatA、orfX、orfZ、hly、plcA、plcB、mpl、svpA、sipZ、lsp、actA、srtB、inlK、inlC、lntA、sipX、 |
| Invasion/adherence | ami、iap、srtA、lap、fbpA、ctaP、lapB、recA、auto、lgt、gtca、mprF、flaA、flaC、flaE、inlA、inlB、inlF、inlH、inlJ、lpeA、dltA、codY、lmo0514、lmo0610、murA |
| Survival/proliferation | hpt、pycA、fri、relA、oppA、lplA1、prsA2、chiA、dal、fur、pgl、stp |
| Other related virulence genes | lipA、lmo2085 |
Table 2 Pathogenicity-related genes on the genome of strain LMXJ15
| 功能Function | 基因Genes |
|---|---|
| Regulation of virulence | prfA、virR、agrA、lisR、perR、gmar、Rli55、Rli60、lhrC、secA2 |
| Tolerance to environmental stress | sigB、gadA、gadB、gadC、bsh、clpB、clpc、clpE、clpp、hfq、degU、mogR、Rsbv、biLE、btlB、htrA、ctsR、perR、secA2、tcsA、tig |
| Escape host immunity | sod、pgdA、oatA、orfX、orfZ、hly、plcA、plcB、mpl、svpA、sipZ、lsp、actA、srtB、inlK、inlC、lntA、sipX、 |
| Invasion/adherence | ami、iap、srtA、lap、fbpA、ctaP、lapB、recA、auto、lgt、gtca、mprF、flaA、flaC、flaE、inlA、inlB、inlF、inlH、inlJ、lpeA、dltA、codY、lmo0514、lmo0610、murA |
| Survival/proliferation | hpt、pycA、fri、relA、oppA、lplA1、prsA2、chiA、dal、fur、pgl、stp |
| Other related virulence genes | lipA、lmo2085 |
| 菌株 Strain | abcZ | bglA | cat | dapE | dat | ldh | lhkA | ST | CC | 谱系Lineage | 登录号Accession number | 来源 Source | 年份 Year | 国家 Nation |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| LL195 | 3 | 1 | 1 | 1 | 3 | 1 | 3 | 1 | CC1 | I | HF558398.1 | 李斯特菌病人 | 1983 to 1987 | 瑞士 |
| F2365 | 3 | 1 | 1 | 1 | 3 | 1 | 3 | 1 | CC1 | I | AE017262.2 | 奶酪分离物 | 1985 | 美国 |
| NTSN | 3 | 1 | 1 | 1 | 3 | 1 | 3 | 1 | CC1 | I | CP009897.1 | 绵羊 | 2011 | 中国江苏 |
| VIMHA007 | 1 | 1 | 11 | 11 | 2 | 1 | 5 | 2 | CC2 | I | CP018149.1 | 流产的胎儿 | 2005 | 俄罗斯 |
| WSLC 1033 | 1 | 1 | 11 | 11 | 2 | 1 | 5 | 2 | CC2 | I | CP013288.1 | 绵羊 | 1961 | 美国 |
| PIR00544 | 1 | 1 | 11 | 11 | 2 | 1 | 5 | 2 | CC2 | I | CP025565.1 | NA | NA | NA |
| N2306 | 1 | 2 | 12 | 3 | 2 | 5 | 3 | 4 | CC4 | I | CP011004.1 | 李斯特菌病人血液 | 2014 | 瑞士 |
| 07PF0776 | 1 | 2 | 12 | 3 | 2 | 5 | 3 | 4 | CC4 | I | CP003414.1 | 心肌脓肿病人 | NA | 美国 |
| L312 | 1 | 2 | 12 | 3 | 2 | 5 | 3 | 4 | CC4 | I | FR733642.2 | 奶酪 | NA | NA |
| L2624 | 2 | 1 | 11 | 3 | 3 | 1 | 7 | 5 | CC5 | I | CP007686.1 | 李斯特菌人 | 2011 | 美国 |
