Identification and Biological Characteristics of One Antagonistic Fungal Strain Against Sclerotinia sclerotiorum

doi:10.13560/j.cnki.biotech.bull.1985.2018-0073

Abstract

Abstract: Our aims are to isolate and select antagonistic endophytic fungi against Sclerotinia sclerotionum from the roots of Liparis japonica（Orchidaceae）,to identify their taxonomic status and study the characteristics of in vitro culturing it. Conventional PDA culture technology was applied to isolate and culture endophytic fungi and plate confrontation method to screen those strains with strong antagonistic activities against S. sclerotionum and to identify their taxonomic status,as well as the biological characteristics were studied under different carbon sources,nitrogen sources,temperature and pH values. One strongly antagonistic endophytic fungus against S. sclerotionum were obtained and identified as Nodulisporium sp. by its morphological characters and ITS molecular sequences. The carbon sources that this fungus relied on were diverse and it grew well by any of maltose,fructose,glucose and sucrose as carbon resource. There existed significant differences among nitrogen sources for this fungus’ growth,and the optimal nitrogen source was beef extract. The optimal temperature was 25℃,and the hypha was inhibited significantly when the temperature fell below 10℃or rose above 30℃. This fungus also tolerated very wide range of pH,and grew well under pH 5 to 9.

Key words: Sclerotinia rot of colza, biological control, culture in vitro, endophytic fungi, Orchidaceae

DING Rui, ZHANG Hao-qi, CHEN Xu-hui, LIN Hao, QU Bo. Identification and Biological Characteristics of One Antagonistic Fungal Strain Against Sclerotinia sclerotiorum[J]. Biotechnology Bulletin, 2018, 34(4): 174-178.

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