Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (9): 105-116.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0091

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Molecular Mechanism of Transcriptional Factor AtbHLH68 in Regulating Cell Wall Development by Transcriptome Analysis

LIN Hong-yan1,2,3(), GUO Xiao-rui1,2,3, LIU Di1,2,3, LI Hui1,2,3, LU Hai1,2,3()   

  1. 1. National Engineering Research Center of Tree Breeding and Ecological Retoration, Beijing 100083
    2. The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, Beijing 100083
    3. School of Biological Science and Technology, Beijing Forestry University, Beijing 100083
  • Received:2023-02-07 Online:2023-09-26 Published:2023-10-24
  • Contact: LU Hai E-mail:2638859438@qq.com;luhai1974@bjfu.edu.cn

Abstract:

Cell wall is a plant-specific structure and plays important role in morphogenesis, water and nutrients transportation, and biotic and abiotic resistance. Previous studies have shown that AtbHLH68 as a member of the 10th bHLH protein subfamily, is expressed in the vascular tissue of Arabidopsis thaliana stem. To investigate its molecular mechanism of AtbHLH68 in cell wall development, the estradiol-induced pER8-AtbHLH68 expression system was established in Arabidopsis thaliana. Quantitative real-time PCR(RT-qPCR)results showed that 10 μmol/L estradiol treatment for 4 h can effectively induce the expression of AtbHLH68, and the expression of AtbHLH68 gradually increased with the prolongation of induction time, reaching 29 times after 8 h. The differentially expressed genes(DEGs)induced by AtbHLH68 in Arabidopsis stems were detected by transcriptome analysis. Compared with the control group, after 8 h of treatment with 10 μmol/L estradiol, a total of 2 334 DEGs were detected, among which 831 genes were significantly up-regulated and 1 503 genes were significantly down-regulated. The significantly enrichment GO terms were mainly related to cell wall components, defense response, pectin modification and degradation and hormone response. KEGG analysis showed that DEGs involved in the processes such as pectin modification and lignin biosynthetic metabolism. These results indicated that the DEGs involved in the above processes may be regulated by AtbHLH68 directly or indirectly. This study provided theoretical evidences to clarify the molecular mechanism of transcription factor AtbHLH68 in cell wall development.

Key words: induced expression, AtbHLH68, transcriptome, cell wall