| J2-064 | 2 | 1 | 11 | 3 | 3 | 1 | 7 | 5 | CC5 | I | CP006592.1 | 动物 | 1989 | 美国 |
| CFSAN008100 | 2 | 1 | 11 | 3 | 3 | 1 | 7 | 5 | CC5 | I | CP011398.2 | 番椒 | NA | 美国 |
| CFSAN010068 | 2 | 1 | 11 | 3 | 3 | 1 | 7 | 5 | CC5 | I | CP014250.2 | 奶酪 | 2014 | 美国 |
| CFSAN023459 | 2 | 1 | 11 | 3 | 3 | 1 | 7 | 5 | CC5 | I | CP014252.2 | 油桃 | 2014 | 美国 |
| N16-0044 | 3 | 9 | 9 | 3 | 3 | 1 | 5 | 6 | CC6 | I | CP035187.1 | 李斯特菌病人血液 | 2016 | 瑞士 |
| J1776 | 3 | 9 | 9 | 3 | 3 | 1 | 5 | 6 | CC6 | I | CP006598.1 | 李斯特菌病人 | 2002 | 美国 |
| IZSAM_Lm_hs2008 | 3 | 9 | 9 | 3 | 3 | 1 | 5 | 6 | CC6 | I | CP010346.1 | 脑脊髓液 | 2008 | 意大利 |
| J1816 | 3 | 9 | 9 | 3 | 3 | 1 | 5 | 6 | CC6 | I | CP006047.2 | 食品加工厂 | 2002 | 美国 |
| J1926 | 3 | 9 | 9 | 3 | 3 | 1 | 5 | 6 | CC6 | I | CP006600.1 | 食物 | 2002 | 美国 |
| J1817 | 3 | 9 | 9 | 3 | 3 | 1 | 5 | 6 | CC6 | I | CP006599.1 | 加工环境 | 2002 | 美国 |
| 10-092876-0769 LM12 | 3 | 9 | 9 | 3 | 3 | 1 | 5 | 6 | CC6 | I | CP019625.1 | 加工环境 | 2002 | 加拿大 |
| CFSAN006122 | 3 | 9 | 9 | 3 | 3 | 1 | 5 | 6 | CC6 | I | CP007600.2 | 奶酪 | 2013 | 美国 |
| 2015TE17781-3 | 5 | 8 | 5 | 7 | 6 | 2 | 1 | 7 | CC7 | I | CP028413.1 | 李斯特菌病人血液 | 2015 | 意大利 |
| 2015TE17781-5 | 5 | 8 | 5 | 7 | 6 | 2 | 1 | 7 | CC7 | I | CP028412.1 | 李斯特菌病人血液 | 2015 | 意大利 |
| 2015TE24968 | 5 | 8 | 5 | 7 | 6 | 2 | 1 | 7 | CC7 | I | CP014790.1 | 李斯特菌病人血液 | 2015 | 意大利 |
| 2016TE3773-1-1 | 5 | 8 | 5 | 7 | 6 | 2 | 1 | 7 | CC7 | I | CP028396.1 | 李斯特菌病人脑脊液 | 2015 | 意大利 |
| 2018TE5305-1-4 | 5 | 8 | 5 | 7 | 6 | 2 | 1 | 7 | CC7 | I | CP029372.1 | 李斯特菌病人血液 | 2018 | 意大利 |
| L1846 | 5 | 8 | 5 | 7 | 6 | 2 | 1 | 7 | CC7 | II | CP007688.1 | 李斯特菌病人 | 2009 | 美国 |
| L2676 | 5 | 8 | 5 | 7 | 6 | 2 | 1 | 7 | CC7 | II | CP007685.1 | 李斯特菌病人 | 2011 | 美国 |
| IZSAM_Lm_15_17439_A144 | 5 | 6 | 2 | 9 | 5 | 3 | 1 | 8 | CC8 | II | CP013919.1 | 李斯特菌病人血液 | 2008 | 意大利 |
| Lm N1546 | 5 | 6 | 2 | 9 | 5 | 3 | 1 | 8 | CC8 | II | CP013724.1 | 李斯特菌病人血液 | 2011 | 瑞士 |
| LMXJ15 | 5 | 6 | 2 | 9 | 5 | 3 | 1 | 8 | CC8 | II | 调理肉制品 | 2014 | 中国 新疆 | |
| R479a | 5 | 6 | 2 | 9 | 5 | 3 | 1 | 8 | CC8 | II | HG813247.1 | 熏鲑鱼 | NA | NA |
