Roles of TIFY Transcription Factors in Plant Growth, Development and Stress Adaptation

doi:10.13560/j.cnki.biotech.bull.1985.2025-0914

Abstract

Abstract:

TIFY transcription factors are a class of regulators unique to plants, which harbor a highly conserved TIF[F/Y]XG motif and can be divided into four sub-families: TIFY, JAZ, PPD and ZML. Driven by advances in plant genomics and functional genomics, TIFY transcription factor genes have been systematically identified in a wide range of plant species, and the size of this gene family varies markedly among different plants. Here we summarize the structural features of TIFY transcription factors and systematically delineate their roles in growth and development (e.g., leaf morphogenesis, floral organ, etc.), hormonal signal transduction (especially jasmonic acid, ABA, etc.), and responses to diverse environmental stresses, including biotic attack as well as cold, drought. Although TIFY proteins have been conclusively implicated in plant growth, development, and stress responses, their precise mechanistic actions remain elusive, and the fine-tuning of their functions by post-translational modifications (e.g., methylation, phosphorylation, and ubiquitination) awaits further dissection. In the future, comparative genomics can be integrated to systematically analyze the collinear blocks and evolutionary trajectories of TIFY genes across different families, genera, and ploidy levels, thereby uncovering their functional differentiation rules. Leveraging structural biology techniques such as X-ray crystallography and cryo-electron microscopy, the three-dimensional structures of TIFY core domains (e.g., TIFY and JAS) can be resolved to pinpoint their interaction interfaces with target proteins and hormonal ligands. Coupled with CRISPR-Cas9-mediated gene editing, this will enable in-depth dissection of TIFY regulatory mechanisms and facilitate directed genetic improvement via genetic-engineering strategies, ultimately providing important candidate genes for breeding next-generation crops with robust stress tolerance and superior high-yield traits.

Key words: TIFY, gene family, transcription factor, growth and development, hormone signal, JA, stress response, three-dimensional structure

SONG Wen-qing, CHEN Zhi-xiang, LI Yu-qian, WANG Hai-long, XING Guo-fang, ZHANG Jie-wei. Roles of TIFY Transcription Factors in Plant Growth, Development and Stress Adaptation[J]. Biotechnology Bulletin, 2026, 42(9): 70-81.

Figures/Tables 3

References 100

[1]	Todeschini AL, Georges A, Veitia RA. Transcription factors: specific DNA binding and specific gene regulation [J]. Trends Genet, 2014, 30(6): 211-219.
[2]	Vanholme B, Grunewald W, Bateman A, et al. The tify family previously known as ZIM [J]. Trends Plant Sci, 2007, 12(6): 239-244.
[3]	Zhang LH, You J, Chan ZL. Identification and characterization of TIFY family genes in Brachypodium distachyon [J]. J Plant Res, 2015, 128(6): 995-1005.
[4]	Chung HS, Howe GA. A critical role for the TIFY motif in repression of jasmonate signaling by a stabilized splice variant of the JASMONATE ZIM-domain protein JAZ10 in Arabidopsis [J]. Plant Cell, 2009, 21(1): 131-145.
[5]	Bai YH, Meng YJ, Huang DL, et al. Origin and evolutionary analysis of the plant-specific TIFY transcription factor family [J]. Genomics, 2011, 98(2): 128-136.
[6]	Shikata M, Takemura M, Yokota A, et al. Arabidopsis ZIM, a plant-specific GATA factor, can function as a transcriptional activator [J]. Biosci Biotechnol Biochem, 2003, 67(11): 2495-2497.
[7]	杨锐佳, 张中保, 吴忠义. 植物转录因子TIFY家族蛋白结构和功能的研究进展 [J]. 生物技术通报, 2020, 36(12): 121-128.
	Yang RJ, Zhang ZB, Wu ZY. Progress of the structural and functional analysis of plant transcription factor TIFY protein family [J]. Biotechnol Bull, 2020, 36(12): 121-128.
[8]	Chen Q, Dai R, Shuang S, et al. Genome-wide investigation of the TIFY transcription factors in alfalfa (Medicago sativa L.): identification, analysis, and expression [J]. BMC Plant Biol, 2024, 24(1): 840.
[9]	胡利宗, 李超琼, 张雯露, 等. 菜豆TIFY基因的全基因组鉴定与系统进化分析 [J]. 分子植物育种, 2020, 18(10): 3132-3140.
	Hu LZ, Li CQ, Zhang WL, et al. Genome-wide identification and phylogenetic analysis of the TIFY genes in common bean (Phaseolus vulgaris) [J]. Mol Plant Breed, 2020, 18(10): 3132-3140.
[10]	秦振芬, 孟祥霄, 温东, 等. 乌拉尔甘草TIFY基因家族鉴定及调控分析 [J]. 世界科学技术‒中医药现代化, 2022, 24(5): 1855-1864.
