[1] James C. Global status of commercialized biotech/GM crops:2012[R] . International Service for the Acquisition of Agri-biotech Applications ISAAA, 2013. [2] Kapusi E, Hensel G, Coronado MJ, et al. The elimination of a selectable marker gene in the doubled haploid progeny of co-transformed barley plants[J]. Plant Molecular Biology, 2013, 81(1-2):149-160. [3] 郭敏亮, 诸庆, 高佃坤. 产生无标记农杆菌突变体方法的建立及优化[J]. 生物化学与生物物理进展, 2009(5):556-565. [4] 单晓呋, 李蓓, 张举仁. 利用FLP/frt重组系统产生无选择标记的转基因烟草植株[J]. 生物工程学报, 2006, 22(5):744-750. [5] 王根平, 杜文明, 夏兰琴. 植物安全转基因技术研究现状与展望[J]. 中国农业科学, 2014, 47(5):823-843. [6] Komari T, Hiei Y, Saito Y, et al. Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacte-rium tumefaciens and segregation of transformants free from selection markers[J]. Plant J, 1996, 10(1):165-174. [7] Miller M, Tagliani L, Wang N, et al. High efficiency transgene segregation in co-transformed maize plants using an Agrobacterium tumefaciens 2 T-DNA binary system[J]. Transgenic Research, 2002, 11(4):381-396. [8] Romano A, Raemakers K, Bernardi J, et al. Transgene organisation in potato after particle bombardment-mediated(co-)transformation using plasmids and gene cassettes[J]. Transgenic Research, 2003, 12(4):461-473. [9] Shiva Prakash N, Bhojaraja R, Shivbachan SK, et al. Marker-free transgenic corn plant production through co-bombardment[J]. Plant Cell Reports, 2009, 28(11):1655-1668. [10] Andrew PG, Cleofe N. Transposition mediated Re-positioning and subsequent elimination of marker genes from transgenic tomato[J]. Bio/Technology, 1993, 11(11):1286-1292. [11] Yoder JI, Goldsbrough AP, et al. Transformation systems for generating marker-free transgenic plants[J]. Bio/Technology, 1994, 12(3):263-267. [12] 洪林, 闫晓红, 刘正富, 等. A c/Ds系统及其在油菜突变体库构建中的应用策略[J]. 南方农业学报, 2012, 43(6):738-743. [13] Krishnaswamy L, Zhang JB, Peterson T. Fusion of reverse-oriented Ds termini following abortive transposition in Arabidopsis:implic-ations for the mechanism of Ac/Ds transposition[J]. Plant Cell Reports, 2010, 29(4):413-417. [14] 赵爱春, 龙定沛, 谭兵, 等. FLP/FRT位点特异性重组系统在高等真核生物中的研究进展[J]. 中国农业科学, 2011, 44(15):3252-3263. [15] Li B, Li N, Duan X, et al. Generation of marker-free transgenic maize with improved salt tolerance using the FLP/FRT recombination system[J]. J Biotechnol, 2010, 145(2):206-213. [16] Fladung M, Schenk TMH, Polak O, et al. Elimination of marker genes and targeted integration via FLP/FRT recombination system from yeast in hybrid aspen(Populus tremula L.×P. tremuloides Michx. )[J]. Tree Genetics and Genomes, 2010, 6(2):205-217. [17] Cregg JM, Madden KR. Use of site-specific recombination to regenerate selectable markers[J]. Molecular and General Genetics, 1989, 219(1-2):320-323. [18] Khan R, Nakamura I, Mii M. Development of disease-resistant marker-free tomato by R/RS site-specific recombination[J]. Plant Cell Reports, 2011, 30(6):1041-1053. [19] Saelim L, Phansiri S, Suksangpanomrung M, et al. Evaluation of a morphological marker selection and excision system to generate marker-free transgenic cassava plants[J]. Plant Cell Reports, 2009, 28(3):445-455. [20] Sugita K, Matsunaga E, Ebinuma H. Effective