| AL4E | 6 | 5 | 6 | 4 | 1 | 4 | 1 | 9 | CC9 | II | CP023754.1 | 设备 | 1998 | 芬兰 |
| MF4545 | 6 | 5 | 6 | 4 | 1 | 4 | 1 | 9 | CC9 | II | CP025443.1 | 肉类加工设施 | 2012 | 挪威 |
| MF4626 | 6 | 5 | 6 | 4 | 1 | 4 | 1 | 9 | CC9 | II | CP025082.1 | 肉类加工设施 | 2012 | 挪威 |
| MF4697 | 6 | 5 | 6 | 4 | 1 | 4 | 1 | 9 | CC9 | II | CP025438.1 | 肉类加工设施 | 2012 | 挪威 |
| MF6172 | 6 | 5 | 6 | 4 | 1 | 4 | 1 | 9 | CC9 | II | CP025440.1 | 肉类加工设施 | 2012 | 挪威 |
| AT3E | 6 | 5 | 6 | 4 | 1 | 4 | 1 | 9 | CC9 | II | CP023752.1 | 食品 | 1995 | 芬兰 |
| WSLC1001 | 5 | 8 | 5 | 7 | 6 | 22 | 1 | 12 | CC7 | II | CP007160.1 | NA | NA | 德国 |
| 3136 | 7 | 6 | 15 | 18 | 12 | 6 | 1 | 18 | CC18 | II | CP013723.1 | 李斯特菌病人血液 | 2005 | 瑞士 |
| 3163 | 5 | 10 | 8 | 21 | 6 | 2 | 1 | 26 | CC26 | II | CP013722.1 | 李斯特菌病人血液 | 2005 | 瑞士 |
| EGDE | 6 | 5 | 6 | 20 | 1 | 4 | 1 | 35 | CC9 | II | CP023861.1 | 兔子 | 1924 | 英国 |
| WSLC 1019 | 18 | 11 | 21 | 24 | 17 | 35 | 13 | 71 | CC131 | III | CP013286.1 | 鸡肉 | 1961 | 英国 |
| SLCC2376 | 18 | 11 | 21 | 24 | 17 | 35 | 13 | 71 | CC131 | III | FR733651.1 | 禽肉 | NA | NA |
| WSLC 1020 | 18 | 11 | 21 | 24 | 17 | 35 | 13 | 71 | CC131 | III | CP013287.1 | NA | 1931 | 美国 |
| WSLC 1018 | 3 | 1 | 1 | 1 | 3 | 11 | 3 | 73 | CC1 | I | CP013285.1 | 鸡肉 | 1961 | 英国 |
| 10403S | 5 | 8 | 5 | 7 | 6 | 38 | 1 | 85 | CC7 | II | CP002002.1 | NA | NA | NA |
| ICDC-LM188 | 12 | 1 | 4 | 14 | 3 | 39 | 4 | 87 | CC87 | I | CP015593.1 | 李斯特菌病人血液 | 2008 | 中国 北京 |
| SLCC7179 | 7 | 6 | 15 | 6 | 5 | 2 | 1 | 91 | CC14 | II | FR733650.1 | 奶酪 | 1986 | 澳大利亚 |
| 08-5923 | 5 | 6 | 2 | 29 | 5 | 3 | 1 | 120 | CC8 | II | CP001604.1 | 李斯特菌病人 | 2008 | 加拿大 |
| LM6179 | 7 | 6 | 8 | 8 | 6 | 37 | 1 | 121 | CC121 | II | HG813249.1 | NA | NA | NA |
| FORC_057 | 7 | 10 | 16 | 7 | 5 | 2 | 1 | 155 | CC155 | II | CP021174.1 | 金枪鱼泡菜紫菜包饭 | 2012 | 韩国 |
| Finland 1988 | 7 | 10 | 16 | 7 | 5 | 2 | 1 | 155 | CC155 | II | CP002004.1 | NA | 1998 | 芬兰 |
| M7 | 19 | 17 | 22 | 25 | 15 | 79 | 12 | 201 | CC69 | III | CP002816.1 | 牛奶 | NA | 中国浙江 |
| HCC23 | 19 | 17 | 22 | 25 | 15 | 79 | 12 | 201 | CC69 | III | CP001175.1 | 斑点叉尾鮰 | NA | NA |