	Qin ZF, Meng XX, Wen D, et al. Genome-wide identification and regulatory analysis of the TIFY gene family in Glycyrrhiza uralensis [J]. Mod Tradit Chin Med Mater Med World Sci Technol, 2022, 24(5): 1855-1864.
[11]	王跃. 杨树TIFY基因家族的鉴定和CRISPR/Cas9技术敲除PtWRKY36基因的研究 [D]. 合肥: 安徽农业大学, 2018.
	Wang Y. Identification of TIFY gene family in poplar and the PtWRKY36 gene deletion using the technique of CRISPR/Cas9 gene editing [D]. Hefei: Anhui Agricultural University, 2018.
[12]	杨杰, 陈蓉, 胡文娟, 等. 甜橙TIFY基因家族的鉴定及其在成花诱导中的表达分析 [J]. 西北农业学报, 2022, 31(7): 866-875.
	Yang J, Chen R, Hu WJ, et al. Identification of TIIFY gene family in Citrus sinensis and its expression analysis in flowering induction [J]. Acta Agric Boreali Occidentalis Sin, 2022, 31(7): 866-875.
[13]	Xu L, Liu AL, Wang TY, et al. Characterization and coexpression analysis of the TIFY family genes in Euryale ferox related to leaf development [J]. Plants, 2023, 12(12): 2323.
[14]	徐亚楠, 吴新燕, 邸浩辰, 等. 茶树CsJAZ3基因的克隆表达及其与咖啡碱含量的相关性 [J]. 分子植物育种, 2023, 21(18): 5974-5981.
	Xu YN, Wu XY, Di HC, et al. Cloning and expression of CsJAZ3 gene in Camellia sinensis and its correlation with caffeine content [J]. Mol Plant Breed, 2023, 21(18): 5974-5981.
[15]	张月婷, 刘新亮, 戴小英, 等. 樟树TIFY转录因子家族鉴定与表达分析 [J]. 植物科学学报, 2024, 42(4): 466-477.
	Zhang YT, Liu XL, Dai XY, et al. Identification and expression analysis of the TIFY transcription factor family of Cinnamomum camphora (L.) Presl [J]. Plant Sci J, 2024, 42(4): 466-477.
[16]	Andrade Galan AG, Doll J, Saile SC, et al. The non-JAZ TIFY protein TIFY8 of Arabidopsis thaliana interacts with the HD-ZIP III transcription factor REVOLUTA and regulates leaf senescence [J]. Int J Mol Sci, 2023, 24(4): 3079.
[17]	Zhao XQ, He YQ, Liu YX, et al. JAZ proteins: Key regulators of plant growth and stress response [J]. Crop J, 2024, 12(6): 1505-1516.
[18]	温东, 王梦月, 米要磊, 等. 中药火麻仁基原植物大麻的TIFY基因家族鉴定及功能分析 [J]. 中国实验方剂学杂志, 2020, 26(24): 134-143.
	Wen D, Wang MY, Mi YL, et al. Genome-wide identification and characterization of TIFY gene family in medicinal plant Cannabis sativa [J]. Chin J Exp Tradit Med Formulae, 2020, 26(24): 134-143.
[19]	Guan YX, Zhang QY, Li MH, et al. Genome-wide identification and expression pattern analysis of TIFY family genes reveal their potential roles in Phalaenopsis aphrodite flower opening [J]. Int J Mol Sci, 2024, 25(10): 5422.
[20]	张晶星. 油松球花发育途径中PtMYB和PtJAZ关键基因鉴定及其调控网络构建 [D]. 北京: 北京林业大学, 2022.
	Zhang JX. Identification of PtMYB and PtJAZ key genes and the construction of regulatory network during development of Pinus tabuliformis carr. strobilis [D]. Beijing: Beijing Forestry University, 2022.
[21]	Trang Nguyen H, Thi Mai To H, Lebrun M, et al. Jasmonates-the master regulator of rice development, adaptation and defense [J]. Plants, 2019, 8(9): 339.
[22]	曲达. 棉花GhMYC2-like的互作蛋白筛选与鉴定 [D]. 郑州: 郑州大学, 2022.
	Qu D. Screening and identification of GhMYC2-like interacting proteins in Gossypium [D]. Zhengzhou: Zhengzhou University, 2022.
[23]	Wang Y, Li Y, He SP, et al. A cotton (Gossypium hirsutum) WRKY transcription factor (GhWRKY22) participates in regulating anther/pollen development [J]. Plant Physiol Biochem, 2019, 141: 231-239.
[24]	Guan YX, Ding L, Jiang JF, et al. The TIFY family protein CmJAZ1-like negatively regulates petal size via interaction with the bHLH transcription factor CmBPE2 in Chrysanthemum morifolium [J]. Plant J, 2022, 112(6): 1489-1506.
