RNAi Technology and Its Application in Fungal Gene Functional Studies

doi:10.13560/j.cnki.biotech.bull.1985.2015.08.008

Abstract

Abstract: RNA interference (RNAi) is a phenomenon of post-transcriptional gene silencing triggered by highly conserved exogenous or endogenous double-strand RNA (dsRNA) during the evolution. RNAi, as an efficient and specific gene blocking technology, is widely applied to gene functional studies and the areas of treating infectious diseases and malignant tumors. RNAi has become one of the hotspots in the 21st century. This paper reviewed the several aspects of RNAi, including the history of discovering it, mechanism, characteristics, and the application prospects in fungal gene functional analysis, which may provide the theoretical basis for related researches.

Key words: RNA interference, gene silencing, fungi, gene function

Su Zijing, Li Qiaoling, Huang Cheng, Xie Chengjian, Yang Xingyong. RNAi Technology and Its Application in Fungal Gene Functional Studies[J]. Biotechnology Bulletin, 2015, 31(8): 50-58.

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