| L99 | 19 | 17 | 22 | 25 | 15 | 79 | 12 | 201 | CC69 | III | FM211688.1 | 奶酪 | 1950 | 荷兰 |
| 10-092876-1063 LM3 | 1 | 9 | 1 | 3 | 7 | 5 | 3 | 217 | CC217 | I | CP019616.1 | 小菠菜 | 2009 | 加拿大 |
| NCCP14714 | 11 | 3 | 15 | 38 | 3 | 94 | 2 | 224 | CC224 | I | CP031141.1 | 李斯特菌病人血液 | 2017 | 韩国 |
| FDAARGOS_57 | 34 | 39 | 11 | 15 | 3 | 1 | 2 | 288 | CC288 | I | CP030101.1 | 水 | 2002 | 美国 |
| WSLC1047 | 1 | 1 | 11 | 11 | 2 | 11 | 5 | 290 | CC2 | I | CP013289.1 | NA | NA | NA |
| 08-5578 | 57 | 6 | 2 | 29 | 5 | 3 | 1 | 292 | CC8 | II | CP001602.2 | 李斯特菌病人 | 2008 | 加拿大 |
| 10-092876-1763LM10 | 5 | 6 | 8 | 62 | 6 | 7 | 34 | 321 | CC321 | II | CP019623.1 | 鸡胸肉 | 2007 | 加拿大 |
| L2074 | 7 | 49 | 19 | 6 | 1 | 24 | 1 | 378 | CC19 | II | CP007689.1 | 李斯特菌病人 | 2010 | 美国 |
| J2-031 | 5 | 5 | 17 | 21 | 39 | 2 | 6 | 394 | CC415 | II | CP006593.1 | 李斯特菌病人 | 1996 | 美国 |
| N12-0935 | 7 | 7 | 10 | 4 | 5 | 24 | 1 | 403 | CC403 | II | CP038642.1 | 李斯特菌病人血液 | 2012 | 瑞士 |
| NH1 | 6 | 5 | 6 | 4 | 1 | 183 | 1 | 477 | CC9 | II | CP021325.1 | 速冻食品 | 2007 | 中国 |
| H34 | 11 | 9 | 12 | 3 | 3 | 1 | 4 | 489 | CC489 | I | CP020774.1 | 脓毒血症新生儿肺脏和血液 | 2016 | 乌拉圭 |
| Lm60 | 5 | 6 | 88 | 29 | 5 | 3 | 1 | 551 | CC8 | II | CP009258.1 | 李斯特菌病人血液 | 2006 | 瑞士 |
| L2626 | 5 | 8 | 5 | 7 | 6 | 2 | 71 | 561 | CC7 | II | CP007684.1 | 李斯特菌病人 | 2011 | 美国 |
| xysn | 7 | 88 | 106 | 120 | 1 | 249 | 1 | 626 | CC33 | II | CP007583.1 | 山羊 | 2011 | 中国 |
| F4244 | 3 | 9 | 9 | 3 | 3 | 28 | 5 | 1347 | CC6 | I | CP015508.1 | 脑脊液 | 1991 | 美国 |
Table 3 MSLT typing results of 72 strains of Listeria monocytogenes
| 菌株 Strain | abcZ | bglA | cat | dapE | dat | ldh | lhkA | ST | CC | 谱系Lineage | 登录号Accession number | 来源 Source | 年份 Year | 国家 Nation |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| LL195 | 3 | 1 | 1 | 1 | 3 | 1 | 3 | 1 | CC1 | I | HF558398.1 | 李斯特菌病人 | 1983 to 1987 | 瑞士 |
| F2365 | 3 | 1 | 1 | 1 | 3 | 1 | 3 | 1 | CC1 | I | AE017262.2 | 奶酪分离物 | 1985 | 美国 |
| NTSN | 3 | 1 | 1 | 1 | 3 | 1 | 3 | 1 | CC1 | I | CP009897.1 | 绵羊 | 2011 | 中国江苏 |
| VIMHA007 | 1 | 1 | 11 | 11 | 2 | 1 | 5 | 2 | CC2 | I | CP018149.1 | 流产的胎儿 | 2005 | 俄罗斯 |
