Research Progress in the Roles of Plant Glycine-rich Protein Family

doi:10.13560/j.cnki.biotech.bull.1985.2024-0795

Abstract

Abstract:

Glycine-rich proteins (GRPs) are a superfamily of proteins distinguished by their semi-repetitive glycine-rich sequences. Plant GRPs have many members and serve a variety of essential functions. The current researches show that plant GRPs play critical roles in plant growth and development, and respond to environmental factors. The first GRP gene was isolated and identified in plants as early as 1986. In recent years, significant advancements have been made in understanding the roles of GRPs in plant growth and development, and stress responses, thereby enriching our knowledge on their functions. However, there remains a lack of systematic reviews regarding the functions of plant GRPs in the domestic literature. Therefore, this article systematically summarizes the structural characteristics, family classification, and subcellular localization of the plant GRPs family, along with the latest research on their roles in plant growth and development, and stress regulation. Based on the variations in sequence structure, the plant GRPs were categorized into five major groups, with the Ⅳ category could further divide into four subfamilies. The expression of GRP genes shows significant tissue specificity and developmental stage specificity, also is adjusted by various environmental factors. GRPs serve not only as crucial components of cellular structure, but are also actively involved in biological processes under both normal and stressful conditions. Moreover, they may possess certain cross-organelle shuttle capabilities when faced with adverse stresses. Through the comprehensive review, the article aims to provide valuable insights and references for studies in related fields in China, thereby advancing the study of plant GRPs and offering guidance for future research directions.

Key words: glycine-rich protein (GRP), functional diversity, growth and development, stress resistance, research progress

WANG Bin, LIN Wei, XIAO Yan-hui, YUAN Xiao. Research Progress in the Roles of Plant Glycine-rich Protein Family[J]. Biotechnology Bulletin, 2025, 41(2): 1-17.

Figures/Tables 5

References 102

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植物种类 Plant species	基因名称 Gene name	亚细胞定位 Subcellular localization	功能描述 Function	参考文献 Reference
拟南芥	AtGRP2	细胞质和细胞核	盐胁迫诱导其表达，与转录后调控相关蛋白互作，参与转录后调控	［42］
	AtGPRP3	细胞核	与CAT2和CAT3在细胞核中互作，可能参与细胞ROS代谢调节	［39］
	AtGRP5	液泡	与植物细胞伸长有关	［43］
	AtGRP9	细胞质和细胞壁	被盐胁迫诱导，参与根维管组织木质素合成调控	［37］
	AtGR-RBP2	线粒体、细胞质和细胞核	受低温胁迫诱导，通过影响线粒体相关基因表达，促进低温胁迫下植物生长；可能抑制植物开花	［47-48］
	AtGR-RBP7	细胞核和细胞质	调控昼夜节律和开花时间	［49］
	AtGR-RBP8	细胞核和细胞质	RNA拼接	［50］
	AtPSS1	质膜	镰刀菌（Fusarium virguliforme）诱导其表达，具有增强镰刀菌引起的猝死综合征（sudden death syndrome）的抗性	［25］
水稻	OsGRP3	细胞核和细胞质	受干旱和ABA处理所诱导，通过调控ROS代谢酶相关基因mRNA的稳定性，正向调控水稻耐旱性	［46］
水稻	OsGR-RBP4	细胞核和细胞质	高温胁迫诱导其表达，高温处理后从细胞核转移至细胞质，能增强水稻的耐热性	［45］
番茄	SlRZ1AL	细胞核	促进果实发育和成熟，提高番茄红素含量	［41］
番茄	SlGRP1	细胞核和细胞质	具有RNA溶解能力，参与昼夜节律调节	［51］
玉米	ZmGR-RBP2，ZmGR-RBP3	叶绿体	未知	［12］
百合	LsGRP1	质膜和细胞壁	表达能被水杨酸（salicylic acid， SA）和椭圆形灰霉菌（Botrytis elliptica）诱导	［36］
黄瓜	CsGR-RBP3	线粒体	受低温诱导，能通过调控ROS代谢酶活性提高黄瓜果实耐冷性，其表达同时还受MYB62转录因子的调控	［44， 52-53］
菜薹	BcGRP23	叶绿体	多种植物激素处理可上调其表达，能与BRI1-EMS-SUPPRESSOR 1（BES1）互作，并直接激活其表达，参与生长发育调控	［54］
甘蔗	Sc-GRP	细胞质	参与细胞渗透调节	［55］
中国樱桃	PpcGRP4	细胞核	提高抗氧化活性	［56］
	PpcGRP6	线粒体	促进低温条件下的种子萌发，提高抗氧化活性
	PpcGRP7	细胞质、细胞核和线粒体	促进低温条件下的种子萌发，提高抗氧化活性
辣椒	CaGR-RBP1	细胞核	在细胞核与CaPIK1互作，抑制细胞死亡和防御反应	［57］
高粱	SbGR-RBP	细胞核和细胞质	响应热胁迫，在Ca²⁺存在下能与钙调素CaM互作	［58］

