Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (6): 271-280.doi: 10.13560/j.cnki.biotech.bull.1985.2023-1146
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TIAN Sheng-ni(), ZHANG Qin, DONG Yu-fei, DING Zhou, YE Ai-hua, ZHANG Ming-zhu()
Received:
2023-12-05
Online:
2024-06-26
Published:
2024-04-28
Contact:
ZHANG Ming-zhu
E-mail:tiansn@ahau.edu.cn;2021037@ahau.edu.cn
TIAN Sheng-ni, ZHANG Qin, DONG Yu-fei, DING Zhou, YE Ai-hua, ZHANG Ming-zhu. Effects of Acid Mine Drainage on Physicochemical Factors and Nitrogen-fixing Microorganisms in the Root Zone of Mature Rice[J]. Biotechnology Bulletin, 2024, 40(6): 271-280.
供试土壤 Tested soil | TN/ (g·kg-1) | NO3-/ (mg·kg-1) | NH4+/ (mg·kg-1) | OM/ (g·kg-1) | TP/ (g·kg-1) | pH | SO42-/ (mg·kg-1) | Cu/ (mg·kg-1) | Cd/ (mg·kg-1) | Pb/ (mg·kg-1) | Zn/ (mg·kg-1) |
---|---|---|---|---|---|---|---|---|---|---|---|
污染土AMD contaminated soil | 0.97±0.10 | 6.42±0.60 | 7.52±0.90 | 13.01±1.72 | 0.72±0.07 | 4.68±0.05 | 282.41±6.29 | 151.82±5.94 | 2.49±0.11 | 159.02±5.19 | 210.30±5.39 |
未污染土Uncontaminated soil | 0.98±0.90 | 3.89±0.51 | 8.05±0.64 | 8.29±0.44 | 0.67±0.01 | 6.18±0.07 | 132.87±4.79 | 20.85±0.57 | 1.99±0.13 | 28.30±0.94 | 90.96±2.26 |
Table 1 Physical and chemical properties of soil before planting rice
供试土壤 Tested soil | TN/ (g·kg-1) | NO3-/ (mg·kg-1) | NH4+/ (mg·kg-1) | OM/ (g·kg-1) | TP/ (g·kg-1) | pH | SO42-/ (mg·kg-1) | Cu/ (mg·kg-1) | Cd/ (mg·kg-1) | Pb/ (mg·kg-1) | Zn/ (mg·kg-1) |
---|---|---|---|---|---|---|---|---|---|---|---|
污染土AMD contaminated soil | 0.97±0.10 | 6.42±0.60 | 7.52±0.90 | 13.01±1.72 | 0.72±0.07 | 4.68±0.05 | 282.41±6.29 | 151.82±5.94 | 2.49±0.11 | 159.02±5.19 | 210.30±5.39 |
未污染土Uncontaminated soil | 0.98±0.90 | 3.89±0.51 | 8.05±0.64 | 8.29±0.44 | 0.67±0.01 | 6.18±0.07 | 132.87±4.79 | 20.85±0.57 | 1.99±0.13 | 28.30±0.94 | 90.96±2.26 |
Fig. 2 Characteristics of soil physical and chemical factors under different treatments A: AMD irrigated contaminated soil; B: clean water irrigated contaminated soil; CK: clean water irrigated uncontaminated soil. Different lowercase letters indicate significant difference between different treatments (P < 0.05). The same below
Fig. 3 Differences in diversity and community structure of nitrogen-fixing microorganisms in rice root zone soil under different treatments a: Box plots of Chao1 and Shannon index diversity of azotobacter in rice root zone soil samples under different treatments; b: PcoA analysis of nitrogen-fixing microbial communities in rice root zone
Fig. 4 Differences in community composition of nitrogen-fixing microorganisms in rice root zone soil under different treatments a: Distribution of azotobacter in rice root zone soil samples under different treatments; b: LEfSe was based on community difference analysis among different treatment groups A, B and CK, and only groups with LDA values > 2 were clearly displayed
养分因子Nutrient factor | 回归方程Regression equation | R2 | P |
---|---|---|---|
TN | Y=1.778-4225.623×Sinorhizobium-47.255×Desulfovibrio+456.497×Azoarcus-97.577×Azospira+1.902×Geobacter | 1 | 0.000*** |
NO3- | Y=8.099-1661.173×Desulfurivibrio | 1 | 0.003*** |
OM | Y=14.024-2588.656×Rhodopseudomonas | 0.557 | 0.013** |
TP | Y=89.211-97715.976×Xanthobacter-25902.225×Pelomonas-693.042×Desulfovibrio+1097.825×Rubrivivax+13487.397×Desulfobulbus | 0.994 | 0.000*** |
SO42- | Y=-357.746+614469.605×Frankia+91743.681×Sideroxydans | 0.903 | 0.000*** |
Table 1 Stepwise regression analysis of nutrient factors in rice root zone soil and each azotobacter genus
养分因子Nutrient factor | 回归方程Regression equation | R2 | P |
---|---|---|---|
TN | Y=1.778-4225.623×Sinorhizobium-47.255×Desulfovibrio+456.497×Azoarcus-97.577×Azospira+1.902×Geobacter | 1 | 0.000*** |
NO3- | Y=8.099-1661.173×Desulfurivibrio | 1 | 0.003*** |
OM | Y=14.024-2588.656×Rhodopseudomonas | 0.557 | 0.013** |
TP | Y=89.211-97715.976×Xanthobacter-25902.225×Pelomonas-693.042×Desulfovibrio+1097.825×Rubrivivax+13487.397×Desulfobulbus | 0.994 | 0.000*** |
SO42- | Y=-357.746+614469.605×Frankia+91743.681×Sideroxydans | 0.903 | 0.000*** |
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