| WSLC 1033 | 1 | 1 | 11 | 11 | 2 | 1 | 5 | 2 | CC2 | I | CP013288.1 | 绵羊 | 1961 | 美国 |
| PIR00544 | 1 | 1 | 11 | 11 | 2 | 1 | 5 | 2 | CC2 | I | CP025565.1 | NA | NA | NA |
| N2306 | 1 | 2 | 12 | 3 | 2 | 5 | 3 | 4 | CC4 | I | CP011004.1 | 李斯特菌病人血液 | 2014 | 瑞士 |
| 07PF0776 | 1 | 2 | 12 | 3 | 2 | 5 | 3 | 4 | CC4 | I | CP003414.1 | 心肌脓肿病人 | NA | 美国 |
| L312 | 1 | 2 | 12 | 3 | 2 | 5 | 3 | 4 | CC4 | I | FR733642.2 | 奶酪 | NA | NA |
| L2624 | 2 | 1 | 11 | 3 | 3 | 1 | 7 | 5 | CC5 | I | CP007686.1 | 李斯特菌人 | 2011 | 美国 |
| J2-064 | 2 | 1 | 11 | 3 | 3 | 1 | 7 | 5 | CC5 | I | CP006592.1 | 动物 | 1989 | 美国 |
| CFSAN008100 | 2 | 1 | 11 | 3 | 3 | 1 | 7 | 5 | CC5 | I | CP011398.2 | 番椒 | NA | 美国 |
| CFSAN010068 | 2 | 1 | 11 | 3 | 3 | 1 | 7 | 5 | CC5 | I | CP014250.2 | 奶酪 | 2014 | 美国 |
| CFSAN023459 | 2 | 1 | 11 | 3 | 3 | 1 | 7 | 5 | CC5 | I | CP014252.2 | 油桃 | 2014 | 美国 |
| N16-0044 | 3 | 9 | 9 | 3 | 3 | 1 | 5 | 6 | CC6 | I | CP035187.1 | 李斯特菌病人血液 | 2016 | 瑞士 |
| J1776 | 3 | 9 | 9 | 3 | 3 | 1 | 5 | 6 | CC6 | I | CP006598.1 | 李斯特菌病人 | 2002 | 美国 |
| IZSAM_Lm_hs2008 | 3 | 9 | 9 | 3 | 3 | 1 | 5 | 6 | CC6 | I | CP010346.1 | 脑脊髓液 | 2008 | 意大利 |
| J1816 | 3 | 9 | 9 | 3 | 3 | 1 | 5 | 6 | CC6 | I | CP006047.2 | 食品加工厂 | 2002 | 美国 |
| J1926 | 3 | 9 | 9 | 3 | 3 | 1 | 5 | 6 | CC6 | I | CP006600.1 | 食物 | 2002 | 美国 |
| J1817 | 3 | 9 | 9 | 3 | 3 | 1 | 5 | 6 | CC6 | I | CP006599.1 | 加工环境 | 2002 | 美国 |
| 10-092876-0769 LM12 | 3 | 9 | 9 | 3 | 3 | 1 | 5 | 6 | CC6 | I | CP019625.1 | 加工环境 | 2002 | 加拿大 |
| CFSAN006122 | 3 | 9 | 9 | 3 | 3 | 1 | 5 | 6 | CC6 | I | CP007600.2 | 奶酪 | 2013 | 美国 |
| 2015TE17781-3 | 5 | 8 | 5 | 7 | 6 | 2 | 1 | 7 | CC7 | I | CP028413.1 | 李斯特菌病人血液 | 2015 | 意大利 |
| 2015TE17781-5 | 5 | 8 | 5 | 7 | 6 | 2 | 1 | 7 | CC7 | I | CP028412.1 | 李斯特菌病人血液 | 2015 | 意大利 |
| 2015TE24968 | 5 | 8 | 5 | 7 | 6 | 2 | 1 | 7 | CC7 | I | CP014790.1 | 李斯特菌病人血液 | 2015 | 意大利 |
| 2016TE3773-1-1 | 5 | 8 | 5 | 7 | 6 | 2 | 1 | 7 | CC7 | I | CP028396.1 | 李斯特菌病人脑脊液 | 2015 | 意大利 |
| 2018TE5305-1-4 | 5 | 8 | 5 | 7 | 6 | 2 | 1 | 7 | CC7 | I | CP029372.1 | 李斯特菌病人血液 | 2018 | 意大利 |
| L1846 | 5 | 8 | 5 | 7 | 6 | 2 | 1 | 7 | CC7 | II | CP007688.1 | 李斯特菌病人 | 2009 | 美国 |
