Research Progress in Fusion Modification of PAS

doi:10.13560/j.cnki.biotech.bull.1985.2019-0519

Abstract

Abstract: PAS protein modification technology refers to that functional polypeptides with polypeptide sequences composed of proline,alanine and serine by gene fusion are linked and fusion expression is conducted by gene engineering,thus polypeptide modification is achieved. At present,for small molecule polypeptide drugs,its smaller volume leads to a shorter half-life in plasma,and the clinical application of PEG to modify small molecule polypeptide drugs makes it have a larger hydrodynamic volume and thus its pharmacological activity is increased. However,with more and more PEG modified drugs entering the clinical application market in recent years,the safety drawbacks of PEG chemical polymers have been constantly uncovered. Therefore,it is necessary to seek more safe and effective methods besides PEG modification,and PAS has shown great advantages in small molecular polypeptide modification. In this paper,the design ideas and advantages of PAS protein modification technology,as well as the latest progress in application are described in detail.

Key words: PASylation, fusion expression, half-life, advantages, application

HAN Fei, JIANG Ming-feng, RAN Rong, WANG Gang. Research Progress in Fusion Modification of PAS[J]. Biotechnology Bulletin, 2019, 35(12): 152-158.

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