Research Progress in the Regulation of Chlorogenic Acid Biosynthesis and Its Application

doi:10.13560/j.cnki.biotech.bull.1985.2023-0728

Abstract

Abstract:

Chlorogenic acid（CGA）is an important phenolic acid secondary metabolite widely found in plant kingdom. CGA plays an important role in plant growth and development, resistance to biotic and abiotic stresses. In addition, it has a multiple biological activities and pharmacological functions, and plays an important role in application potential in anti-inflammatory, antibacterial and hypoglycemic. However, the content of CGA is usually very low in plants, which seriously restricts its development and utilization values. Therefore, effectively improving CGA content in plants is particularly crucial. Recently, many researchers have made important advances on improving the CGA content in plants by genetic engineering, stress and hormone treatment. On this basis, researchers have also initiated new explorations on the biosynthesis of CGA and its molecular mechanism. This would provide new ideas for improving the CGA content in plants. In view of this, this paper reviews the research progresses on the structure and function, biosynthesis and regulation of CGA, and systematically analyzes the effects of key rate-limiting enzymes, such as PAL, C4H and 4CL, is involved in the synthesis of CGA. Moreover, we elucidate the role of transcription factors MYB, WRKY and bHLH in regulating the biosynthesis of CGA. Meanwhile, we comprehensively summarize the effects of exogenous factors, e.g., biotic/abiotic stresses, plant hormones, light quality and photoperiod on the CGA content and biosynthesis in plants，and introduce the mechanism of CGA in improving animal and human health. Finally, we explore and prospect the unsolved problems and future research directions in the CGA. It is aimed to offer some valuable references for the rational development and utilization of CGA and the improvement of crop resistance.

Key words: chlorogenic acid, biosynthesis, transcription factors, exogenous induction, regulation

CHEN Zhi-min, LI Cui, WEI Ji-tian, LI Xin-ran, LIU Yi, GUO Qiang. Research Progress in the Regulation of Chlorogenic Acid Biosynthesis and Its Application[J]. Biotechnology Bulletin, 2024, 40(1): 57-71.

Figures/Tables 3

References 113

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名称Name	药理活性Pharmacological activity	参考文献Reference
3-咖啡酰奎宁酸 3-caffeoylquinic acid	抗炎、神经保护、抗自由基、抗肿瘤、调节血糖血脂	[5⇓-7]
4-咖啡酰奎宁酸 4-caffeoylquinic acid	抗氧化、抗炎	[8-9]
5-咖啡酰奎宁酸 5-caffeoylquinic acid	抗氧化、神经保护	[10-11]
3-阿魏酰奎宁酸 3-feruloylquinic acid	抗氧化、抗炎	[12-13]
4-阿魏酰奎宁酸 4-feruloylquinic acid	抗氧化、抗炎	[12-13]
5-阿魏酰奎宁酸 5-feruloylquinic acid	抗氧化、抗炎、抗菌、抗糖尿病	[12,14]
1,3-二咖啡酰奎宁酸 1,3-dicaffeoylquinic acid	抗炎、抗癌	[15-16]
1,5-二咖啡酰奎宁酸 1,5-dicaffeoylquinic acid	抑制α-葡萄糖苷酶、神经保护	[17-18]
3,4-二咖啡酰奎宁酸 3,4-dicaffeoylquinic acid	抗炎、抗自由基、抗病毒	[6,19]
3,5-二咖啡酰奎宁酸 3,5-dicaffeoylquinic acid	抗氧化、抗病毒、抗炎	[11,19 -20]
4,5-二咖啡酰奎宁酸 4,5-dicaffeoylquinic acid	抗病毒、抗炎、抗自由基、神经保护	[5-6,19]
1,3,5-三咖啡酰奎宁酸 1,3,5-tricaffeoylquinic acid	抗氧化、神经保护	[21-22]
3,4,5-三咖啡酰奎宁酸 3,4,5-tricaffeoylquinic acid	抗病毒	[10]
绿原酸甲酯 Methyl chlorogenate	抗增殖、清除自由基、抗炎	[23-24]
绿原酸乙酯 Chlorogenic ethyl ester	抗氧化、抑制黑色素	[25-26]
绿原酸丁酯 Chlorogenic acid butyl ester	抗氧化、抑制黑色素	[26]

名称Name	药理活性Pharmacological activity	参考文献Reference
3-咖啡酰奎宁酸 3-caffeoylquinic acid	抗炎、神经保护、抗自由基、抗肿瘤、调节血糖血脂	[5⇓-7]
4-咖啡酰奎宁酸 4-caffeoylquinic acid	抗氧化、抗炎	[8-9]
5-咖啡酰奎宁酸 5-caffeoylquinic acid	抗氧化、神经保护	[10-11]
3-阿魏酰奎宁酸 3-feruloylquinic acid	抗氧化、抗炎	[12-13]
4-阿魏酰奎宁酸 4-feruloylquinic acid	抗氧化、抗炎	[12-13]
5-阿魏酰奎宁酸 5-feruloylquinic acid	抗氧化、抗炎、抗菌、抗糖尿病	[12,14]
1,3-二咖啡酰奎宁酸 1,3-dicaffeoylquinic acid	抗炎、抗癌	[15-16]
1,5-二咖啡酰奎宁酸 1,5-dicaffeoylquinic acid	抑制α-葡萄糖苷酶、神经保护	[17-18]
3,4-二咖啡酰奎宁酸 3,4-dicaffeoylquinic acid	抗炎、抗自由基、抗病毒	[6,19]
3,5-二咖啡酰奎宁酸 3,5-dicaffeoylquinic acid	抗氧化、抗病毒、抗炎	[11,19 -20]
4,5-二咖啡酰奎宁酸 4,5-dicaffeoylquinic acid	抗病毒、抗炎、抗自由基、神经保护	[5-6,19]
1,3,5-三咖啡酰奎宁酸 1,3,5-tricaffeoylquinic acid	抗氧化、神经保护	[21-22]
3,4,5-三咖啡酰奎宁酸 3,4,5-tricaffeoylquinic acid	抗病毒	[10]
绿原酸甲酯 Methyl chlorogenate	抗增殖、清除自由基、抗炎	[23-24]
绿原酸乙酯 Chlorogenic ethyl ester	抗氧化、抑制黑色素	[25-26]
绿原酸丁酯 Chlorogenic acid butyl ester	抗氧化、抑制黑色素	[26]