| L2676 | 5 | 8 | 5 | 7 | 6 | 2 | 1 | 7 | CC7 | II | CP007685.1 | 李斯特菌病人 | 2011 | 美国 |
| IZSAM_Lm_15_17439_A144 | 5 | 6 | 2 | 9 | 5 | 3 | 1 | 8 | CC8 | II | CP013919.1 | 李斯特菌病人血液 | 2008 | 意大利 |
| Lm N1546 | 5 | 6 | 2 | 9 | 5 | 3 | 1 | 8 | CC8 | II | CP013724.1 | 李斯特菌病人血液 | 2011 | 瑞士 |
| LMXJ15 | 5 | 6 | 2 | 9 | 5 | 3 | 1 | 8 | CC8 | II | 调理肉制品 | 2014 | 中国 新疆 | |
| R479a | 5 | 6 | 2 | 9 | 5 | 3 | 1 | 8 | CC8 | II | HG813247.1 | 熏鲑鱼 | NA | NA |
| AL4E | 6 | 5 | 6 | 4 | 1 | 4 | 1 | 9 | CC9 | II | CP023754.1 | 设备 | 1998 | 芬兰 |
| MF4545 | 6 | 5 | 6 | 4 | 1 | 4 | 1 | 9 | CC9 | II | CP025443.1 | 肉类加工设施 | 2012 | 挪威 |
| MF4626 | 6 | 5 | 6 | 4 | 1 | 4 | 1 | 9 | CC9 | II | CP025082.1 | 肉类加工设施 | 2012 | 挪威 |
| MF4697 | 6 | 5 | 6 | 4 | 1 | 4 | 1 | 9 | CC9 | II | CP025438.1 | 肉类加工设施 | 2012 | 挪威 |
| MF6172 | 6 | 5 | 6 | 4 | 1 | 4 | 1 | 9 | CC9 | II | CP025440.1 | 肉类加工设施 | 2012 | 挪威 |
| AT3E | 6 | 5 | 6 | 4 | 1 | 4 | 1 | 9 | CC9 | II | CP023752.1 | 食品 | 1995 | 芬兰 |
| WSLC1001 | 5 | 8 | 5 | 7 | 6 | 22 | 1 | 12 | CC7 | II | CP007160.1 | NA | NA | 德国 |
| 3136 | 7 | 6 | 15 | 18 | 12 | 6 | 1 | 18 | CC18 | II | CP013723.1 | 李斯特菌病人血液 | 2005 | 瑞士 |
| 3163 | 5 | 10 | 8 | 21 | 6 | 2 | 1 | 26 | CC26 | II | CP013722.1 | 李斯特菌病人血液 | 2005 | 瑞士 |
| EGDE | 6 | 5 | 6 | 20 | 1 | 4 | 1 | 35 | CC9 | II | CP023861.1 | 兔子 | 1924 | 英国 |
| WSLC 1019 | 18 | 11 | 21 | 24 | 17 | 35 | 13 | 71 | CC131 | III | CP013286.1 | 鸡肉 | 1961 | 英国 |
| SLCC2376 | 18 | 11 | 21 | 24 | 17 | 35 | 13 | 71 | CC131 | III | FR733651.1 | 禽肉 | NA | NA |
| WSLC 1020 | 18 | 11 | 21 | 24 | 17 | 35 | 13 | 71 | CC131 | III | CP013287.1 | NA | 1931 | 美国 |
| WSLC 1018 | 3 | 1 | 1 | 1 | 3 | 11 | 3 | 73 | CC1 | I | CP013285.1 | 鸡肉 | 1961 | 英国 |
| 10403S | 5 | 8 | 5 | 7 | 6 | 38 | 1 | 85 | CC7 | II | CP002002.1 | NA | NA | NA |
| ICDC-LM188 | 12 | 1 | 4 | 14 | 3 | 39 | 4 | 87 | CC87 | I | CP015593.1 | 李斯特菌病人血液 | 2008 | 中国 北京 |
| SLCC7179 | 7 | 6 | 15 | 6 | 5 | 2 | 1 | 91 | CC14 | II | FR733650.1 | 奶酪 | 1986 | 澳大利亚 |
| 08-5923 | 5 | 6 | 2 | 29 | 5 | 3 | 1 | 120 | CC8 | II | CP001604.1 | 李斯特菌病人 | 2008 | 加拿大 |
| LM6179 | 7 | 6 | 8 | 8 | 6 | 37 | 1 | 121 | CC121 | II | HG813249.1 | NA | NA | NA |