[25]	刘同金, 刘振洋, 班秋妍, 等. 丝瓜TIFY基因克隆及组织表达分析 [J]. 西北植物学报, 2024, 44(11): 1752-1759.
	Liu TJ, Liu ZY, Ban QY, et al. Cloning and tissue expression analysis of TIFY genes in Luffa cylindrica [J]. Acta Bot Boreali Occidentalia Sin, 2024, 44(11): 1752-1759.
[26]	王丽丽, 邓鸿, 于润润, 等. 甜樱桃TIFY基因家族全基因组鉴定及在果实发育中的表达分析 [J/OL]. 分子植物育种, 2024. .
	Wang LL, Deng H, Yu RR, et al. Genome-wide identification and expression enalysis of sweet cherry TIFY gene family in fruit development [J/OL]. Mol Plant Breed, 2024. .
[27]	Liu LM, Bai N, Zheng YQ, et al. Genome-wide identification and analysis of TIFY family in highbush blueberry and their responses to exogenous jasmonic acid [J]. Sci Hortic, 2022, 305: 111391.
[28]	Tao JJ, Jia HM, Wu MT, et al. Genome-wide identification and characterization of the TIFY gene family in kiwifruit [J]. BMC Genomics, 2022, 23(1): 179.
[29]	谢敏. 不同果形葡萄果实转录组分析及TIFY10A基因功能的初步分析 [D]. 兰州: 甘肃农业大学, 2021.
	Xie M. Transcriptome analysis of different fruit-shaped grape (Vitis vinifera L.) and function analysis of TIFY10A gene [D]. Lanzhou: Gansu Agricultural University, 2021.
[30]	刘良淼. VcTIFYs调控蓝莓果实大小机制研究 [D]. 金华: 浙江师范大学, 2023.
	Liu LM. Study on fruit-size regulation mechanism of bluebery TIFY members [D]. Jinhua: Zhejiang Normal University, 2023.
[31]	Nguyen DT, Hayes JE, Harris J, et al. Fine mapping of a vigor QTL in chickpea (Cicer arietinum L.) reveals a potential role for Ca4_TIFY4B in regulating leaf and seed size [J]. Front Plant Sci, 2022, 13: 829566.
[32]	姚雅鑫. 铭贤169小麦种子休眠与萌发相关基因的克隆和功能研究 [D]. 杨凌: 西北农林科技大学, 2023.
	Yao YX. Molecular cloning and functional study of genes related to seed dormancy and germination in Mingxian 169 wheat [D]. Yangling: Northwest A & F University, 2023.
[33]	Dong K, Wu FQ, Cheng SQ, et al. OsPRMT6a-mediated arginine methylation of OsJAZ1 regulates jasmonate signaling and spikelet development in rice [J]. Mol Plant, 2024, 17(6): 900-919.
[34]	谢思艺, 周承哲, 朱晨, 等. 茶树CsTIFY家族全基因组鉴定及非生物胁迫和激素处理中主要基因表达分析 [J]. 园艺学报, 2022, 49(1): 100-116.
	Xie SY, Zhou CZ, Zhu C, et al. Genome-wide identification and expression analysis of CsTIFY transcription factor family under abiotic stress and hormone treatments in Camellia sinensis [J]. Acta Hortic Sin, 2022, 49(1): 100-116.
[35]	Wang HR, Zhang Y, Zhang L, et al. Genome-wide identification and characterization of the TIFY gene family and their expression patterns in response to MeJA and aluminum stress in centipedegrass (Eremochloa ophiuroides) [J]. Plants, 2024, 13(3): 462.
[36]	Sheng Y, Yu H, Pan HF, et al. Genome-wide analysis of the gene structure, expression and protein interactions of the peach (Prunus persica) TIFY gene family [J]. Front Plant Sci, 2022, 13: 792802.
[37]	Lv GB, Han R, Shi JJ, et al. Genome-wide identification of the TIFY family reveals JAZ subfamily function in response to hormone treatment in Betula platyphylla [J]. BMC Plant Biol, 2023, 23(1): 143.
[38]	赵佳莹, 应宇鑫, 许张婷, 等. 掌叶覆盆子TIFY基因家族鉴定及表达分析 [J]. 核农学报, 2024, 38(10): 1823-1835.
	Zhao JY, Ying YX, Xu ZT, et al. Identification and expression profiles of TIFY gene family in Rcbus chingii Hu [J]. J Nucl Agric Sci, 2024, 38(10): 1823-1835.
[39]	练从龙, 兰金旭, 杨晶凡, 等. 基于冬凌草全长转录组的TIFY基因家族鉴定与表达分析 [J]. 福建农业学报, 2024, 39(3): 290-301.
