Abstract: Brewing grape wine is a process involved by many microorganisms that generally come from the grape berries if without exogenous inoculation. The high-throughput sequencing technology was used to analyze the microbial communities on the surface of different cultivars of wine grapes in Shacheng,China,which aims to understand the quality of the wine grapes,and provide the theoretical evidences for investigating the influences of microorganisms on the quality of brewing. The results showed that the richest microbial communities among these wine grapes were Riesling no matter of bacteria or fungi. The bacteria mainly were distributed in 8 phyla of Proteobacteria,Actinobacteria,Firmicutes,etc.,and 6 dominant genera of Erwinia,Pseudomonas,Bacillus,and so on. The 3 phyla of the fungi were Ascomycota,Basidiomycota,and Zygomycota;and the 9 genera of fungi were Alternaria,Cladosporium,Phoma,Fusarium,etc. The most similar species of the bacterial core OTUs were Arthrobacter sp. and Pseudomonas sp.,as well as Cladosporium sp.,Phoma sp.,Alternaria sp. and a lot of unknown ones for the fungal core OTUs. This study illustrates that the cultivar is the most important factor affecting the microbial communities. And from the results,it is inferred that the microorganisms on the surfaces of the wine grapes may be beneficial or adverse to the health of the grape vine,the quality of the grape berries and the process of wine brewing.

Key words: wine grape, high-throughput sequencing, microbial community, grape wine quality, bacterial diversity, fungal diversity