| FORC_057 | 7 | 10 | 16 | 7 | 5 | 2 | 1 | 155 | CC155 | II | CP021174.1 | 金枪鱼泡菜紫菜包饭 | 2012 | 韩国 |
| Finland 1988 | 7 | 10 | 16 | 7 | 5 | 2 | 1 | 155 | CC155 | II | CP002004.1 | NA | 1998 | 芬兰 |
| M7 | 19 | 17 | 22 | 25 | 15 | 79 | 12 | 201 | CC69 | III | CP002816.1 | 牛奶 | NA | 中国浙江 |
| HCC23 | 19 | 17 | 22 | 25 | 15 | 79 | 12 | 201 | CC69 | III | CP001175.1 | 斑点叉尾鮰 | NA | NA |
| L99 | 19 | 17 | 22 | 25 | 15 | 79 | 12 | 201 | CC69 | III | FM211688.1 | 奶酪 | 1950 | 荷兰 |
| 10-092876-1063 LM3 | 1 | 9 | 1 | 3 | 7 | 5 | 3 | 217 | CC217 | I | CP019616.1 | 小菠菜 | 2009 | 加拿大 |
| NCCP14714 | 11 | 3 | 15 | 38 | 3 | 94 | 2 | 224 | CC224 | I | CP031141.1 | 李斯特菌病人血液 | 2017 | 韩国 |
| FDAARGOS_57 | 34 | 39 | 11 | 15 | 3 | 1 | 2 | 288 | CC288 | I | CP030101.1 | 水 | 2002 | 美国 |
| WSLC1047 | 1 | 1 | 11 | 11 | 2 | 11 | 5 | 290 | CC2 | I | CP013289.1 | NA | NA | NA |
| 08-5578 | 57 | 6 | 2 | 29 | 5 | 3 | 1 | 292 | CC8 | II | CP001602.2 | 李斯特菌病人 | 2008 | 加拿大 |
| 10-092876-1763LM10 | 5 | 6 | 8 | 62 | 6 | 7 | 34 | 321 | CC321 | II | CP019623.1 | 鸡胸肉 | 2007 | 加拿大 |
| L2074 | 7 | 49 | 19 | 6 | 1 | 24 | 1 | 378 | CC19 | II | CP007689.1 | 李斯特菌病人 | 2010 | 美国 |
| J2-031 | 5 | 5 | 17 | 21 | 39 | 2 | 6 | 394 | CC415 | II | CP006593.1 | 李斯特菌病人 | 1996 | 美国 |
| N12-0935 | 7 | 7 | 10 | 4 | 5 | 24 | 1 | 403 | CC403 | II | CP038642.1 | 李斯特菌病人血液 | 2012 | 瑞士 |
| NH1 | 6 | 5 | 6 | 4 | 1 | 183 | 1 | 477 | CC9 | II | CP021325.1 | 速冻食品 | 2007 | 中国 |
| H34 | 11 | 9 | 12 | 3 | 3 | 1 | 4 | 489 | CC489 | I | CP020774.1 | 脓毒血症新生儿肺脏和血液 | 2016 | 乌拉圭 |
| Lm60 | 5 | 6 | 88 | 29 | 5 | 3 | 1 | 551 | CC8 | II | CP009258.1 | 李斯特菌病人血液 | 2006 | 瑞士 |
| L2626 | 5 | 8 | 5 | 7 | 6 | 2 | 71 | 561 | CC7 | II | CP007684.1 | 李斯特菌病人 | 2011 | 美国 |
| xysn | 7 | 88 | 106 | 120 | 1 | 249 | 1 | 626 | CC33 | II | CP007583.1 | 山羊 | 2011 | 中国 |
| F4244 | 3 | 9 | 9 | 3 | 3 | 28 | 5 | 1347 | CC6 | I | CP015508.1 | 脑脊液 | 1991 | 美国 |
Fig. 3 Phylogenetic tree of 72 Listeria monocytogenes strains based on the housekeeping gene sequences of MLST
Fig. 4 Whole genome alignment analysis
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