Advances in Directed Research of VD3 Hydroxylase

Abstract

Abstract: Vitamin D₃ hydroxylase is a cytochrome P450(CYP)responsible for the biocatalytic conversion of vitamin D3 to 1α, 25dihydroxyvitamin D3(1α, 25(OH)₂VD3)。Since VD₃ is not a natural substrate for Vdh, the hydroxylase of Vdh is quite low. Researchers construct a novel of recombination Vdh plasmid by directed evolution to optimize the active sites of catalytic structure, thereby improving the hydroxylase ability. This article reviewed Vdh structure properties and development of directed evolution.

Key words: Vdh Heterologous expression, Redox partner protein, Directed evolution, 25-hydroxyvitaminD3 1α, 25- dihyroxyvitamin D3

Liu Miao, Wang Yonghui, Lu Qun. Advances in Directed Research of VD₃ Hydroxylase[J]. Biotechnology Bulletin, 2014, 0(1): 27-31.

References

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Advances in Directed Research of VD₃ Hydroxylase

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