	Lian CL, Lan JX, Yang JF, et al. Identification and expressions of TIFY family based on the full-length transcriptome in Isodon rubescens [J]. Fujian J Agric Sci, 2024, 39(3): 290-301.
[40]	张晶星, 马彦广, 王辉丽, 等. 油松JAZ基因家族特征及其与DELLA蛋白互作的功能域鉴定 [J]. 北京林业大学学报, 2022, 44(12): 12-22.
	Zhang JX, Ma YG, Wang HL, et al. Characteristics of JAZ gene family of Pinus tabuliformis and identification of functional domain of its interaction with DELLA protein [J]. J Beijing For Univ, 2022, 44(12): 12-22.
[41]	Zheng LL, Wan Q, Wang HG, et al. Genome-wide identification and expression of TIFY family in cassava (Manihot esculenta Crantz) [J]. Front Plant Sci, 2022, 13: 1017840.
[42]	胡睿, 郭建秀, 郭小强, 等. 铁皮石斛DoTIFY基因家族全基因组鉴定及在原球茎发育过程中的表达 [J]. 生物学杂志, 2021, 38(5): 53-58.
	Hu R, Guo JX, Guo XQ, et al. Genome-wide identification and analysis of the TIFY gene family in Dendrobium officinale Kimura et Migo during protocorm development [J]. J Biol, 2021, 38(5): 53-58.
[43]	于欣欣, 米要磊, 孟祥霄, 等. 人参TIFY基因家族鉴定与分析 [J]. 中华中医药学刊,2 022, 40(3): 72-76, 261-263.
	Yu XX, Mi YL, Meng XX, et al. Identification and analysis of the TIFY gene family in Panax ginseng [J]. Chinese J Tradit Chin Med, 2022, 40(3): 72-76, 261-263.
[44]	Li L, Liu YC, Huang Y, et al. Genome-wide identification of the TIFY family in Salvia miltiorrhiza reveals that SmJAZ3 interacts with SmWD40-170, a relevant protein that modulates secondary metabolism and development [J]. Front Plant Sci, 2021, 12: 630424.
[45]	孙浩文, 曲硕, 刘芳, 等. 大豆胞囊线虫3号生理小种抗性相关TIFY基因生物信息学分析 [J]. 大豆科学, 2023, 42(5): 532-544.
	Sun HW, Qu S, Liu F, et al. Bioinformatics analysis of TIFY genes related to resistance of soybean cyst nematode race 3 [J]. Soybean Sci, 2023, 42(5): 532-544.
[46]	曹凯, 卢佳锋, 周雅楠, 等. 茉莉酸响应列当寄生向日葵的相关差异表达基因分析 [J/OL]. 分子植物育种, 2025. .
	Cao K, Lu JF, Zhou YN, et al. Analysis of differential gene expression related to jasmonic acid response of broomrape parasitizing sunflower [J/OL]. Mol Plant Breed, 2025. .
[47]	梅闯, 张小燕, 闫鹏, 等. 苹果TIFY家族基因鉴定及其在虫害胁迫下的表达分析 [J]. 园艺学报, 2021, 48(2): 233-242.
	Mei C, Zhang XY, Yan P, et al. Identification of TIFY family in apple and their expression analysis under insect stress [J]. Acta Hortic Sin, 2021, 48(2): 233-242.
[48]	Singh P, Mukhopadhyay K. Comprehensive molecular dissection of TIFY transcription factors reveal their dynamic responses to biotic and abiotic stress in wheat (Triticum aestivum L.) [J]. Sci Rep, 2021, 11(1): 9739.
[49]	Sen S. Genome-wide TIFY family in Arachis hypogaea in the perspective of legume JAZs [J]. J Crop Sci Biotechnol, 2022, 25(4): 465-488.
[50]	Tong SY, Chen Y, Wei YY, et al. Genome-wide identification and response to exogenous hormones and pathogens of the TIFY gene family in Fragaria ananassa [J]. Plant Growth Regul, 2024, 103(3): 787-802.
[51]	Chung HS, Koo AJK, Gao XL, et al. Regulation and function of Arabidopsis JASMONATE ZIM-domain genes in response to wounding and herbivory [J]. Plant Physiol, 2008, 146(3): 952-964.
[52]	Zhang HF, Liu ZW, Geng RM, et al. Genome-wide identification of the TIFY gene family in tobacco and expression analysis in response to Ralstonia solanacearum infection [J]. Genomics, 2024, 116(3): 110823.
[53]	Sen S, DasGupta M. Involvement of Arachis hypogaea Jasmonate ZIM domain/TIFY proteins in root nodule symbiosis [J]. J Plant Res, 2021, 134(2): 307-326.
[54]	Gimenez-Ibanez S, Boter M, Ortigosa A, et al. JAZ2 controls stomata dynamics during bacterial invasion [J]. New Phytol, 2017, 213(3): 1378-1392.
