Toxic Effects of Four Currently-used Pesticides on Zebrafish Embryonic Development

doi:10.13560/j.cnki.biotech.bull.1985.2017-0032

Abstract

Abstract: In order to study the effects of four pesticides（fenbuconazole,pyraclostrobin,metoxazon,and pencycurone）,either individually or jointly,on the development of zebrafish embryos,seven experiments（blank control,solvent control,individual of 4 pesticides,and jointly combined four pesticides）were designed to treat zebrafish embryos for 72 h. The results showed that individual exposure to any of 4 pesticides at 50 ng/L led to the significant elevation on the rate of pericardial and yolk sac edema and the frequency of dorsal curvature in the larvae compared to the control. The exposure to fenbuconazole,metoxazon,and combined four pesticides resulted in a significant reduction in the heart rate and a significant increase in cardiac arrhythmia in the zebrafish larvae. The activities of Na⁺/K⁺-ATPase and Ca²⁺-ATPase of the embryos were significantly induced by the individual exposure to 4 pesticides,while the both activities were remarkably inhibited by the combined exposure. The results confirmed that these pesticides at environmental relevant concentration presented the clear toxicity to the development of fish embryo.

Key words: fenbuconazole, pyraclostrobin, metoxazon, pencycurone, combined exposure, Zebrafish, development of embryo

WU Yu-qiong, CHEN Ying, HU Yong-le, ZUO Zheng-hong, WANG Chong-gang. Toxic Effects of Four Currently-used Pesticides on Zebrafish Embryonic Development[J]. Biotechnology Bulletin, 2017, 33(6): 155-161.

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