植物种类 Plant species	基因名称 Gene name	亚细胞定位 Subcellular localization	功能描述 Function	参考文献 Reference
拟南芥	AtGRP2	细胞质和细胞核	盐胁迫诱导其表达，与转录后调控相关蛋白互作，参与转录后调控	［42］
	AtGPRP3	细胞核	与CAT2和CAT3在细胞核中互作，可能参与细胞ROS代谢调节	［39］
	AtGRP5	液泡	与植物细胞伸长有关	［43］
	AtGRP9	细胞质和细胞壁	被盐胁迫诱导，参与根维管组织木质素合成调控	［37］
	AtGR-RBP2	线粒体、细胞质和细胞核	受低温胁迫诱导，通过影响线粒体相关基因表达，促进低温胁迫下植物生长；可能抑制植物开花	［47-48］
	AtGR-RBP7	细胞核和细胞质	调控昼夜节律和开花时间	［49］
	AtGR-RBP8	细胞核和细胞质	RNA拼接	［50］
	AtPSS1	质膜	镰刀菌（Fusarium virguliforme）诱导其表达，具有增强镰刀菌引起的猝死综合征（sudden death syndrome）的抗性	［25］
水稻	OsGRP3	细胞核和细胞质	受干旱和ABA处理所诱导，通过调控ROS代谢酶相关基因mRNA的稳定性，正向调控水稻耐旱性	［46］
水稻	OsGR-RBP4	细胞核和细胞质	高温胁迫诱导其表达，高温处理后从细胞核转移至细胞质，能增强水稻的耐热性	［45］
番茄	SlRZ1AL	细胞核	促进果实发育和成熟，提高番茄红素含量	［41］
番茄	SlGRP1	细胞核和细胞质	具有RNA溶解能力，参与昼夜节律调节	［51］
玉米	ZmGR-RBP2，ZmGR-RBP3	叶绿体	未知	［12］
百合	LsGRP1	质膜和细胞壁	表达能被水杨酸（salicylic acid， SA）和椭圆形灰霉菌（Botrytis elliptica）诱导	［36］
黄瓜	CsGR-RBP3	线粒体	受低温诱导，能通过调控ROS代谢酶活性提高黄瓜果实耐冷性，其表达同时还受MYB62转录因子的调控	［44， 52-53］
菜薹	BcGRP23	叶绿体	多种植物激素处理可上调其表达，能与BRI1-EMS-SUPPRESSOR 1（BES1）互作，并直接激活其表达，参与生长发育调控	［54］
甘蔗	Sc-GRP	细胞质	参与细胞渗透调节	［55］
中国樱桃	PpcGRP4	细胞核	提高抗氧化活性	［56］
	PpcGRP6	线粒体	促进低温条件下的种子萌发，提高抗氧化活性
	PpcGRP7	细胞质、细胞核和线粒体	促进低温条件下的种子萌发，提高抗氧化活性
辣椒	CaGR-RBP1	细胞核	在细胞核与CaPIK1互作，抑制细胞死亡和防御反应	［57］
高粱	SbGR-RBP	细胞核和细胞质	响应热胁迫，在Ca²⁺存在下能与钙调素CaM互作	［58］

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