[55]	Ghorbel M, Brini F, Sharma A, et al. Role of jasmonic acid in plants: the molecular point of view [J]. Plant Cell Rep, 2021, 40(8): 1471-1494.
[56]	Zhu ZQ, An FY, Feng Y, et al. Derepression of ethylene-stabilized transcription factors (EIN3/EIL1) mediates jasmonate and ethylene signaling synergy in Arabidopsis [J]. Proc Natl Acad Sci USA, 2011, 108(30): 12539-12544.
[57]	Chen LG, Zhang LP, Xiang SY, et al. The transcription factor WRKY75 positively regulates jasmonate-mediated plant defense to necrotrophic fungal pathogens [J]. J Exp Bot, 2021, 72(4): 1473-1489.
[58]	Yamada S, Kano A, Tamaoki D, et al. Involvement of OsJAZ8 in jasmonate-induced resistance to bacterial blight in rice [J]. Plant Cell Physiol, 2012, 53(12): 2060-2072.
[59]	Han Y, Luthe D. Key genes in the JAZ signaling pathway are up-regulated faster and more abundantly in caterpillar-resistant maize [J]. J Chem Ecol, 2022, 48(2): 179-195.
[60]	李秀青, 胡子曜, 雷建峰, 等. 棉花黄萎病抗性相关基因GhTIFY9的克隆与功能分析 [J]. 生物技术通报, 2022, 38(8): 127-134.
	Li XQ, Hu ZY, Lei JF, et al. Cloning and functional analysis of gene GhTIFY9 related to cotton Verticillium wilt resistance [J]. Biotechnol Bull, 2022, 38(8): 127-134.
[61]	He JC, Cheng YK, Xiao LD, et al. Comprehensive identification and expression analysis of the TIFY gene family in cucumber [J]. Not Bot Horti Agrobo, 2022, 50(2): 12703.
[62]	Dai ZN, Dong SY, Miao H, et al. Genome-wide identification of TIFY genes and their response to various pathogen infections in cucumber (Cucumis sativus L.) [J]. Sci Hortic, 2022, 295: 110814.
[63]	Liu K, Xu HY, Gao XB, et al. Pan-genome analysis of TIFY gene family and functional analysis of CsTIFY genes in cucumber [J]. Int J Mol Sci, 2024, 25(1): 185.
[64]	袁晓, 蒋园园, 朱云娜, 等. JAZ家族基因在采后黄瓜低温贮藏条件下的表达分析 [J]. 浙江农业学报, 2024, 36(8): 1820-1831.
	Yuan X, Jiang YY, Zhu YN, et al. Expression analysis of JAZ family genes in harvested cucumber fruit under cold storage condition [J]. Acta Agric Zhejiangensis, 2024, 36(8): 1820-1831.
[65]	李国华, 罗磊, 穆淑媛, 等. 青檀JAZ基因的鉴定及在低温胁迫下的表达 [J]. 分子植物育种, 2026, 24(14): 4612-4618.
	Li GH, Luo L, Mu SY, et al. Identification of JAZ genes in Pteroceltis tatarinowii and their expression under low temperature stress [J]. Mol Plant Breed, 2026, 24(14): 4612-4618.
[66]	Wang XD, Li N, Zan TX, et al. Genome-wide analysis of the TIFY family and function of CaTIFY7 and CaTIFY10b under cold stress in pepper (Capsicum annuum L.) [J]. Front Plant Sci, 2023, 14: 1308721.
[67]	Li GH, Manzoor MA, Chen R, et al. Genome-wide identification and expression analysis of TIFY genes under MeJA, cold and PEG-induced drought stress treatment in Dendrobium huoshanense [J]. Physiol Mol Biol Plants, 2024, 30(4): 527-542.
[68]	车莉莉. 茉莉酸甲酯诱导VvJAZ13参与葡萄抗寒的分子机制研究 [D]. 兰州: 甘肃农业大学, 2024.
	Che LL. Molecular mechanism of methyl jasmonate-induced VvJAZ13 involved in cold resistance in grape [D]. Lanzhou: Gansu Agricultural University, 2024.
[69]	Lv Y, Yang M, Hu D, et al. The OsMYB30 transcription factor suppresses cold tolerance by interacting with a JAZ protein and suppressing β-amylase expression [J]. Plant Physiol, 2017, 173(2): 1475-1491.
[70]	Hu YR, Jiang LQ, Wang F, et al. Jasmonate regulates the inducer of cbf expression-C-repeat binding factor/DRE binding factor1 cascade and freezing tolerance in Arabidopsis [J]. Plant Cell, 2013, 25(8): 2907-2924.
[71]	李晓静, 鲁海菊, 岳亮亮. 石榴TIFY基因家族的全基因组鉴定与表达分析 [J]. 江苏农业科学, 2024, 52(19): 41-50.