selection system for generating marker-free transgenic plants independent of sexual crossing[J]. Plant Cell Reports, 1999, 18(11):941-947. [21] Hirano N, Muroi T, Takahashi H, et al. Site-specific recombinases as tools for heterologous gene integration[J]. Applied Microbiology and Biotechnology, 2011, 92(2):227-239. [22] Zuo J, Niu QW, Moller SG, et al. Chemical-regulated, site-specific DNA excision in transgenic plants[J]. Nature Biotechnology, 2001, 19(2):157-161. [23] 杨平, 蔡小宁, 钟小仙. 拟南芥AtNHX1基因克隆和cre(lox)植物表达载体构建[J]. 江苏农业科学, 2007(6):356-358. [24] 郭霞, 李莉, 张毅, 等. 根癌农杆菌介导Bt基因转化水稻的研究[J]. 生物学杂志, 2009, 26(2):21-23. [25] Khattri A, Nandy S, Srivastava V, et al. Heat-inducible Cre-lox system for marker excision in transgenic rice[J]. J Biosci, 2011, 36(1):37-42. [26] 芦春斌, 高忱. 重叠PCR合成HPV16 E6、E7基因并构建植物表达双元载体[J]. 生物技术通报, 2011(4):143-146. [27] 尚玉花, 李莉, 陆徐忠, 等. 利用Cre/loxP系统删除转Bt基因水稻中的选择标记基因[J]. 生物学杂志, 2011, 28(2):5-8. [28] Chong-Perez B, Kosky RG, Reyes M, et al. Heat shock induced excision of selectable marker genes in transgenic banana bythe Cre-lox site-specific recombination system[J]. J Biotechnol, 2012, 159(4):265-273. [29] Maeser S, Kahmann R. The Gin recombinase of phage Mu can catalyse site-specific recombination in plant protoplasts[J]. Mol Gen Genet, 1991, 230(1-2):170-176. [30] 杨东生, 张研, 张建会, 等. Cre/Loxp重组酶系统与转基因[J]. 安徽农业科学, 2010, 38(8):3916-3918. [31] Zou X, Peng A, Xu L, et al. Efficient auto-excision of a selectable marker gene from transgenic citrus by combining the Cre/loxP system and ipt selection[J]. Plant Cell Reports, 2013, 32(10):1601-1613. [32] García-Almodóvar RC, Petri C, Gonzale-Padilla IMG, et al. Combination of site-specific recombination and a conditional selective marker gene allows for the production of marker-free tobacco plants[J]. Plant Cell, Tissue and Organ Culture, 2014, 116(2):205-215. [33] Righetti L, Djennane S, Berthelot P, et al. Elimination of the nptII marker gene in transgenic apple and pear with a chemically inducible R/Rs recombinase[J]. Plant Cell, Tissue and Organ Culturce, 2014, 117(3):335-348. [34] 邹丽婷, 宋洪元. 噬菌体phiC31位点重组酶系统在植物转基因中的应用[J]. 安徽农业科学, 2014, 42(5):1298-1301. [35] Ebinuma H, Nanto K, Kasahara S, et al. Marker-free gene targeting by recombinase-mediated cassette exchange[J]. Methods Mol Biol, 2012, 847:379-390. [36] Luo KM, Duan H, Zhao DG, et al. GM-gene-deletor:fused loxP-FRT recognition sequences dramatically improve the efficiency of FLP or CRE recombinase on transgene excision from pollen and seed of tobacco plants[J]. Plant Biotechnol J, 2007, 5:263-374. [37] 邹璐琳, 魏建华, 王宏芝, 等. 高效删除标记基因的Bhlea2植物表达载体的构建[J]. 生物技术通报, 2011(11):79-82. [38] Nandy S, Srivastava V. Marker-free site-specific gene integration in rice based on the use of two recombination systems[J]. Plant Biotechnology Journal, 2012, 10(8):904-912. [39] 晁岳恩, 吴政卿, 杨会民, 等. 11种植物psbA基因的密码子偏好性及聚类分析[J]. 核农学报, 2011, 25(5):927-932. [40] 巩智刚, 周海鹏, 徐芳, 等. 叶绿体转化及其应用于作物改良研究的最新进展[J]. 核农学报, 2012, 26(2):288-294. [41] Day A, Goldschmidt-Clermont M. The chloroplast transformation toolbox:selectable markers and marker remova[J]. Plant Biotechnology Journal, 2011, 9(5):540-553. |