Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (6): 198-210.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1151

Previous Articles     Next Articles

Transcriptome Sequencing in the Leaves of Pontederia cordata with Cadmium Exposure and Gene Mining in Phenypropanoid Pathways

XIN Jian-pan(), LI Yan, ZHAO Chu, TIAN Ru-nan()   

  1. College of Architecture Landscape,Nanjing Forestry University,Nanjing 210037
  • Received:2021-09-07 Online:2022-06-26 Published:2022-07-11
  • Contact: TIAN Ru-nan E-mail:jianpanxin@njfu.edu.cn;tianrunan@njfu.edu.cn

Abstract:

To provide theoretical base for the mechanism of phenylpropane metabolic pathway involved in Pontederia cordata against heavy metal exposure,and to analyze the transcriptome sequences in the leaves of P. cordata exposed to cadmium(Cd),the obtained Unigenes were compared and annotated with seven databases including NR,NT,PFAM,KOG,Swiss Prot,and KEGG as well as GO,and the tendency of differentially expressed genes(DEGs)were analyzed,and DEGs involved in phenypropanoid metabolic pathway were mined. Results showed as follows. 1)A total of 221 392 unigenes were obtained,of which 170 175 unigenes were successfully annotated in the public database,and 6 506 unigenes were annotated for 31 secondary metabolic pathways. 2)A total of 168 355 were obtained after comparing the Unigenes from the leaves of P. cordata with dadabase Swiss Prot and NR. And 84 673 CDS sequences were obtained by Esacan software. 3)Trend analysis demonstrated that 20 025 DEGs were clustered into three significant profiles including two down-regulated profiles(cluster 0 covering 2 631 DEGs and cluster 1 covering 3 153 DEGs)and one up-regulated profiles(cluster 5 covering 3 733 DEGs). 4)With Cd2+ the exposure,there were 26 DEGs identified in P. cordata leaves in 6 h and 48 h by further mining the transcriptome data,which encoded 3 crucial enzymes in the common pathway of phenylpropane metabolism,5 crucial enzymes related to lignin biosynthesis,and 7 crucial enzymes responsible for flavonoid biosynthesis. P. cordata is involved in the accumulation and detoxification of Cd in the leaves by regulating the biosynthesis of guaiacyl lignin and up-regulating the gene expression of cinnamate-4-hydroxylase(C4H),anthocyanin synthase(ANS),anthocyanin-3-O -glucosyltransferase(3GT),caffeonyl coenzyme A-O methyltransferase(CCoAOMT),and flavonol 3-methyltransferase(F3OMT). The genes involved in phenylpropane metabolic pathway in the leaves of P. cordata against Cd stress is preliminarily revealed by transcriptome sequencing,which provides a theoretical basis for further research on the function of phenylpropane metabolic pathway in the resistance of the plant to heavy metal stress and related mechanisms.

Key words: cadmium, Pontederia cordata, transcriptome in leaves, phenypropanoid pathway, gene mining