	Li XJ, Lu HJ, Yue LL. Whole genome identification and expression analysis of TIFY gene family in Punica granatum L. [J]. Jiangsu Agric Sci, 2024, 52(19): 41-50.
[72]	袁芳玉. 石榴TIFY全基因组鉴定及表达分析 [D]. 合肥: 安徽农业大学, 2024.
	Yuan FY. Genome-wide identification and analysis of the TIFY gene family in Punica granatum and expression analysis [D]. Hefei: Anhui Agricultural University, 2024.
[73]	沙伟, 卫杰, 张梅娟, 等. 砂藓干旱胁迫相关转录因子基因RcTIFY1的克隆及表达分析 [J]. 分子植物育种, 2023, 21(9): 2925-2932.
	Sha W, Wei J, Zhang MJ, et al. Cloning and expression analysis of the drought stress related transcription factor gene RcTIFY1 of Racomitrium canescens [J]. Mol Plant Breed, 2023, 21(9): 2925-2932.
[74]	赵晓晓, 谢坤良, 张舒梦, 等. 柳枝稷TIFY基因家族的鉴定与分析 [J]. 草地学报, 2019, 27(5): 1126-1137.
	Zhao XX, Xie KL, Zhang SM, et al. Identification and analysis of TIFY gene family of Panicum virgatum [J]. Acta Agrestia Sin, 2019, 27(5): 1126-1137.
[75]	李娜. 谷子干旱胁迫下转录组分析和TIFY基因家族分析 [D]. 太谷: 山西农业大学, 2020.
	Li N. Transcriptome analysis under drought stress and TIFY gene family analysis of Setaria italica [D]. Taigu: Shanxi Agricultural University, 2020.
[76]	Li SH, Li JY, Li DL, et al. Genome-wide identification of the eggplant jasmonate ZIM-domain (JAZ) gene family and functional characterization of SmJAZ10 in modulating chlorophyll synthesis in leaves [J]. Int J Biol Macromol, 2024, 283: 137804.
[77]	Zhang SP, Zheng DY, Gao YQ, et al. The TIFY transcription factor ZmJAZ13 enhances plant tolerance to drought and salt stress by interacting with ZmbHLH161 and ZmA0A1D6GLB9 [J]. Plant Sci, 2025, 352: 112388.
[78]	Zhao JQ, Li AJ, Xu M, et al. Genome-wide analysis of the TIFY family in Lycium and the negative regulation of stomatal development by LrJAZ2 gene [J]. Plant Physiol Biochem, 2024, 206: 108285.
[79]	Zhao ZX, Meng GH, Zamin I, et al. Genome-wide identification and functional analysis of the TIFY family genes in response to abiotic stresses and hormone treatments in Tartary buckwheat (Fagopyrum tataricum) [J]. Int J Mol Sci, 2023, 24(13): 10916.
[80]	Wang Y, Li N, Zhan JP, et al. Genome-wide analysis of the JAZ subfamily of transcription factors and functional verification of BnC08.JAZ1-1 in Brassica napus [J]. Biotechnol Biofuels Bioprod, 2022, 15(1): 93.
[81]	Zhao PY, Zheng JX, Xu QY, et al. Genome-wide identification and analysis of the JAZ gene family in rapeseed reveal JAZ2 and JAZ3 roles in drought and salt stress tolerance [J]. J Agric Food Chem, 2025, 73(33): 20955-20971.
[82]	祁稼民, 许春苗, 肖斌. 马铃薯TIFY基因家族的全基因组鉴定及表达分析 [J]. 作物学报, 2024, 50(9): 2297-2309.
	Qi JM, Xu CM, Xiao B. Genome-wide identification and expression analysis of TIFY gene family in potato (Solanum tuberosum L.) [J]. Acta Agron Sin, 2024, 50(9): 2297-2309.
[83]	Pu ZF, Qin TY, Wang YH, et al. Genome-wide analysis of the JAZ gene family in potato and functional verification of StJAZ23 under drought stress [J]. Int J Mol Sci, 2025, 26(5): 2360.
[84]	Fu J, Wu H, Ma SQ, et al. OsJAZ1 attenuates drought resistance by regulating JA and ABA signaling in rice [J]. Front Plant Sci, 2017, 8: 2108.
[85]	Seo JS, Joo J, Kim MJ, et al. OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice [J]. Plant J, 2011, 65(6): 907-921.
[86]	Wu YY, Sun Y, Wang WM, et al. OsJAZ10 negatively modulates the drought tolerance by integrating hormone signaling with systemic electrical activity in rice [J]. Plant Physiol Biochem, 2024, 211: 108683.
[87]	Lian CL, Zhang B, Li JJ, et al. Genome-wide identification, characterization and expression pattern analysis of TIFY family members in Artemisia argyi [J]. BMC Genomics, 2024, 25(1): 925.
