SNP Identification of MyoD1 Gene and Its Correlation with Growth Traits in Pelodiscus sinensis

doi:10.13560/j.cnki.biotech.bull.1985.2018-0907

Abstract

Abstract: In order to study the correlation between DNA polymorphisms of MyoD1 gene and growth traits of P. sinensis,direct sequencing method was used and 6 SNP loci（T-49G,A-38G,C91T,A187T,C880T and T1522A）in P. sinensis MyoD1 gene were identified. Among them,one SNP（C880T）was located in exons and missense mutation happened. Randomly selecting 178 randomly 2-winter-age P. sinensis bred in the same batch and cultivated in the same rice field,the genotypes of each SNP locus of them were detected. The results showed that the average effective number of allele（Ne）,average observed heterozygosity（Ho）,and average expected heterozygosity（He）of these SNPs was 1.636 5,0.349 3 and 0.375 4,respectively. Chi-square test showed that 5 SNP loci（T-49G,A-38G,C91T,C880T,and T1522A）were in Hardy-Weinberg equilibrium state in P. sinensis population. A general linear model was established for correlation analysis between different genotypes at SNP loci of MyoD1 gene and various growth traits. At the locus of T-49G,individuals with the GG genotype had significantly greater carapace widths than ones with the TT genotypes. At the locus of A-38G,individuals with the AG genotype had significantly greater carapace widths than ones with the AA genotypes. At the locus of A187T,individuals with the TT genotype had significantly greater heights than ones with the genotype AA. At the locus of T1522A,individuals with the AA genotype had significantly greater body weights than ones with the TT and TA genotypes. Other three SNP loci were not significantly correlated with growth traits. The growth-related locus T-49G,A-38G,A187T and T1522A could be useful candidate molecular markers in P. sinensis molecular marker-assisted selection.

Key words: Pelodiscus sinensis, myogenic differentiation 1 gene, single nucleotide polymorphism, growth traits, correlation analysis

HUANG Long, WU Ben-li, HE Ji-xiang, CHEN Jing, SONG Guang-tong, WANG Xiang, ZHANG Ye, WU Song. SNP Identification of MyoD1 Gene and Its Correlation with Growth Traits in Pelodiscus sinensis[J]. Biotechnology Bulletin, 2019, 35(4): 76-81.

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