Molecular ID Establishment of 13 Chinese Newly-developed Grape Cultivars

doi:10.13560/j.cnki.biotech.bull.1985.2016.04.018

Abstract

Abstract: Using 13 newly-developed grape cultivars as experimental materials，the phylogenetic relationship among them were analyzed and their molecular IDs were established，which aimed to provide the references for identifying the grape cultivar and protecting the new ones. SSR analysis was carried out on the tested grape cultivars with the international commonly-used 9 pairs of primers，and the codes of each cultivar was assigned according to the molecular weight of the amplified band of different cultivar by the primer. The results showed：a total of 48 alleles and an average of 5.3 alleles per primer were detected by 9 pairs of primers. The expected heterozygosity varied between 0.435-0.811，and the information content of polymorphism ranged from 0.401 to 0.784. After alleles assignment and coded，the molecular IDs of cultivars were established，by which the 13 grape cultivars can be identified. According to the clustering analysis，13 cultivars showed clear separations due to their different parents.

Key words: grape, new cultivars, SSR, molecular ID

WANG Hui-ling， ,YAN Ai-ling， ,SUN Lei， ,ZHANG Guo-jun, WANG Xiao-yue ,XU Hai-ying. Molecular ID Establishment of 13 Chinese Newly-developed Grape Cultivars[J]. Biotechnology Bulletin, 2016, 32(4): 137-142.

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