Control of Pepper Fusarium Wilt by Bacillus subtilis Ya-1 and Its Effect on Rhizosphere Fungal Microbial Community

doi:10.13560/j.cnki.biotech.bull.1985.2022-1457

Abstract

Abstract:

This work aims to investigate the control effects of the biocontrol bacterium Bacillus subtilis Ya-1 on pepper Fusarium wilt and its effect on rhizosphere soil fungal community and diversity after pepper is infected by Fusarium oxysporum. B. subtilis Ya-1 was used as inoculum to investigate its control effect on pepper Fusarium wilt, and high-throughput sequencing was used to study the changes of rhizosphere soil fungal community by F. oxysporum infection between before inoculation and at 10 d and 20 d after inoculation. Compared with FOC treatment（C）, the control effects of Ya-1+FOC（D）at day 10 and day 20 were 77.93% and 77.26%, respectively. The fungal α diversity of B. subtilis Ya-1 and F. oxysporum capsicum groups was higher than that of F. oxysporum group at day 10 and day 20. The composition of fungal community changed significantly after treatment. At the same time, the fungal community of the treatment group and the control group were significantly different, and the fungal community of the FOC and Ya-1+FOC treatment were also significantly different（P< 0.05）. The dominant fungal populations in all treatments were Ascomycota, Zygomycota and Basidiomycota. At day 10 and day 20, the relative abundance of inoculated pathogens（OTU_1）was significantly different between FOC and Ya-1+FOC treatment. The total relative abundance of Mortierella increased and OTU_1 decreased at day 10 and day 20, respectively. Compared with the control group, the number of nodes decreased after inoculation with Fusarium wilt, while the number of positive junctions decreased after treated with B. subtilis Ya-1. B. subtilis Ya-1 treatment had a promising control effect on pepper Fusarium wilt, while it also changed the diversity and structure of rhizosphere fungal community and reduced the complexity of fungal community and abundance of pathogenic bacteria in rhizosphere soil.

Key words: Bacillus subtilis, pepper Fusarium wilt, microbial community, pepper, rhizo sphere, fungi

ZHAO Zhi-xiang, WANG Dian-dong, ZHOU Ya-lin, WANG Pei, YAN Wan-rong, YAN Bei, LUO Lu-yun, ZHANG Zhuo. Control of Pepper Fusarium Wilt by Bacillus subtilis Ya-1 and Its Effect on Rhizosphere Fungal Microbial Community[J]. Biotechnology Bulletin, 2023, 39(9): 213-224.

Figures/Tables 10

References 41

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处理 Treatment	发病率Incidence rate /%		防治效果 Control effect /%
处理 Treatment	10 d	20 d	10 d	20 d
A（CK）	-	-	-	-
B（Ya-1）	-	-	-	-
C（FOC）	32.08 A	45.83 A	-	-
D（Ya-1+FOC）	7.08 B	10.42 B	77.93	77.26

处理 Treatment	发病率Incidence rate /%		防治效果 Control effect /%
处理 Treatment	10 d	20 d	10 d	20 d
A（CK）	-	-	-	-
B（Ya-1）	-	-	-	-
C（FOC）	32.08 A	45.83 A	-	-
D（Ya-1+FOC）	7.08 B	10.42 B	77.93	77.26

分组Group	Shannon index	Chao1
0 d	7.05±0.19ab	2099.63±72.01bc
10d-A	7.11±0.17ab	2381.63±114.67b
10d-B	7.47±0.13a	2244.79±70.87b
10d-C	5.36±0.27d	2078.28±120.13bc
10d-D	6.64±0.04bc	2382.08±75.46b
20d-A	6.78±0.28bc	1802.08±101.82c
20d-B	6.79±0.27bc	2081.37±72.32bc
20d-C	6.17±0.36ac	2234.21±158.02b
20d-D	6.62±0.23abc	2777.5±115.61a

分组Group	Shannon index	Chao1
0 d	7.05±0.19ab	2099.63±72.01bc
10d-A	7.11±0.17ab	2381.63±114.67b
10d-B	7.47±0.13a	2244.79±70.87b
10d-C	5.36±0.27d	2078.28±120.13bc
10d-D	6.64±0.04bc	2382.08±75.46b
20d-A	6.78±0.28bc	1802.08±101.82c
20d-B	6.79±0.27bc	2081.37±72.32bc
20d-C	6.17±0.36ac	2234.21±158.02b
20d-D	6.62±0.23abc	2777.5±115.61a

分组 Group	非参数多反应置换法MRPP		相似性分析ANOSIM		非参数多变量置换法PERMANOVA
分组 Group	δ	P	R	P	F	P
0 d vs 10 d	0.6067	0.016	0.3198	0.001	2.5237	0.004
0 d vs 20 d	0.6366	0.003	0.2697	0.031	2.3031	0.004
10 d vs 20 d	0.6228	0.085	0.0281	0.143	1.4528	0.11