Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (10): 142-147.

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Investigation on Diversity of Endophytic Bacterial Community in Xisha Wild Noni（Morinda citrifolia L.）Seed

Liu Yang1,Li Hui1,Li Jinxia1,Cao Yanhua1,Yao Su1,Bai Feirong1,Tan Wangqiao2, Cheng Chi1,   

  1. （1. China Center of Industrial Culture Collection，China National Research Institute of Food and Fermentation Industries，Beijing 100027 ； 2. Hainan Noni Biological Engineering Development Co.，Ltd.，Haikou 570125）
  • Received:2013-03-28 Revised:2013-10-15 Online:2013-10-14 Published:2013-10-15

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Abstract:

Using the 16S rDNA clone library technique, a priliminary study was conducted on the community diversity of endophytic bacteria in seeds of Xisha wild Noni. The results indicated that the endophytic bacterial communities contained 42 Operational Taxonomic Units （OTUs）in their clone libraries respectively, involving 33 genus from 4 groups of Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria.
The dominant bacterium（abundance）were Enterobacter sp.（17.55%）, Bacillus sp.（16.79%）, Acinetobacter sp.（6.11%）, Pseudomonas sp.（6.11%）and Piscinibacter sp.（6.11%）. This was the first report of the study on endophytic bacterial communities in the Noni seeds with culture-independent method.

Key words: Noni, Seed, Endophytic bacteria, Bacterial community, Culture-independent method