Isolation and Identification of Saline-alkaline Tolerant Hydrocarbon-degrading Strains and Study on Their Saline-alkaline Tolerant Characteristics

doi:10.13560/j.cnki.biotech.bull.1985.2015.01.023

Abstract

Abstract: 10 saline-alkaline tolerant strains which can degrade hydrocarbons were isolated from a petroleum-contaminated saline soil in Dagang oilfield of China. According to their morphology, physiochemical characteristics and 16S rRNA sequence analysis, the 10 strains were identified as Ochrobactrum, Staphylococcus, Dietzia, Corynebacterium, Achromobacter, Microbacterium and Bacillus. Moreover, a series of liquid incubation experiments were conducted to investigate their saline tolerant and alkaline tolerant characteristics. The salt resistance test demonstrated that 10 strains grew well at NaCl concentrations ranging from 0.5% to 5.0% or even higher except B07 which could grow under 3% of NaCl. B02 and B05 still had high growth activity under a salinity of 11%. The alkalinity resistance test demonstrated that all the 10 strains grew well at pH 9. Besides, B01, B03, B04, B06 and B09 could endure the pH of 10, B03 and B04 still had high growth activity with the pH of 11. The results indicated that petroleum-degradation bacteria with high saline tolerant and alkaline tolerant abilities were widespread among different microbial species. Therefore, they are expected to be widely used in the remediation of oil-contaminated saline alkaline soil.

Key words: saline-alkaline tolerance, petroleum contamination, soil, petroleum-degradation bacteria, bioremediation

Zhang Hairong, Tang Jingchun, Sun Kejing, Zhang Qingmin. Isolation and Identification of Saline-alkaline Tolerant Hydrocarbon-degrading Strains and Study on Their Saline-alkaline Tolerant Characteristics[J]. Biotechnology Bulletin, 2015, 31(1): 151-159.

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