Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (8): 164-173.doi: 10.13560/j.cnki.biotech.bull.1985.2024-0237
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YU Niu(), LIU Fan, YANG Jin-chang()
Received:
2024-03-11
Online:
2024-08-26
Published:
2024-07-31
Contact:
YANG Jin-chang
E-mail:jcyang@caf.ac.cn
YU Niu, LIU Fan, YANG Jin-chang. Cloning of SgTPS7 in Sindora glabra and Its Function in Terpene Synthesis and Abiotic Stress[J]. Biotechnology Bulletin, 2024, 40(8): 164-173.
名称 Primer name | 序列 Primer sequence(5'-3') |
---|---|
SgTPS7-F | ATGACTAGATCCACAGCAGGC |
SgTPS7-R | CTATTTATTGTGATCAATTGCTATGG |
qSgTPS7-F | GCATATCTCGGTATCCGTGG |
qSgTPS7-R | GCAGATCATTCCGAGAATCC |
Action-2-F | CATGAAGTGTGATGTGGATA |
Action-2-R | CCTTGCTCATTCTATCAGCA |
Table 1 Primers’ sequences
名称 Primer name | 序列 Primer sequence(5'-3') |
---|---|
SgTPS7-F | ATGACTAGATCCACAGCAGGC |
SgTPS7-R | CTATTTATTGTGATCAATTGCTATGG |
qSgTPS7-F | GCATATCTCGGTATCCGTGG |
qSgTPS7-R | GCAGATCATTCCGAGAATCC |
Action-2-F | CATGAAGTGTGATGTGGATA |
Action-2-R | CCTTGCTCATTCTATCAGCA |
Fig. 3 Phylogenetic tree analysis of TPS homologous protein sequences in different plants PtTPS: Populus trichocarpa; CoTPS: Copaifera officinalis; AtTPS: Arabidopsis thaliana; StTPS: Solanum Tuberosum
Fig. 4 Expression and purification analysis of SgTPS7 protein A: SgTPS7 protein expression[M: protein marker; 0: control(without IPTG); 1: 15℃ for 16 h; 2: 37℃ for 16 h]. B: SgTPS7 protein solubility(1: supernatant; 2: precipitation). C: SgTPS7 protein purification(1: outflow; 2-4: 100 mmol/L imidazole elution; 5-6: 300 mmol/L imidazole elution). D: Purified SgTPS7 protein[1: BSA(2.00 μg); 2: SgTPS7 protein(2.00 μg)]
Fig. 5 GC-MS analysis of enzymatic products of SgTPS7 protein 1, 2, 3, 4 and 5 are ylangene, β-copaene, germacrene D, nerolidol, and linalool, respectively
Fig. 6 Expressions of SgTPS7 under various stresses Different lowercase letters indicate significant statistical differences of SgTPS7 expressions from the same tissue at different time points(P<0.05)
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