[88]	巩永永, 端木慧子. 甜菜TIFY基因家族的全基因组鉴定与生物信息学分析 [J]. 中国农学通报, 2022, 38(8): 17-24.
	Gong YY, Duanmu HZ. TIFY gene family in sugar beet: whole genome identification and bioinformatics analysis [J]. Chin Agric Sci Bull, 2022, 38(8): 17-24.
[89]	Liu XJ, Yu FY, Yang GY, et al. Identification of TIFY gene family in walnut and analysis of its expression under abiotic stresses [J]. BMC Genomics, 2022, 23(1): 190.
[90]	刘梦迪, 倪守飞, 王月雪, 等. 大麦TIFY基因家族成员鉴定及表达分析 [J]. 南方农业学报, 2022, 53(2): 417-429.
	Liu MD, Ni SF, Wang YX, et al. Identification and expression analysis of TIFY gene family members in barley [J]. J South Agric, 2022, 53(2): 417-429.
[91]	吴媛, 苏世贤, 尤春生, 等. 辣椒TIFY基因家族的鉴定与分析 [J]. 种子, 2023, 42(3): 1-10.
	Wu Y, Su SX, You CS, et al. Identification and analysis of TIFY gene family in pepper [J]. Seed, 2023, 42(3): 1-10.
[92]	王昊宁, 周佩, 刘燕, 等. 月季TIFY基因家族的全基因组分析 [J]. 河南农业大学学报, 2022, 56(6): 990-997, 1060.
	Wang HN, Zhou P, Liu Y, et al. Whole-genome analysis of the TIFY gene family in Rosa chinensis Jacq. [J]. J Henan Agric Univ, 2022, 56(6): 990-997, 1060.
[93]	Sun H, Chen L, Li JY, et al. The JASMONATE ZIM-domain gene family mediates JA signaling and stress response in cotton [J]. Plant Cell Physiol, 2017, 58(12): 2139-2154.
[94]	Li X, Li CJ, Shi L, et al. Jasmonate signaling pathway confers salt tolerance through a NUCLEAR FACTOR-Y trimeric transcription factor complex in Arabidopsis [J]. Cell Rep, 2024, 43(3): 113825.
[95]	Wu H, Ye HY, Yao RF, et al. OsJAZ9 acts as a transcriptional regulator in jasmonate signaling and modulates salt stress tolerance in rice [J]. Plant Sci, 2015, 232: 1-12.
[96]	Liu YL, Zheng L, Jin LG, et al. Genome-wide analysis of the soybean TIFY family and identification of GmTIFY10e and GmTIFY10g response to salt stress [J]. Front Plant Sci, 2022, 13: 845314.
[97]	Zhao CY, Pan XW, Yu Y, et al. Overexpression of a TIFY family gene, GsJAZ2, exhibits enhanced tolerance to alkaline stress in soybean [J]. Mol Breed, 2020, 40(3): 33.
[98]	Zhang C, Yang RJ, Zhang TT, et al. ZmTIFY16, a novel maize TIFY transcription factor gene, promotes root growth and development and enhances drought and salt tolerance in Arabidopsis and Zea mays [J]. Plant Growth Regul, 2023, 100(1): 149-160.
[99]	陈运梁. 外源茉莉酸甲酯对小桐子耐盐性和渗透调节的影响及其TIFY家族的鉴定 [D]. 昆明: 云南师范大学, 2024.
	Chen YL. Effects of exogenous methyl jasmonate on salt tolerance and osmoregulation in Jatropha curcas and identification of its TIFY family [D]. Kunming: Yunnan Normal University, 2024.
[100]	刘永翔. 核桃TIFY基因家族的鉴定及分析 [D]. 杨凌: 西北农林科技大学, 2023.
	Liu YX. Genome-wide identification and analysis of the TIFY family in walnut [D]. Yangling: Northwest A & F University, 2023.

