Screening of Microsatellite Markers Associated with Furfural Tolerance of Saccharomyces cerevisiae

doi:10.13560/j.cnki.biotech.bull.1985.2018-0107

Abstract

Abstract: Two phenotypically different haploid strains were screened as parents and were crossed to obtain two hundred segregants. The polymorphic microsatellite markers were used to amplify offspring yeasts（39 strains）,and t-test was used to analyze the significance of difference among the genotypes of different sites in two gene pools,which is aimed to have microsatellite markers associated with furfural tolerance. The results revealed that two microsatellite markers associated with furfural tolerance were identified. Among them the marker 2P3 had significant differences（P = 0.039 < 0.05）and marker 2P5 had extremely significant differences（P = 0.000 ﹤ 0.01）in the 2 gene pools. Furthermore,72.2% of high tolerant individuals contained the marker 2P5（333 bp）derived from P1,the high tolerant parent;100% of low tolerant individuals contained the marker 2P5（318 bp）derived from P2,the low tolerant parent. These results suggested that the significant difference of 2P5 genotype in population segregation may be linked with the gene controlling this trait and can be used to select the favorable allele in molecular design and breeding so as to improve the accuracy and predictability of breeding.

Key words: Saccharomyces cerevisiae, furfural tolerance, phenotypic analysis, SSR markers

LI Sha-sha, ZHANG Liang, SHI Gui-yang. Screening of Microsatellite Markers Associated with Furfural Tolerance of Saccharomyces cerevisiae[J]. Biotechnology Bulletin, 2018, 34(8): 123-129.

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