Research Progress in Quantitative and Unitive Detecting Technologies of Functional Nucleic Acid and Label-Free Fluorescence

doi:10.13560/j.cnki.biotech.bull.1985.2018-0809

Abstract

Abstract: Functional nucleic acid is a natural or an artificial nucleic acid sequence with specific spatial conformation and biological function. It has advantages such as easy method to modify,low cost,high stability and strong specificity. After combined with label-free fluorescence biosensors,the functional nucleic acid performs the function of transferring all different kinds of targets to stable signals of nucleic acid and amplifying signals by the amplification of nucleic acid. In addition,the complexity of process and cost from labeling and screening of fluorescence,quenching groups in label-fluorescence can be avoided by the technology of label-free fluorescence,while the fluorescence signal transfer can be ensured by the specific or unspecific combination of nucleic acid and fluorescence. Combining the advantages of 2 technologies,the sensitivity and real-time capability can be enhanced,and the functional nucleic acid-based label-free fluorescence biosensors have been widely applied in the detection of environmental pollutants,risk factors of food safety,disease diagnosis,etc. Firstly,we define the concepts of functional nucleic acid and quantitative fluorescence detecting technology,and elaborate in detail the features of key fluorescent materials,as well as their biological molecular recognitions,reaction principles,and luminous models with functional nucleic acid. Then,we describe,assesse,and compare the quantitative and unitive detecting technologies of functional nucleic acid and label-free fluorescence and their applications according to their features. Finally,we discuss the research significance and issues of the quantitative and unitive detecting technologies of functional nucleic acid and label-free fluorescence in detections,and prospect the future development and application.

Key words: functional nucleic acid, label-free fluorescence, quantitative detection, biosensor

XIAO Bing, LIU Bang, LUO Yun-bo, HUANG Kun-lun, ZHANG Yuan, LI Xia-ying, ZHANG Xiu-jie, XU Wen-tao, ZHOU Xiang. Research Progress in Quantitative and Unitive Detecting Technologies of Functional Nucleic Acid and Label-Free Fluorescence[J]. Biotechnology Bulletin, 2019, 35(3): 194-202.

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