Correlation Analysis Between LHX3 Gene Polymorphism and Growth Traits in Three Sheep Breeds

doi:10.13560/j.cnki.biotech.bull.1985.2019-0384

Abstract

Abstract: This work aims to provide a theoretical basis for high quality sheep breeding by finding molecular markers related to the sheep growth traits. Agarose gel electrophoresis and first-generation sequencing technology were used to detect the three genotypes of LHX3 genes in Tan sheep（TS）,Small-Tailed Han sheep（STHS）and Lanzhou Fat-Tailed sheep（LFTS）,which include insertion（AA）,insertion/deletion（AB）and deletion（BB）of 29 bp in LHX3 gene. Genetic polymorphism of LHX3 in three different sheep breeds was analyzed and the correlation between LHX3 different genotypes and the growth traits of three different breeds were analyzed by general linear model. The polymorphic information content（PIC）of LHX3 in the different breeds was as 0.25<PIC≤0.5,indicating a high genetic variation of this point. The LHX3 gene affected one or more growth traits in the three different sheep breeds. In Tan Sheep,the body length（BL）,body weight（BW）,chest depth（ChD）and cannon circumference（CaC）were significantly higher（P<0.05）in LHX3 gene deletion genotype than insertion genotype and insertion/deletion genotype. In Small-Tailed Han sheep,the chest circumference（ChC）,ChD and CaC were significantly higher（P<0.01）in LHX3 gene deletion genotype than the other two genotypes. In Lanzhou Fat-Tailed sheep,the ChC,BL,BW,hip width（HW）and CaC were significantly higher（P<0.01）in LHX3 gene deletion genotype than the other two genotypes. The 29 bp-deletion genotype in LHX3 may be selected as a DNA marker site in sheep molecular breeding.

Key words: sheep, LHX3 gene, gene polymorphism, correlation analysis, growth traits

WANG Yan-xin, LIAO Yuan-yuan, A Yimuguli, QI Ao-qiong, LI Hai-jian, XU Hong-wei, YANG Ju-tian, CAI Yong. Correlation Analysis Between LHX3 Gene Polymorphism and Growth Traits in Three Sheep Breeds[J]. Biotechnology Bulletin, 2019, 35(10): 162-168.

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