Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (11): 42-56.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1158

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Sequencing and Analysis of the Whole Genome of Zizhi Cultivar ‘Wuzhi No.2’(Ganoderma sp. strain Zizhi S2)

CHEN Ti-qiang1(), XU Xiao-lan2, SHI Lin-chun3, ZHONG Li-Yi4   

  1. 1. Institute of Edible & Medicinal Fungi Fujian Academy of Agricultural Sciences,Fuzhou 350014
    2. Department of traditional Chinese medicine resources and bee products College of Animal Science College of Bee Science Fujian Agriculture and Forestry University,Fuzhou 350002
    3. Institute of Medicinal Plant Development Chinese Academy of Medical Sciences,Beijing 100193
    4. Fujian Wuping Mushroom Technical Advice Station,Longyan 364300
  • Received:2021-09-09 Online:2021-11-26 Published:2021-12-03
  • Contact: CHEN Ti-qiang E-mail:chen_tiqiang@189.cn

Abstract:

The cultivar ‘Wuzhi No. 2(original name:S2)’ has been applied and extended in south China(Fujian,Guangdong and Guangxi province,etc.),known as Ganoderma sp. strain Zizhi S2 that belongs to Ganoderma sinense complex. Whole genome of the Zizhi S2 was completed after genomic sequencing and assembly using the PacBio SMRT technology and based on the third-generation and second-generation data(3 + 2 strategy),the total length of the sequence was 56.849 Mb,totally 72 scaffolds including nuclear genome and mitochondrial genome. There were 16 681 genes in the nuclear genome,and 13 161 genes were annotated and accounting for 78.89% of the total. Four groups of tandem repeat rRNA clusters,405 non-coding RNAs and 3 305 promoters were predicted and analyzed. The gene sequences of rpb2,beta-tubulin and 18S,28S rRNA(SSU,LSU),laccase isoenzyme genes,squalene synthase and lanosterol synthesis genes,ergo-sterol synthesis and metabolism related genes and their distribution positions were revealed. This finding not only provides reliable information for the molecular identification and strain identification of cultivated varieties(lines)of Zizhi,but also for further mining and utilization of functional genes. Furthermore,this also accumulates scientific data for the subsequent comparative genomic analysis of cultivated Ganoderma spp. complex species in China.

Key words: Zizhi(Ganoderma sinense complex), cultivar, whole genome sequencing, genomic components, gene functional annotation