Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (8): 220-233.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0115

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Characteristics Analysis of Seed Microrhizome Gene Expression of Polygonatum cyrtonema

LIU Bao-cai1,2(), CHEN Jing-ying1,2(), ZHANG Wu-jun1,2, HUANG Ying-zhen1,2, ZHAO Yun-qing1,2, LIU Jian-chao3, WEI Zhi-cheng4   

  1. 1. Institute of Agricultural Bioresource, Fujian Academy of Agricultural Sciences, Fuzhou 350003
    2. Research Center for Medicinal Plant, Fujian Academy of Agricultural Sciences, Fuzhou 350003
    3. Agricultural and Rural Bureau of Shaowu City, Nanping City, Fujian Province, Shaowu 354000
    4. Fujian Heping Ancient Town Agricultural Development Co., Ltd., Shaowu 354000
  • Received:2023-02-13 Online:2023-08-26 Published:2023-09-05
  • Contact: CHEN Jing-ying E-mail:626813844@qq.com;cjy6601@163.com

Abstract:

The germination process of Polygonatum cyrtonema seeds have a special germination phenomenon of micro rhizomes formation. The purpose of explaining the changes of key genes in the germination process is to lay a foundation for related research on seed physiology, morphological structure development and so on. In this paper, based on high-throughput sequencing technology, the transcriptome sequencing and bioinformatics analysis were performed on the seeds of P. cyrtonema in different germination states. The results showed that 17 907 differentially expressed genes were identified in microrhizome formation, compared to radicle breakthrough seed coat, these genes were in significantly different expressions in terms of metabolic process, catalytic activity, protein phosphorylation, etc. Pathway significance enrichment indicated that the differential genes were mainly enriched in plant hormone signal transduction, starch and sucrose metabolism, flavonoid biosynthesis, and other pathways. Genes enriched were abundantly expressed in plant hormone signal transduction pathways. Especially, the expression genes were up-regulated in brassinosteroid biosynthesis pathway. There were 26 833 significant differences Unigenes before and after the microrhizomes turned green, mainly enriched in metabolic process, peptide biosynthesis process, catalytic activity and other pathways. The differential genes were mainly enriched in, starch and sucrose metabolism, photosynthesis and other pathways by KEGG pathway enrichment analysis. The germination process of P. cyrtonema seeds is a complex regulatory network of a series of genes, and genes related with in brassinosteroid biosynthesis pathway may have an important role in the formation of microhizome. The results also provides a reference for in-depth research on the micro-rhizome formation physiology, micro-rhizome rapid expansion in production, and microhizom exploitation.

Key words: Polygonatum cyrtonema, seeds, microrhizome, plant hormone, starch and sucrose, photosynthesis, brassinosteroid