物种Species	基因Gene	功能Function	参考文献 Reference
拟南芥 Arabidopsis thaliana	TIFY8	叶绿素损失减缓、光系统II功能维持更久，延缓叶片衰老进程	［16］
甘蓝型油菜 Brassica napus	BnaJAZ2 BnaJAZ3	BnaJAZ2正向调控耐旱性 BnaJAZ3负向调控耐盐性	［81］
辣椒 Capsicum annuum	CaTIFY7 CaTIFY10b	提高植物叶片的活性氧含量，正向调控辣椒对低温胁迫的抗性	［66］
菊花 Chrysanthemum morifolium	CmJAZ1-like CmBPE2	CmJAZ1-like通过与CmBPE2相互作用，从而共同影响花瓣大小	［24］
鹰嘴豆 Cicer arietinum	Ca4-TIFY4B	调控植株活力相关性状（叶片大小、种子大小）	［31］
黄瓜 Cucumis sativus	CsJAZ2 CsJAZ6， CsZML2	过表达植株中ROS清除系统的POD、SOD和CAT酶活性显著降低，负调控黄瓜对灰霉病的抗性	［62］
苦荞 Fagopyrum tataricum	FtJAZ10， FtJAZ12	抑制热诱导的细胞死亡，提高烟草对热胁迫的耐受能力	［79］
大豆 Glycine max	GmTIFY10e， GmTIFY10g	主根长度和鲜重均显著增高，正向调控植物的盐胁迫耐受性	［96］
陆地棉 Gossypium hirsutum	GhTIFY9	棉花抗黄萎病反应的正向调控因子，增强棉花对黄萎病菌的敏感性	［60］
枸杞 Lycium barbarum	LrJAZ2	气孔密度和气孔指数显著降低，叶片失水率下降，负调控植物气孔发育	［78］
烟草 Nicotiana tabacum	NtTIFY7	NtTIFY7 沉默植株对青枯病的敏感性显著增强。正向调控烟草对青枯病的抗性	［52］
茄子 Solanum melongena	SmJAZ10	负向调控叶片叶绿素合成	［76］
丹参 Salvia miltiorrhiza	SmWD40-170	促进根系生长和次生代谢物积累，调节气孔运动来应对干旱胁迫	［44］
马铃薯 Solanum tuberosum	StJAZ23	促进根系发育，增强根系活力，提升抗氧化能力，调控胁迫相关激素积累	［83］
蓝莓 Vaccinium corymbosum	VcTIFY6B， VcTIFY9， VcTIFY10A	增加果实的横径、单果质量及种子数目，调控果实发育	［30］
葡萄 Vitis vinifera	VvTIFY10A	促进果实纵向生长、抑制横向生长，对果实形状产生调控作用	［29］
玉米 Zea mays	ZmJAZ13	调节次生代谢产物和酶活性，增强植物对干旱和盐胁迫的耐受性	［77］

物种Species	基因Gene	功能Function	参考文献 Reference
拟南芥 Arabidopsis thaliana	TIFY8	叶绿素损失减缓、光系统II功能维持更久，延缓叶片衰老进程	［16］
甘蓝型油菜 Brassica napus	BnaJAZ2 BnaJAZ3	BnaJAZ2正向调控耐旱性 BnaJAZ3负向调控耐盐性	［81］
辣椒 Capsicum annuum	CaTIFY7 CaTIFY10b	提高植物叶片的活性氧含量，正向调控辣椒对低温胁迫的抗性	［66］
菊花 Chrysanthemum morifolium	CmJAZ1-like CmBPE2	CmJAZ1-like通过与CmBPE2相互作用，从而共同影响花瓣大小	［24］
鹰嘴豆 Cicer arietinum	Ca4-TIFY4B	调控植株活力相关性状（叶片大小、种子大小）	［31］
黄瓜 Cucumis sativus	CsJAZ2 CsJAZ6， CsZML2	过表达植株中ROS清除系统的POD、SOD和CAT酶活性显著降低，负调控黄瓜对灰霉病的抗性	［62］
苦荞 Fagopyrum tataricum	FtJAZ10， FtJAZ12	抑制热诱导的细胞死亡，提高烟草对热胁迫的耐受能力	［79］
大豆 Glycine max	GmTIFY10e， GmTIFY10g	主根长度和鲜重均显著增高，正向调控植物的盐胁迫耐受性	［96］
陆地棉 Gossypium hirsutum	GhTIFY9	棉花抗黄萎病反应的正向调控因子，增强棉花对黄萎病菌的敏感性	［60］
枸杞 Lycium barbarum	LrJAZ2	气孔密度和气孔指数显著降低，叶片失水率下降，负调控植物气孔发育	［78］
烟草 Nicotiana tabacum	NtTIFY7	NtTIFY7 沉默植株对青枯病的敏感性显著增强。正向调控烟草对青枯病的抗性	［52］
茄子 Solanum melongena	SmJAZ10	负向调控叶片叶绿素合成	［76］
丹参 Salvia miltiorrhiza	SmWD40-170	促进根系生长和次生代谢物积累，调节气孔运动来应对干旱胁迫	［44］
马铃薯 Solanum tuberosum	StJAZ23	促进根系发育，增强根系活力，提升抗氧化能力，调控胁迫相关激素积累	［83］
蓝莓 Vaccinium corymbosum	VcTIFY6B， VcTIFY9， VcTIFY10A	增加果实的横径、单果质量及种子数目，调控果实发育	［30］
葡萄 Vitis vinifera	VvTIFY10A	促进果实纵向生长、抑制横向生长，对果实形状产生调控作用	［29］
玉米 Zea mays	ZmJAZ13	调节次生代谢产物和酶活性，增强植物对干旱和盐胁迫的耐受性	［77］